A Shared Neuroscience Platform for National Dissemination and Training in Brain Organogenesis, Behavioral and Brain Disease Models, Viral Vectors, and Imaging Technologies

一个共享神经科学平台，用于脑器官发生、行为和脑疾病模型、病毒载体和成像技术的全国传播和培训

• 批准号: 10439384
• 负责人: William T Newsome
• 金额: $ 135.87万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-16 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10439384
• 关键词: 3-Dimensional; Acceleration; Aging; Area; Array tomography; Autopsy; Basic Science; Behavior; Behavioral; Behavioral Model; Biological Assay; Biotechnology; Brain; Brain Diseases; Brain region; Cell Communication; Cell Line; Cell model; Cells; Central Nervous System Diseases; Charge; Clinical; Clinical Research; Collaborations; Communities; Complementary DNA; Complex; Country; Custom; Development; Disease Progression; Disease model; Educational workshop; Emerging Technologies; Evaluation; Funding; Generations; Genes; Genetic; Genetic Models; Genomics; Goals; Grant; Human; Image; Image Analysis; Imaging Techniques; Imaging technology; Individual; Industry Standard; Institution; Instruction; International; Investigation; Laboratories; Learning; Longitudinal Studies; Maintenance; Memory; Methods; Microscopy; Mission; Missouri; Modality; Modeling; Molecular; Mus; Nervous system structure; Neurodegenerative Disorders; Neurosciences; Neurosciences Research; Opiate Addiction; Organogenesis; Organoids; Participant; Pathology; Peer Review; Pennsylvania; Persons; Phenotype; Pilot Projects; Plasmids; Preparation; Production; Proteomics; Publications; Reagent; Recombinant Proteins; Reproducibility; Research; Research Design; Research Personnel; Research Project Grants; Resource Sharing; Resources; Rodent; Running; Scholarship; Scientist; Services; Signal Transduction; Site; Slice; Standardization; Stem Cell Research; Stroke; Supervision; System; Tauopathies; Techniques; Technology; Testing; Texas; Three-Dimensional Imaging; Time; Tissues; Touch sensation; Training; Transgenic Organisms; Translational Research; Transplantation; United States National Institutes of Health; Universities; Validation; Viral; Viral Vector; Virus; Vision; Work; animal tissue; base; behavior test; behavioral phenotyping; behavioral study; brain tissue; clinically relevant; cloud based; computerized data processing; drug candidate; efficacy study; experimental study; functional outcomes; genetic manipulation; hands on instruction; human pluripotent stem cell; imaging system; improved; in vitro Model; in vivo; innovation; innovative technologies; knock-down; member; microscopic imaging; molecular dynamics; neuroimaging; neuronal circuitry; new technology; novel; novel strategies; novel therapeutics; optogenetics; outreach; programs; sharing platform; social; three dimensional cell culture; three dimensional structure; tool; underrepresented minority student; vector; viral gene delivery; virus core; web site

