Telemetric Regenerative Bandage for Accelerating Wound Healing

用于加速伤口愈合的遥测再生绷带

基本信息

批准号：
10346507
负责人：
Guillermo Antonio Ameer
金额：
$ 64.9万
依托单位：
NORTHWESTERN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-24 至 2026-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10346507
关键词：
Acrylamides Address Adhesions Amputation Animal Model Animals Antioxidants Attenuated Bacterial Infections Bandage Biocompatible Materials Blood Circulation Body Temperature Caring Cells Chronic Citrates Clinic Clinical Complications of Diabetes Mellitus Copper Dermal Development Devices Diabetes Mellitus Diabetic Foot Ulcer Diabetic mouse Digit structure Disease Endothelial Cells Engineering Exposure to Family suidae Feedback Fibroblasts Free Radicals Gel Goals Health Healthcare High Prevalence Hospitals Human Immobilization Impaired healing Impaired wound healing Impairment Implant In Vitro Incidence Infection Inflammation Laminin Lead Left Limb structure Location Lower Extremity Measures Medical Device Metabolic syndrome Metals Miniature Swine Monitor Operative Surgical Procedures Oxidative Stress Patient-Focused Outcomes Patients Peptides Personal Satisfaction Physicians Polyethylene Glycols Population Positioning Attribute Prevalence Process Property Research Research Personnel Safety Sepsis Shapes Skin Skin wound healing Societies Specific qualifier value Sterile coverings Stretching System Telemetry Temperature Testing Thick Time Tissues Vascularization Wireless Technology Work Wound Infection angiogenesis base biomaterial compatibility blood perfusion clinically relevant db/db mouse diabetes amputation diabetic diabetic ulcer effective therapy flexibility healing health care delivery health care disparity human disease improved innovation keratinocyte limb amputation macromolecule microsensor migration mouse model nanoparticle nanoparticle delivery neovascularization non-healing wounds novel outcome prediction porcine model prevent regenerative research clinical testing scaffold sensor skin wound therapy outcome tissue regeneration tool wound wound bed wound care wound closure wound dressing wound healing

项目摘要

Summary Diabetic foot ulcers (DFUs) are a major complication of diabetes. These sores, if left untreated, can become infected and become a serious threat to the patient’s well being. Although the field of wound care management is well established, the effective treatment of chronic DFUs remains a challenge. The primary goal in the treatment of DFUs is for the wound to close as soon as possible and to do so in a durable way. However, prolonged inflammation, oxidative tissue damage, and impaired blood circulation in diabetic wounds delay the wound healing process, resulting in open, non-healing wounds that often lead to limb amputations. This proposal will address these problems by developing a versatile wound dressing that restores normal wound healing rates by reducing free radicals in the wound, providing a native-like scaffold for the cells to divide and migrate, and enhancing vascularization in the wound. Another problem is the inability to monitor the wound in real time after the patient leaves the hospital, leading to digit or limb amputations. We will address this problem by developing a wireless system that can monitor the temperature and pH of the wound in real time, parameters that have been shown to be indicators of infection. Therefore, the overall goal of this proposal is to develop a shape- conforming antioxidant dressing that upon exposure to body temperature transforms into a gel that promotes new tissue formation in diabetic wounds and a feedback system that involves tissue conforming sensors to monitor bacterial infection and/or lack of healing. Toward this goal, we have developed a novel macromolecule - poly (polyethylene glycol citrate-co-N isopropyl acrylamide) - that incorporates a laminin-derived peptide. This material, referred to as PPCN-A5G81, supports tissue regeneration and can conform to the unique shape and depth of a wound. As for wireless monitoring of the wound, we pioneered the development of flexible, stretchable electronic sensors that can be integrated with human skin or implanted into the body for continuous, non-invasive health monitoring and treatment of disease. We hypothesize that: 1) incorporating immobilized Cu2+ into PPCN-A5G81 will confer vasculoinductive properties that significantly increase PPCN-A5G81’s ability to restore normal healing rates of full thickness dermal wounds in diabetic mouse and swine models; and 2) conforming temperature and pH sensors are safe and can remotely provide real time information regarding blood perfusion and infection in dermal wounds in diabetic animals. The specific aims of this proposal are to: 1) fabricate a PPCN-based regenerative dressing with vasculoinductive, dermoconductive, and dermoinductive properties and investigate its safety and efficacy for healing full thickness wounds in diabetic mice and diabetic pigs with metabolic syndrome; and 2) fabricate and characterize telemetric wound feedback tissue-conforming sensors capable of measuring temperature and pH in infected and non-infected diabetic dermal wounds. Results from this research will contribute to the development of innovative clinical products that reduce amputation rates and improve patient outcome.

概括糖尿病足溃疡（DFU）是糖尿病的主要并发症。这些疮，如果不加以治疗，可能会变成感染并成为对患者健康的严重威胁。尽管伤口护理管理领域已经建立得很好，对慢性DFU的有效治疗仍然是一个挑战。主要目标 DFU的治疗方法是使伤口尽快关闭，并以持久的方式进行。然而，长时间感染，氧化组织损伤和糖尿病伤口血液循环受损延迟伤口愈合过程，导致开放的非治疗增长，通常会导致肢体截肢。这个建议通过开发一种多功能伤口敷料来恢复正常伤口愈合率，将解决这些问题通过减少伤口中的自由基，为细胞提供分裂和迁移的天然样支架，并增强伤口中的血管化。另一个问题是无法实时监测伤口患者离开医院，导致数字或肢体截肢。我们将通过开发解决这个问题可以实时监视伤口温度和pH的无线系统，参数已被证明是感染的指标。因此，该提案的总体目标是开发形状 - 符合抗氧化剂的敷料，暴露于体温后会变成凝胶促进糖尿病伤口和涉及组织的反馈系统中的新组织形成符合传感器以监测细菌感染和/或缺乏愈合。达到这个目标，我们有开发了一种新型的大分子 - 聚乙二醇柠檬酸-CO -N丙烯酰胺） - 结合层粘连蛋白衍生的肽。该材料（称为PPCN-A5G81）支持组织再生并可以符合伤口的独特形状和深度。至于伤口的无线监测，我们开发可以与人皮或可以集成的柔性，可拉伸的电子传感器的发展植入体内，以进行连续的，非侵入性的健康监测和疾病治疗。我们假设那：1）将固定的Cu2+掺入PPCN-A5G81中将赋予显着的血管诱导特性提高PPCN-A5G81恢复糖尿病小鼠全厚皮肤伤口正常愈合率的能力和猪模型； 2）符合温度和pH传感器是安全的，可以远程提供实时有关糖尿病动物皮肤伤口血液灌注和感染的信息。具体目的该建议是：1）用血管诱导的，皮肤传导性，制造基于PPCN的再生调味料，和皮肤诱导特性，并研究其在糖尿病中愈合全厚性伤口的安全性和效率小鼠和具有代谢综合征的糖尿病猪； 2）制造和表征远程远程伤口的反馈组织构成的传感器能够在感染和未感染的糖尿病中测量温度和pH 真皮伤口。这项研究的结果将有助于开发创新的临床产品降低截肢率并改善患者预后。