Flow Cytometry Laboratory Shared Resource

流式细胞术实验室共享资源

• 批准号: 9756883
• 负责人: Manish S Patankar
• 金额: $ 0.59万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9756883
• 关键词: Advisory Committees; Biological Assay; Biometry; Cancer Center; Cancer Center Support Grant; Cell Separation; Cells; Complex; Comprehensive Cancer Center; Computer Hardware; Computer software; Consult; Consultations; Custom; Cytometry; Data; Data Analyses; Data Set; Education; Educational workshop; Ensure; Etiology; Faculty; Feedback; Flow Cytometry; Fluorescence; Funding; Future; Generations; Image Cytometry; Informatics; Knowledge; Laboratories; Laboratory Personnel; Malignant Neoplasms; Mission; Monitor; NCI Center for Cancer Research; Population; Prevention; Procedures; Reagent; Reproducibility; Research; Research Support; Resource Sharing; Schedule; Services; Sorting - Cell Movement; Strategic Planning; Technology; Training; Universities; Vendor; Wisconsin; base; cancer therapy; cost; cost effective; data acquisition; design; experimental study; fluorophore; informatics shared resource; instrument; instrumentation; knowledge base; member; operation; programs; success

PROJECT SUMMARY / ABSTRACT The Flow Cytometry Laboratory (FLOW) was established by the UWCCC in 1988 to provide state-of-the-art instrumentation and expertise that enables our members to acquire and analyze complex data sets on single cells and cell populations and to isolate defined cell populations for further study. In the current CCSG funding cycle, FLOW has supported the research of 137 unique UWCCC members representing all 6 of our scientific programs. We have acquired new instrumentation, increased our capacity, established new services, and expanded our educational programs directed to UWCCC members, their trainees, and staff. We have engaged UWCCC leaders and our Scientific Advisory Committee to develop a strategic plan that will enable us to continue to excel in our mission and meet the rapidly evolving research needs of UWCCC members. Over the course of the next funding cycle we will achieve the following aims. Specific Aim 1 is to provide UWCCC members access to state-of-the-art instrumentation for single cell analytics and sorting. FLOW houses, operates, and maintains the broad assortment of instruments required for multi-parameter analytical cytometry, imaging cytometry, and cell sorting. FLOW has established Standard Operating Procedures (SOPs) that ensure all instruments are properly calibrated and operated in the manner required to yield high quality datasets that achieve accepted standards for experimental rigor and reproducibility. Specific Aim 2 is to provide expertise required for acquisition and analysis of multi-parameter flow cytometry data. Cell analytics and sorting technologies are advancing rapidly, and FLOW leaders monitor these advances and strive to ensure UWCCC members are able to fully exploit the newest technologies in their research. FLOW staff are experts in developing reagent panels that enable members to utilize our instruments to collect multi-parameter datasets with minimal spectral overlap. In addition, FLOW is developing a collaborative initiative with our Biostatistics and Cancer Informatics Shared Resources to ensure that the multi-parameter datasets generated on our instruments have appropriate power for robust statistical and informatics analyses. Specific Aim 3 is to provide expert consultation and education services that enable our users to take full advantage of our capabilities in single cell analytics and cell sorting. FLOW staff members consult with users to help them optimize assay design, data acquisition, data analysis, and data presentation. We provide guidance on instrument selection and operation. We inform users on incorporating appropriate technical controls into their experiments. FLOW offers regularly scheduled didactic courses and hands-on training to our users to advance their knowledge of flow cytometry and enhance their ability to perform high quality research at the lowest possible cost. FLOW organizes seminars, workshops, and vendor presentations that expand the knowledge base of our users. Support from the CCSG will enable us to continue to provide high level, cost-effective services to UWCCC members and thereby advance the UWCCC research mission.

项目摘要 /摘要 UWCCC于1988年建立了流式细胞仪实验室（流），以提供最新的 仪器和专业知识，使我们的成员能够在单一中获取和分析复杂的数据集 细胞和细胞群，并分离定义的细胞群以进行进一步研究。 在当前的CCSG资金中 周期，流量支持了137名独特的UWCCC成员的研究，代表了我们所有6个科学的研究 程序。 我们已经获得了新的工具，提高了我们的能力，建立了新服务，并且 扩大了我们针对UWCCC成员，他们的学员和员工的教育计划。我们订婚了 UWCCC领导者和我们的科学咨询委员会制定战略计划，该计划将使我们能够 继续在我们的任务中表现出色，并满足UWCCC成员迅速发展的研究需求。在 在下一个资金周期的过程中，我们将实现以下目标。特定目标1是提供UWCCC 成员可以访问用于单细​​胞分析和分类的最先进的仪器。流房， 操作并保持多参数分析细胞仪所需的各种仪器， 成像细胞仪和细胞分类。流已经建立了标准操作程序（SOP） 确保所有乐器都经过适当的校准，并以高质量的方式进行操作 达到实验性严格和可重复性的公认标准的数据集。具体目标2是 提供获得和分析多参数流式细胞术数据所需的专业知识。细胞分析 分类技术正在迅速发展，流动领导者监视这些进步，并努力 确保UWCCC成员能够在其研究中充分利用最新技术。流人员是 开发试剂面板的专家，使成员能够利用我们的工具收集多参数 具有最小光谱重叠的数据集。此外，Flow正在与我们的合作计划发展 生物统计学和癌症信息学共享资源，以确保生成多参数数据集 在我们的工具上，具有适当的统计和信息分析的能力。具体目标3是 提供专家咨询和教育服务，使我们的用户能够充分利用我们的 单细胞分析和细胞分选的功能。流动人员咨询用户以帮助他们 优化测定设计，数据采集，数据分析和数据显示。我们提供有关 仪器选择和操作。我们通知用户将适当的技术控制纳入他们的 实验。 Flow为我们的用户提供定期安排的教学课程和动手培训 他们对流式细胞术的了解并增强了他们以最低的方式进行高质量研究的能力 可能的成本。 Flow组织研讨会，研讨会和供应商演讲，以扩大知识 我们用户的基础。 CCSG的支持将使我们能够继续提供高水平，具有成本效益的 为UWCCC成员提供服务，从而推进UWCCC研究任务。