Flow Cytometry Laboratory

流式细胞术实验室

基本信息

批准号：
10626518
负责人：
Manish S Patankar
金额：
$ 3.94万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-04-25 至 2028-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10626518
关键词：
Advisory Committees Antibodies Award Basic Science Biological Biological Assay Biometry Biotechnology Businesses Calibration Cancer Center Cancer Center Support Grant Canis familiaris Cell Separation Cells Clinical Clinical Research Collaborations Color Communities Comprehensive Cancer Center Computer software Computers Consultations Cytometry DNA Data Data Analyses Data Set Dedications Development Education Educational workshop Faculty Family suidae Flow Cytometry Flow Cytometry Shared Resource Fluorescence Funding Genomics Goals Grant Human Image Immunology Immunotherapy Infrastructure Institution Instruction Intervention Journals Label Laboratories Lasers Leadership Location Malignant Childhood Neoplasm Malignant Neoplasms Malignant neoplasm of ovary Manufacturer Marketing Measures Medical Research Methods Mission Modernization Pediatric Neoplasm Peer Review Play Population Productivity Protocols documentation Publications Publishing RNA Radiation therapy Radionuclide therapy Reagent Reproducibility Research Research Personnel Research Project Grants Resource Sharing Resources Rodent Role Sampling Services Sorting Strategic Planning Surveys Technical Expertise Training Training and Education Translational Research United States National Institutes of Health Universities Wisconsin animal imaging assay development cellular imaging complex data computerized tools cost data acquisition design equipment acquisition experience experimental study informatics shared resource instrument instrumentation laboratory facility lectures member new technology next generation sequencing operation pediatric department peer precision medicine programs protocol development radioactivity analysis satisfaction single cell analysis single cell sequencing theranostics

项目摘要

PROJECT SUMMARY / ABSTRACT Since its inception in 1988, the Flow Cytometry Laboratory (FLOW) has served as the only comprehensive shared resource on the UW-Madison campus for multiparameter flow cytometry, cell sorting and imaging cell cytometry. The mission of FLOW is to operate a cutting-edge facility that provides UWCCC researchers the infrastructure, technical expertise, and educational support required for fluorescence-based single cell analysis and isolation that advances basic, translational, and clinical research. FLOW manages one 67-parameter Cytek Aurora, five 16-parameter ThermoFisher Attune, one 20-parameter BD LSRII, and one 20-parameter BD LSR Fortessa analytical cytometers; three BD FACSAria and one Sony MA900 cell sorters; and a Luminex ImageStreamX MarkII imaging cytometer. Strong institutional support and funds from NIH S10 Shared Instrumentation Grants have allowed FLOW to purchase and maintain state-of-the-art instruments, computers, and software needed for data acquisition and analysis. FLOW operates facilities at two locations: 1) our main laboratory in the Wisconsin Institute for Medical Research (WIMR), which is located on West Campus and houses the majority of UWCCC researchers; and 2) a satellite facility in the UW Biotechnology Center (UWBC), which is located on Central Campus near our remaining members and contiguous to facilities that provide DNA, RNA, and single-cell next-generation sequencing to characterize freshly sorted single cells and cell populations. FLOW partners with the Department of Pediatrics and the UWCCC Small Animal Imaging and Radiotherapy Facility (SAIRF) to operate cytometers dedicated primarily to studies of pediatric cancers and analysis of radioactive samples, respectively. The latter partnership was established to support targeted radionuclide therapy of pediatric tumors in combination with immunotherapy. Dr. Manish Patankar (TM), a UWCCC member and ovarian cancer researcher, serves as faculty leader. Ms. Dagna Sheerar has managed day-to-day operations since 2011. Ms. Sheerar and her 6 staff members possess over 80 years of collective experience in cytometry. The specific aims for FLOW are to: 1) evaluate new technologies and develop business plans to procure and maintain new instruments allowing users access to cutting-edge approaches to single cell analytics and sorting; 2) provide expert consultation and education services for single cell analytics and cell sorting ; and 3) organize user groups and develop data analysis support in collaboration with the Cancer Informatics Shared Resource for the increasingly complex data sets generated by multi-parameter cytometers and sorters. During the current CCSG funding cycle, FLOW served 109 unique UWCCC program members across all six programs who collectively hold/held 445 cancer relevant research grants, including 118 awards from the NCI, and contributed to 169 cancer relevant UWCCC member publications. Survey data indicate a high level of user satisfaction and project increasing demand for services offered by FLOW.

项目概要/摘要自 1988 年成立以来，流式细胞术实验室 (FLOW) 一直是唯一的综合性实验室威斯康辛大学麦迪逊分校校园的多参数流式细胞术、细胞分选和细胞成像共享资源细胞计数术。 FLOW 的使命是运营一个尖端设施，为 UWCCC 研究人员提供基于荧光的单细胞分析所需的基础设施、技术专业知识和教育支持和隔离促进基础、转化和临床研究。 FLOW 管理一台 67 参数 Cytek Aurora、5 个 16 参数 ThermoFisher Attune、1 个 20 参数 BD LSRII 和 1 个 20 参数 BD LSR Fortessa 分析细胞仪；三台 BD FACSAria 和一台 Sony MA900 细胞分选仪；和一个 Luminex ImageStreamX MarkII 成像细胞仪。来自 NIH S10 的强大机构支持和资金共享仪器补助金允许福禄购买和维护最先进的仪器、计算机、以及数据采集和分析所需的软件。 FLOW 在两个地点运营设施：1) 我们的主要威斯康星医学研究所 (WIMR) 的实验室位于西校区 UWCCC 的大部分研究人员都住在这里； 2) 威斯康星大学生物技术中心 (UWBC) 的卫星设施，它位于中央校区，靠近我们剩余的成员，并且毗邻提供 DNA 的设施， RNA 和单细胞下一代测序可表征新分选的单细胞和细胞群。 FLOW 与儿科和 UWCCC 小动物成像和放射治疗部合作操作细胞仪的设施（SAIRF）主要用于儿科癌症的研究和分析分别为放射性样品。后一个伙伴关系的建立是为了支持目标放射性核素与免疫疗法相结合的儿童肿瘤治疗。 Manish Patankar 博士 (TM)，UWCCC 会员和卵巢癌研究员，担任教员领导。 Dagna Sheerar 女士负责管理日常事务自 2011 年开始运营。Sheerar 女士和她的 6 名员工在以下方面拥有 80 多年的集体经验细胞计数术。 FLOW 的具体目标是： 1) 评估新技术并制定业务计划采购和维护新仪器，使用户能够获得单细胞分析的尖端方法和排序； 2) 提供单细胞分析和细胞分选方面的专家咨询和教育服务；和 3）与癌症信息学共享组织合作组织用户组并开发数据分析支持多参数细胞仪和分选仪生成的日益复杂的数据集的资源。期间在当前的 CCSG 资助周期中，FLOW 为所有六个项目中的 109 名独特的 UWCCC 项目成员提供了服务他们总共拥有 445 项癌症相关研究资助，其中包括 118 项来自 NCI 的奖项，以及为 169 篇癌症相关的 UWCCC 成员出版物做出了贡献。调查数据显示用户水平较高满意度并预计对福禄提供的服务的需求不断增加。