Development of A Novel Magnetic Exosomal Assay for Diagnosis of Diseases with Impaired Vascular Integrity

开发一种用于诊断血管完整性受损疾病的新型磁性外泌体测定法

• 批准号: 9410664
• 负责人: Peng Liu
• 金额: $ 5万
• 依托单位: MICROSENSOR LABS, LLC
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-03-15 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9410664
• 关键词: Acute Lung Injury; Affect; Antibodies; Atherosclerosis; Biological; Biological Assay; Biological Markers; Biological Sciences; Blood Vessels; Body Fluids; Cause of Death; Cell Communication; Cells; Clinical; Communities; Complex; Consumption; Data; Defect; Detection; Development; Diabetes Mellitus; Diagnosis; Diagnostic; Diagnostic tests; Disadvantaged; Disease; Dose; Elderly; Electromagnetics; Electronics; Elements; Endothelial Cells; Equipment; Ethnic Origin; Event; Goals; Human; Impairment; Intercellular Junctions; Ischemia; Liquid substance; Magnetism; Maintenance; Messenger RNA; MicroRNAs; Neoplasm Metastasis; Noise; Optics; Patients; Phase; Pneumonia; Population; Process; Proteins; RNA; Race; Reader; Reperfusion Therapy; Reproducibility; Research; Research Proposals; Resolution; Running; Sampling; Sensitivity and Specificity; Sepsis; Serum; Signal Transduction; Small Business Innovation Research Grant; System; Therapeutic; Time; Transportation; Tumor Angiogenesis; United States; Woman; base; cost; design; disease diagnosis; exosome; extracellular vesicles; liquid biopsy; lung injury; magnetic beads; next generation; novel; novel marker; outcome forecast; public health relevance; response; sensor; tool

 DESCRIPTION (provided by applicant): This Small Business Innovation Research Phase 1 project aims to develop and characterize a novel Magnetic Exosomal Assay to isolate and detect single intact exosome concentrations in human serum. This proposed research will also evaluate the diagnostic potential of the Magnetic Exosomal Assay for diseases with impaired vascular integrity. Vascular integrity or the maintenance of blood vessel continuity is a fundamental process maintained through endothelial cell-cell junctions. Defects in endothelial barrier function are an initiating factor in several disease processes including atherosclerosis, ischemia/reperfusion, tumor angiogenesis, cancer metastasis, diabetes, sepsis and acute lung injury. An initial event in impaired vascular integrity is a dramatic increase in a novel type of extracellular vesicle (EV) from endothelial cells (EC) called exosomes. Therefore, the development of a highly sensitive, reproducible and easy-to- implement assay to detect exosome concentrations in complex clinical samples can have potential diagnostic and therapeutic benefit. In this research proposal, we will develop and characterize a novel Magnetic Exosomal Assay kit and reader for fast isolation and detection of single intact exosome concentrations in human serum. In addition, we will assess the optimized Magnetic Exosomal Assay on Community-Acquired Pneumonia patient serum. The ultimate goal of this research is to develop a highly sensitive, reproducible and easy to implement exosomal assay as a diagnostic tool for patients with various types of impaired vascular integrity-related diseases.

 描述（由申请人提供）：该小型企业创新研究第一阶段项目旨在开发和表征一种新型磁性外泌体测定，以分离和检测人血清中的单个完整外泌体浓度。这项拟议的研究还将评估磁性外泌体的诊断潜力。血管完整性受损的疾病的测定或血管连续性的维持是通过内皮细胞-细胞连接维持的基本过程。血管完整性受损的初始事件是内皮细胞中新型细胞外囊泡 (EV) 的急剧增加。因此，开发一种高度灵敏、可重复且易于实施的检测方法来检测复杂临床样本中的外泌体浓度可能具有潜在的诊断和治疗作用。在本研究提案中，我们将开发并表征一种新型磁性外泌体测定试剂盒和读取器，用于快速分离和检测人血清中的单个完整外泌体浓度。此外，我们还将评估针对社区获得性肺炎的优化磁性外泌体测定。这项研究的最终目标是开发一种高度敏感、可重复且易于实施的外泌体检测方法，作为患有各种类型的血管完整性受损相关疾病的患者的诊断工具。