NE-CAT: A Resource for Advanced Macromolecular Crystallography

NE-CAT：高级高分子晶体学资源

• 批准号: 10505648
• 负责人: Nozomi Ando
• 金额: $ 379.29万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-04-01 至 2028-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10505648
• 关键词: 20 year old; Advisory Committees; Aging; Area; Automatic Data Processing; Biological; Biological Process; Biomedical Research; Characteristics; Communities; Computer software; Crystallography; Data Analyses; Data Collection; Dedications; Development; Education and Outreach; Educational workshop; Ensure; Funding; Goals; Grouping; Healthcare; Individual; Infrastructure; Institution; Logistics; Mainstreaming; Maintenance; Manuscripts; Modification; Optics; Paper; Persons; Pharmacologic Substance; Photons; Productivity; Professional Organizations; Publications; Publishing; Recruitment Activity; Research; Research Personnel; Research Project Grants; Resources; Robotics; Roentgen Rays; Running; Sampling; Schedule; Site; Source; Structure; Synchrotrons; System; Techniques; Technology; Time; Training; Training Activity; Training Programs; United States National Institutes of Health; Universities; Work; beamline; biological systems; computerized data processing; data structure; design; detector; experience; improved; macromolecule; meetings; member; operation; outreach; outreach program; programs; repaired; three dimensional structure; three-dimensional visualization; web based interface; web site

The main goal of NE-CAT is to provide user access to state-of-the-art synchrotron beamlines for macromolecular crystallography. NE-CAT began planning and design for two beamlines in 2001. Beamline 24-ID-C became available to Advanced Photon Source users in 2006 and beamline 24-ID-E became available in 2007. During the ensuing years, beamline improvements have resulted in stable, small X-ray beams ideally suited for challenging problems in macromolecular crystallography. Each beamline is equipped with a high capacity sample automounter and a microdiffractometer. Beamline 24-ID-C features an EIGER2 X 16M pixel array detector, and 24-ID-E features an EIGER X 16M. NE-CAT provides automated software for data collection and analysis (RAPD) and a web-based interface for remote data collection. We will continue to provide routine maintenance to ensure efficient and reliable data collection, and we propose an upgrade program to maintain state-of-the-art capabilities over the five years covered by this application. This period includes 12 months without X-rays required for the APS upgrade project. We will use this superb opportunity to rebuild a nearly 20-year-old system to be leaders in the field for another 20 years. NE-CAT has an experienced and dedicated scientific staff who enable access to the beamlines. The staff is available or on call 24/7 to assist users with data collection and processing needs. This highly regarded staff is a major strength of NE-CAT. NE-CAT has an outstanding record of reliability with technical problems rarely interfering with user access. NE-CAT is directed by Dr. Steven Ealick, who has more that 30 years of experience overseeing the operation of synchrotron facilities for macromolecular crystallography. The technical group, led by Deputy Director Dr. Malcolm Capel, and the operations group, led by Associate Director Dr. Frank Murphy work closely together to ensure that the needs of the user community are met. Together, these three individuals make up the NE-CAT management team. NE-CAT beam time is in high demand, and each run all available beam time is allocated to users. NE-CAT typically hosts more than 1500 users per year from about 160 unique user groups, either on site or remotely. NE- CAT users published about 200 papers during the last year and are projected to publish more than 190 in 2021. NE-CAT’s record of productivity places it among the top of worldwide facilities for macromolecular crystallography. NE-CAT operates an extensive user training program adaptable to first time users, advanced users with technically challenging problems, and everyone in between. A website is available to provide all information needed by new or current users. NE-CAT participates in many training and outreach activities including organization of an annual workshop and participation in professional society meetings. The staff works closely with user groups and are often invited to coauthor manuscripts resulting from user support and training.

NE-CAT 的主要目标是为用户提供最先进的同步加速器光束线 NE-CAT 于 2001 年开始规划和设计两条光束线。 24-ID-C 于 2006 年向高级光子源用户提供，光束线 24-ID-E 成为 于 2007 年推出。在接下来的几年中，光束线的改进带来了稳定的小型 X 射线 光束非常适合解决高分子晶体学中的挑战性问题。 Beamline 24-ID-C 配备高容量样品自动安装器和微衍射仪。 EIGER2 X 16M 像素阵列探测器，24-ID-E 具有 EIGER X 16M NE-CAT 功能。 用于数据收集和分析 (RAPD) 的自动化软件以及用于远程数据的基于 Web 的界面 我们将继续提供日常维护，以确保数据收集高效可靠， 我们提出了一项升级计划，以在五年内保持最先进的能力 此申请期间包括 APS 升级项目所需的 12 个月。 将利用这个绝佳的机会重建一个拥有近20年历史的系统，成为另一个领域的领导者 20 年来，NE-CAT 拥有一支经验丰富、敬业的科学人员，能够接触到光束线。 工作人员 24/7 随时待命，协助用户满足数据收集和处理需求。 受人尊敬的员工是 NE-CAT 的主要优势 NE-CAT 拥有出色的技术可靠性记录。 NE-CAT 是由 Steven Ealick 博士指导的，他拥有更多相关知识。 拥有 30 年监督高分子同步加速器设施运行的经验 由副主任 Malcolm Capel 博士领导的技术小组和运营小组， 由副总监 Frank Murphy 博士领导，密切合作，确保用户的需求 这三人共同组成了 NE-CAT 管理团队。 波束时间的需求量很大，每次运行时，所有可用的波束时间通常都会分配给用户。 每年接待来自约 160 个独特用户组的 1500 多名用户，无论是现场还是远程。 CAT 用户去年发表了约 200 篇论文，预计将在 2019 年发表超过 190 篇论文。 2021 年。NE-CAT 的生产力记录使其跻身全球高分子设施之列 NE-CAT 提供适合初次用户的广泛培训计划， 具有技术挑战性问题的高级用户，以及介于两者之间的每个人都可以使用该网站。 提供新用户或现有用户所需的所有信息 NE-CAT 参与许多培训和外展活动。 活动包括组织年度研讨会和参加专业协会会议。 工作人员与用户团体密切合作，经常受邀共同撰写用户的手稿 支持和培训。