Advances in neuroscience depend on robust in vivo and in vitro models with innovative technologies to carry out functional and mechanistic studies accompanied by advanced imaging techniques. The Human Brain Organogenesis Program (HBOP), Behavioral and Functional Neuroscience Laboratory (BFNL), Gene Vector and Virus Core (GVVC), and Neuroscience Microscopy Services (NMS) make up a platform, the Stanford Neuroscience Research Center (SNRC), for centralization and dissemination of innovative neuroscience models, reagents and methods. The vision of SNRC is to provide an integrated platform in which users can expand their research to areas outside their expertise or engage multiple modalities in their research, such as applying viral vector approaches and neuroimaging approaches to behavioral models of disease or human organoid cultures. SNRC outreach will approach Neuroscience departments nationally with a call for applications for 6 fully-funded merit-based pilot studies designed to engage diverse SNRC resources. We will also offer comprehensive workshops on techniques and teach participants how to apply and integrate novel approaches into their current research programs. SNRC is strategically equipped with resources to provide critical support to a range of national research projects and has supported over 500 labs nationwide with over 200 peer-reviewed publications in the last decade. SNRC has a growing national user base from institutions including Yale, Harvard, University of Missouri, Cornell, Princeton, Columbia, University of Pennsylvania, University of Texas, MIT, and many more. Investigators anywhere in the world can request a viral vector, phenotype rodent lines, or have an in vivo stroke study or an efficacy study in a model of neurodegenerative disease run remotely. Anyone can attend a workshop for training in behavioral models, 3D imaging of whole brains, or methods for 3D human cellular models (organoids and assembloids). SNRC supports many small and large biotech companies in proof of concept efficacy studies of clinical drug candidates. This contribution has supported the advancement of these projects to human clinical studies. Through SNRC, academic users will have access to these same industry- standard efficacy studies. All SNRC activities will be supervised by a steering committee consisting of external, internal, and NIH members. Under this U24 grant, we will disseminate essential and cutting-edge resources. We will expand our external user network to share emerging technologies with the Pilot Study program and Annual Workshops. BFNL will expand its automated testing and data processing capabilities, including the Digilab cloud- based automated behavioral phenotyping system. GVVC will set up large-scale viral production as well as higher-level purification technology. NMS will include training classes in STARmap genomic imaging and array tomography proteomic imaging. Under this program, SNRC will engage with the national neuroscience community via newly improved resource sharing websites, hands-on instruction, and annual in-person or online workshops to facilitate networks for collaboration and acceleration of discoveries in brain function and pathology.

神经科学的进步取决于具有创新技术的体内和体外模型的强大模型 功能和机械研究伴随着高级成像技术。人脑 器官发生程序（HBOP），行为和功能性神经科学实验室（BFNL），基因载体 和病毒核心（GVVC）和神经科学显微镜服务（NMS）组成一个平台，斯坦福 神经科学研究中心（SNRC），用于创新神经科学的集中和传播 模型，试剂和方法。 The vision of SNRC is to provide an integrated platform in which users can 将他们的研究扩展到其专业知识以外的领域或参与其研究的多种方式，例如 将病毒载体方法和神经影像学方法应用于疾病或人类的行为模型 器官培养。 SNRC outreach will approach Neuroscience departments nationally with a call for applications 对于6个完全资助的基于绩效的试点研究，旨在参与多样化的SNRC资源。我们还将提供 有关技术的全面讲习班，并教参与者如何应用和整合新方法 进入他们当前的研究计划。 SNRC在战略上配备了资源，为 一系列国家研究项目，并为全国500多个实验室提供了200多个同行评审的实验室 过去十年的出版物。 SNRC的国家用户群来自包括耶鲁大学，哈佛，包括的机构 密苏里大学，康奈尔大学，普林斯顿，哥伦比亚，宾夕法尼亚大学，德克萨斯大学，麻省理工学院， 更多。世界上任何地方的调查人员都可以要求病毒载体，表型啮齿动物线或具有 体内中风研究或神经退行性疾病模型中的疗效研究远程运行。任何人都可以 参加用于行为模型培训的研讨会，全脑的3D成像或3D人类的方法 细胞模型（器官和组装）。 SNRC supports many small and large biotech companies in proof 临床药物候选物的概念疗效研究。这项贡献支持了这些贡献 项目进行人类临床研究。通过SNRC，学术用户将可以访问这些同一行业 - 标准功效研究。所有SNRC活动将由指导委员会监督，该指导委员会由外部， 内部和NIH成员。根据这笔U24赠款，我们将传播基本和尖端的资源。我们 将扩展我们的外部用户网络，以与试点研究计划和年度共享新兴技术 讲习班。 BFNL将扩展其自动测试和数据处理功能，包括Digilab Cloud- 基于自动行为表型系统。 GVVC将建立大规模的病毒生产以及 高级纯化技术。 NMS将包括Starmap基因组成像和数组中的培训课程 断层扫描蛋白质组学成像。根据该计划，SNRC将与国家神经科学互动 通过新改进的资源共享网站，动手指导以及年度面对面或在线的社区 讲习班，以促进网络，以协作和加速大脑功能和病理学的加速。