Special BD LSR Fortessa

特别 BD LSR Fortessa

• 批准号: 8640430
• 负责人: Manish S Patankar
• 金额: $ 35.05万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-01 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640430
• 关键词: Agreement; Allogenic; Antifungal Agents; Biomedical Research; Bone Marrow Transplantation; Cancer Center; Cardiac Myocytes; Cell Maturation; Cells; Child; Clinical Research; Clinical Trials; Cost Sharing; DNA Damage; Detection; Development; Ehlers-Danlos Syndrome; Farming environment; Flow Cytometry; Funding; Fungal Vaccines; Gene Mutation; Genetic Models; Grant; Hematologic Neoplasms; Hematopoietic stem cells; Human Biology; Human Pathology; Immune; Immune response; Immunotherapeutic agent; Inflammation; Institution; Kidney Diseases; Laboratories; Lasers; Maintenance; Malignant neoplasm of ovary; Mammary Neoplasms; Minor; Neurons; Price; Procedures; Prostate Cancer Vaccine; Quality Control; Rattus; Regulation; Research; Research Infrastructure; Research Personnel; Resolution; Sampling; Schedule; Services; System; Time; Training Support; Transplant Recipients; Tumor Suppression; Universities; Wages; Wheezing; Wisconsin; biobehavior; cost; experience; fibrogenesis; graft vs host disease; immune function; instrument; instrumentation; melanoma; member; mouse model; novel; programs; repaired; screening; transcription factor; treatment strategy; zinc-binding protein

DESCRIPTION (provided by applicant): The University of Wisconsin Carbone Cancer Center (UWCCC) Flow Cytometry Laboratory (Flow Lab) is a heavily utilized shared service on the University of Wisconsin-Madison campus available to researchers who are members as well as non-members of the UWCCC. The impact of the Flow Lab on biomedical research infrastructure at UW-Madison is significant. This Shared Instrumentation Grant is a request for funds that cover 80% (remaining 20% supported by the institution) of the total cost of a BD LSR Fortessa" (Fortessa) instrument to further enhance the capability and the capacity of the Flow Lab. The addition of the Fortessa will alleviate the 2-3 week wait times currently experienced by our users allowing timely analysis of clinical research samples. It will provide high laser power for increased resolution of cellular subpopulations and excitation wavelengths (561nm and 355nm) necessary for detection of newly developed fluorescent markers, and will include a High-Throughput Sampler option for increased efficiency and screening capability. This instrument will provide redundancy when the existing 5-laser LSR is undergoing maintenance and/or repair. The experienced staff, well-established daily Quality Control Procedures, Online Scheduling System and Educational Programs allow for seamless integration of the Fortessa into the Flow Lab. The Fortessa cytometer will impact the development of novel treatment strategies for various human pathologies and increase our understanding of human biology. Funded projects by major and minor users benefiting by addition of the Fortessa include (i) clinical trials for development of a prostate cancer vaccine (Doug McNeel), (ii) immunotherapeutic agents for melanoma (Paul Sondel), (iii) mouse models for CMML (Jing Zhang), (iv) rat models for genetic modulation of mammary cancer (Michael Gould), (v) modulating immune responses in ovarian cancer (Manish Patankar), (vi) Graft versus Host disease in allogeneic bone marrow transplant patients with hematologic malignancies, (vii) effect of biobehavioral factors on immune function (Erin Costanzo), (viii) fibrogenesis in kidney disease (Arjung Djamali), (ix) hematopoietic stem cell maturation into cardiomyocytes (Sean Palecek), (x) ventilatory control due to effects of inflammation on neuronal cells (Jyoti Watters), (xi) effect of genetic mutations in a zinc transporter on sphodylocheiro dysplastic form of Ehlers- Danlos Syndrome (David Eide), (xii) immune maturation and regulation in farm and non-farm raised children to better understand wheezing illnesses (David Gern), (xiii) transcription factors in tumor suppression and DNA damage (Randall Tibbets), and (xiv) development of anti-fungal vaccines (Marcel W¿thrich and Bruce Klein). Excellent institutional support in the form of 20% cost sharing of the total price of the Fortessa, salary support for trained technical staff, and the service agreement to maintain a fully functional and well-equipped cytometer will allow us to fulfill the research needs of major and minor users for the next 10 years.

描述（由应用程序提供）：威斯康星大学碳纤维癌症中心（UWCCC）流式细胞仪实验室（Flow Lab）是威斯康星大学麦迪逊分校校园的大量使用，可用于UWCCC的成员以及非成员的研究人员。流程实验室对UW麦迪逊分校生物医学研究基础设施的影响非常重要。这种共享的仪器赠款是覆盖BD LSR Fortessa总成本的80％（剩余20％的剩余20％（剩余的20％））的要求。兴奋的波长（561nm和355nm）用于检测新开发的荧光标记，并包括提高效率和筛查能力的高通量采样器选项，当现有的5-LASER LSR将提供冗余的效率。 Fortessa进入流实验室。 Fortessa细胞仪将影响各种人类病理的新型治疗策略的发展，并增加我们对人类生物学的理解。 Funded projects by major and minor users benefiting by addition of the Fortessa include (i) clinical trials for development of a prostate cancer vaccine (Doug McNeel), (ii) immunotherapeutic agents for melanoma (Paul Sondel), (iii) mouse models for CMML (Jing Zhang), (iv) rat models for genetic modulation of mammary cancer (Michael Gould), (v) modulating immunoresponses在卵巢癌（Manish patankar）中，（VI）移植物与宿主疾病在同种异体骨髓移植患者中具有血液学恶性肿瘤的患者，生物行为因素对免疫促进功能（Erin Costanzo）（Erin Costanzo）的影响（VII）对小子病中（VIII）的纤维化（VIII）（arjung djamali）的影响（I） into cardiomyocytes (Sean Palesek), (x) ventilation control due to effects of inflammation on neuronal cells (Jyoti Watters), (xi) effect of genetic mutations in a zinc transporter on sphodyloceiro dysplastic form of Ehlers- Danlos Syndrome (David Eide), (xii) immuno maturation and regulation in farm and non-farm raised children to更好地了解肿瘤疾病（David Gern），（XIII）肿瘤抑制和DNA损伤中的转录因子（Randall Tibbets）以及（XIV）抗真菌疫苗（Marcel Which和Bruce Klein）的开发。出色的机构支持，形式为20％的成本分配总价 Fortessa，对训练有素的技术人员的薪水支持以及维护功能齐全且设备齐全的细胞仪的服务协议将使我们能够在未来10年内满足主要和未成年人的研究需求。

项目成果

Short-term Immunopathological Changes Associated with Pulse Steroids/IVIG/Rituximab Therapy in Late Kidney Allograft Antibody Mediated Rejection.

• DOI: 10.34067/kid.0001082019
• 发表时间: 2020-05
• 期刊: Kidney360
• 作者: Degner KR;Wilson NA;Reese SR;Parajuli S;Aziz F;Garg N;Mohamed M;Singh T;Mandelbrot DA;Panzer SE;Redfield RR;Van Hyfte K;Zhong W;Hidalgo LG;Djamali A
• 通讯作者: Djamali A

Autologous Mesenchymal Stromal Cells Prevent Transfusion-elicited Sensitization and Upregulate Transitional and Regulatory B Cells.

• DOI: 10.1097/txd.0000000000000827
• 发表时间: 2018-09
• 期刊: Transplantation direct
• 影响因子: 2.3
• 作者: Zhang Z;Wilson NA;Chinnadurai R;Panzer SE;Redfield RR 3rd;Reese SR;Galipeau J;Djamali A
• 通讯作者: Djamali A