Hierarchical Sorter for High Purity Stem Cell Selection

用于高纯度干细胞选择的分层分选机

• 批准号: 6789056
• 负责人: MANISH DESHPANDE
• 金额: $ 10万
• 依托单位: CYTONOME, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6789056
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; cell sorting; cell transplantation; clinical biomedical equipment; hematopoietic stem cells; immunotherapy; neoplasm /cancer therapy; stem cell transplantation; stem cells; tissue /cell preparation

DESCRIPTION (provided by applicant): There is considerable evidence for the application of transplantation of Hematopoietic and Mesenchymal Stem Cells in the treatment of a wide range of cancers including lymphoma, neuroblastoma, and multiple myeloma. In addition, HSC/MSC therapies can be applied to reconstitute the immune system, regenerate damaged tissues, and deliver genes to repair damaged cells for many auto-immune diseases such as diabetes, rheumatoid arthritis, and system lupus erythematosis. One of the major roadblocks in the successful application of transplant treatments is the difficulty in obtaining a pure sample of stem cells in the harvested sample. This results in reinfusion of a larger number of residual tumor cells into the patient than is desirable, resulting, in some cases, in relapse of the disease. The ability to acquire a pure stem cell sample is therefore highly desirable. This research is focussed specifically on the development of a high-purity cell sorted for stem cell harvesting. The cell sorter uses a hierarchical multistage strategy with each sorting stage operating on the results of the previous sort thus reintorraging a sorted sample to verify its accuracy. The overall objective of this research is a sorter that results in a sample with greater than 99.9% purity through the use of multiple sorts.

描述（由申请人提供）：有大量证据表明造血干细胞和间充质干细胞移植可用于治疗多种癌症，包括淋巴瘤、神经母细胞瘤和多发性骨髓瘤。此外，HSC/MSC疗法还可用于重建免疫系统、再生受损组织、传递基因修复受损细胞，治疗糖尿病、类风湿性关节炎、系统性红斑狼疮等多种自身免疫性疾病。成功应用移植治疗的主要障碍之一是难以在收获的样品中获得干细胞的纯样品。这导致将比期望数量更多的残留肿瘤细胞回输到患者体内，在某些情况下导致疾病复发。因此，非常需要获得纯干细胞样本的能力。 这项研究专门致力于开发用于干细胞收获的高纯度细胞分选。细胞分选机采用分层多级策略，每个分选阶段都对前一分选的结果进行操作，从而重新存储分选的样本以验证其准确性。这项研究的总体目标是建立一种分选机，通过使用多种分选来获得纯度高于 99.9% 的样品。