Analysis of embryonic brain wiring in C. elegans

线虫胚胎大脑线路分析

• 批准号: 10355924
• 负责人: JOSHUA M KAPLAN
• 金额: $ 58.8万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10355924
• 关键词: Address; Behavior; Behavioral; Biological Assay; Biology; Brain; Caenorhabditis elegans; Cells; Chemical Synapse; Clustered Regularly Interspaced Short Palindromic Repeats; Cognition; Coupled; Defect; Development; Drug Exposure; Embryo; Embryonic Development; Event; Exhibits; Fertilization; Fetal Tissues; Frequencies; Ganglia; Gene Expression; Genes; Genetic; Goals; Head; Health; Hour; Human; Image; Infection; Larva; Link; Mediating; Modeling; Molting; Motion; Mutation; Neurodevelopmental Disorder; Neurons; Pattern; Physiological; Physiology; Play; Property; Research Personnel; Risk; Role; Skin; Sleep; Sleep Disorders; Sleep disturbances; Synapses; Testing; Time; associated symptom; autism spectrum disorder; behavioral outcome; critical period; experimental study; fetal; hatching; insight; larval control; neural circuit; prevent; screening; skin organogenesis; synaptogenesis; tool

Analysis of embryonic brain wiring in C. elegans The goal of this project is to investigate how neural circuits are assembled in C. elegans embryos. We develop assays for an embryonic behavior (a sleep-like quiescent state) and for the neurons controlling this behavior. This embryonic sleep state is analogous to the quiescence that occurs during larval molts. We will determine if quiescence is triggered by cuticle synthesis. We will image synapse formation in embryos and will determine if sleep circuit development is controlled by heterochronic genes (which control larval skin development). We will also ask if circuit development is altered by mutations that prevent sleep or by those linked to Autism spectrum disorders (ASD). These Aims address basic questions about the genetic and physiological mechanisms controlling early brain development.

线虫胚胎大脑线路分析 该项目的目标是研究线虫中的神经回路是如何组装的 胚胎。我们开发了胚胎行为（类似睡眠的静止状态）的检测方法 以及控制这种行为的神经元。这种胚胎睡眠状态类似于 幼虫蜕皮期间发生的静止。我们将确定是否静止 由角质层合成引发。我们将对胚胎中的突触形成进行成像，并将 确定睡眠回路的发育是否受异时基因控制（ 控制幼虫皮肤发育）。我们还将询问电路开发是否被改变 妨碍睡眠的突变或与自闭症谱系障碍 (ASD) 相关的突变。 这些目标解决有关遗传和生理机制的基本问题 控制早期大脑发育。