SBIR Fast Track: Development of a High-throughput Magnetic Cytometer for Single Cell Sorting

SBIR 快速通道：开发用于单细胞分选的高通量磁力细胞仪

基本信息

批准号：
10703514
负责人：
Peng Liu
金额：
$ 72.64万
依托单位：
MICROSENSOR LABS, LLC
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-04-01 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10703514
关键词：
Address Algorithms B-Lymphocytes Basic Science Biological Assay Biological Sciences Blood Blood Cells Blood specimen Budgets Businesses Cell Culture Techniques Cell Line Cell Separation Cell Survival Cells Cellular Structures Clinical Collaborations Complex Computer software Consumption Dedications Detection Development Devices Goals Healthcare Hour Innovation Corps Interview Legal patent Magnetism Marketing Mechanics Microfluidics Micromanipulation Molecular Analysis Movement National Institute of General Medical Sciences Neoplasm Circulating Cells Optics Patients Phase Population Population Heterogeneity Protocols documentation Recovery Research Research Project Grants Resolution Sampling Semiconductors Small Business Innovation Research Grant Sorting Speed Stains System Techniques Technology Time Translational Research United States National Institutes of Health Validation antigen-specific T cells cell fixation clinical application commercialization cost effective design fetus cell fluorescence imaging imaging system innovation instrumentation interest microsensor monoclonal antibody production next generation novel optical imaging particle precision medicine processing speed protocol development prototype rapid growth sample fixation sensor single cell analysis single-cell RNA sequencing software development stem cells technology platform tool tumor initiation user-friendly waiver

项目摘要

Project Summary This Small Business Innovation Research project aims to develop a high-throughput easy-to-use cell sorter MagiCyte™ to rapidly and precisely sort intact and viable rare cells at single-cell resolution. Single-cell sorting from a heterogeneous cell population has been essential to life science applications such as monoclonal antibody production and cell line development. Recently, with the rapid growth of precision medicine, there has also been a strong demand for single-cell sorting technologies for rare cells, such as circulating tumor cells (CTCs), circulating fetal cells, stem cells and antigen-specific T- or B-cells. However, collecting such rare cells at single-cell resolution to meet the requirements of single-cell analysis is not feasible for conventional single-cell sorting techniques. Micromanipulation and serial dilution are time consuming and low-throughput, while fluorescent-activated cell sorting (FACS) suffers from remarkable cell loss and is inefficient in isolating rare cells. In addition, many such techniques require cell fixation and/or fluorescent staining, which decreases cell viability and thus prohibits many downstream molecular analyses, as well as and cell culture. As such, there is an unaddressed need for high throughput single-cell sorting of rare cells. We aim to develop a high-throughput chip-based cytometer MagiCyte™ to rapidly isolate, identify and sort single intact and viable target cells from heterogeneous cell population. The core component of MagiCyte is a semiconductor chip MagiChip™ which is used for single cell identification and single cell sorting. In this fast- track project, we will first develop the control software and demonstrate automated single-cell sorting; In Phase II, we will develop the next-generation MagiChip, build the MagiCyte beta product and validate the system on clinical samples with our collaborators.

项目概要该小型企业创新研究项目旨在开发一种高通量、易于使用的细胞分选仪 MagiCyte™ 以单细胞分辨率快速、精确地分选完整且存活的稀有细胞。从异质细胞群中进行单细胞分选对于生命科学应用至关重要，例如近年来，随着精准度的快速增长，单克隆抗体生产和细胞系开发。医学方面，对稀有细胞的单细胞分选技术也有强烈的需求，例如循环肿瘤细胞 (CTC)、循环胎儿细胞、干细胞和抗原特异性 T 细胞或 B 细胞。以单细胞分辨率收集如此稀有的细胞来满足单细胞分析的要求是不可行的对于传统的单细胞分选技术来说，显微操作和连续稀释非常耗时且耗时。通量低，而荧光激活细胞分选 (FACS) 会遭受显着的细胞损失，并且此外，许多此类技术需要细胞固定和/或荧光。染色，会降低细胞活力，从而阻止许多下游分子分析，以及因此，对稀有细胞的高通量单细胞分选的需求尚未得到解决。我们的目标是开发一种基于高通量芯片的细胞仪 MagiCyte™，以快速分离、识别和分类来自异质细胞群的单个完整且可行的靶细胞 MagiCyte 的核心组件是半导体芯片 MagiChip™ 用于单细胞识别和单细胞分选。跟踪项目，我们将首先开发控制软件并演示自动化单细胞分选； II，我们将开发下一代MagiChip，构建MagiCyte beta产品并在其上验证系统与我们的合作者一起提供临床样本。