项目成果

Structural basis of colibactin activation by the ClbP peptidase.

• DOI: 10.1038/s41589-022-01142-z
• 发表时间: 2023-02
• 期刊: NATURE CHEMICAL BIOLOGY
• 影响因子: 14.8
• 作者: Velilla, Jose A.;Volpe, Matthew R.;Kenney, Grace E.;Walsh, Richard M., Jr.;Balskus, Emily P.;Gaudet, Rachelle
• 通讯作者: Gaudet, Rachelle

Discovery of Nanomolar DCAF1 Small Molecule Ligands.

• DOI: 10.1021/acs.jmedchem.2c02132
• 发表时间: 2023-04-13
• 期刊: JOURNAL OF MEDICINAL CHEMISTRY
• 影响因子: 7.3
• 作者: Li, Alice Shi Ming;Kimani, Serah;Wilson, Brian;Noureldin, Mahmoud;Gonzalez-Alvarez, Hector;Mamai, Ahmed;Hoffer, Laurent;Guilinger, John P.;Zhang, Ying;von Rechenberg, Moritz;Disch, Jeremy S.;Mulhern, Christopher J.;Slakman, Belinda L.;Cuozzo, John W.;Dong, Aiping;Poda, Gennady;Mohammed, Mohammed;Saraon, Punit;Mittal, Manish;Modh, Prati k;Rathod, Vaibhavi;Patel, Bhashant;Ackloo, Suzanne;Santhakumar, Vijayaratnam;Szewczyk, Magdalena M.;Barsyte-Lovejoy, Dalia;Arrowsmith, Cheryl H.;Marcellus, Richard;Guie, Marie-Aude;Keefe, Anthony D.;Brown, Peter J.;Halabelian, Levon;Al-awar, Rima;Vedadi, Masoud
• 通讯作者: Vedadi, Masoud

Identification of the bacteriophage nucleus protein interaction network.

• DOI: 10.1038/s41594-023-01094-5
• 发表时间: 2023-11
• 期刊: NATURE STRUCTURAL & MOLECULAR BIOLOGY
• 影响因子: 16.8
• 作者: Enustun, Eray;Deep, Amar;Gu, Yajie;Nguyen, Katrina T.;Chaikeeratisak, Vorrapon;Armbruster, Emily;Ghassemian, Majid;Villa, Elizabeth;Pogliano, Joe;Corbett, Kevin D.
• 通讯作者: Corbett, Kevin D.

Atypical chemoreceptor arrays accommodate high membrane curvature.

• DOI: 10.1038/s41467-020-19628-6
• 发表时间: 2020-11-13
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Muok AR;Ortega DR;Kurniyati K;Yang W;Maschmann ZA;Sidi Mabrouk A;Li C;Crane BR;Briegel A
• 通讯作者: Briegel A

Shark nanobodies with potent SARS-CoV-2 neutralizing activity and broad sarbecovirus reactivity.

• DOI: 10.1038/s41467-023-36106-x
• 发表时间: 2023-02-03
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Chen, Wei-Hung;Hajduczki, Agnes;Martinez, Elizabeth J.;Bai, Hongjun;Matz, Hanover;Hill, Thomas M.;Lewitus, Eric;Chang, William C.;Dawit, Layla;Peterson, Caroline E.;Rees, Phyllis A.;Ajayi, Adelola B.;Golub, Emily S.;Swafford, Isabella;Dussupt, Vincent;David, Sapna;Mayer, Sandra V.;Soman, Sandrine;Kuklis, Caitlin;Corbitt, Courtney;King, Jocelyn;Choe, Misook;Sankhala, Rajeshwer S.;Thomas, Paul V.;Zemil, Michelle;Wieczorek, Lindsay;Hart, Tricia;Duso, Debora;Kummer, Larry;Yan, Lianying;Sterling, Spencer L.;Laing, Eric D.;Broder, Christopher C.;Williams, Jazmean K.;Davidson, Edgar;Doranz, Benjamin J.;Krebs, Shelly J.;Polonis, Victoria R.;Paquin-Proulx, Dominic;Rolland, Morgane;Reiley, William W.;Gromowski, Gregory D.;Modjarrad, Kayvon;Dooley, Helen;Joyce, M. Gordon
• 通讯作者: Joyce, M. Gordon