Resource Core

资源核心

• 批准号: 9211066
• 负责人: OLIVIER LE SAUX
• 金额: $ 66.25万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9211066
• 关键词: Address; Affect; Alpha Cell; Animal Model; Animals; Bioinformatics; Biomedical Research; Caucasians; Cell model; Centers of Research Excellence; Clinical; Communities; Data Analyses; Data Quality; Development; Diabetes Mellitus; Diagnosis; Disease; Education; Ensure; Environment; Epidemic; Epigenetic Process; Fee-for-Service Plans; Filipino; Gene Expression; Genes; Genetic Transcription; Genetic Variation; Genome; Genomic DNA; Genomics; Genus Hippocampus; Goals; Grant; Hawaii; Hawaiian population; Health; Heritability; Hyperglycemia; In Vitro; Instruction; International; Ions; Island; Japanese Population; Libraries; Mentors; Metabolic; Metabolism; Minority; Modernization; Mutation; Pacific Island Americans; Population; Population Group; Prevention; Recovery; Research; Research Infrastructure; Research Personnel; Resources; Services; Site; Technology; Training; Training and Education; Universities; Variant; Work; biomedical resource; clinical Diagnosis; cost; design; epigenomics; health disparity; improved; in vivo; instrumentation; interest; metabolic abnormality assessment; new technology; next generation; transcriptome sequencing

Abstract: The overall objective of the Resource Core is to provide an easily accessible, modern, and local set of expertise in cellular and animal metabolism and the epigenetics associated with diabetes to ultimately provide a sustainable resource for the biomedical research community to assess cellular and animal metabolics along with the genetic changes associated with a clinical diagnosis. The central hypothesis for this Resource Core is that the core will provide an unprecedented set of expertise, education, and training by establishing the infrastructure necessary to investigate the mechanisms, diagnosis, prevention, and/or treatment of diabetes in a multi-faceted approach to improve health and address health disparities in minority populations in Hawaii. The following aims will support the goals of the Resource Core to achieve the overall objective. Specific Aim 1: Establish a cell and animal metabolic facility for the growing number of investigators interested in studying diabetes. We plan to enhance cellular metabolic instrumentation and install animal metabolic infrastructure to study diabetes. We will provide services in, in-vitro to in-vivo metabolic studies including the design, development, optimization, and delivery as requested, as we have extensive expertise in cellular metabolism and animal models. Specific Aim 2: Establish an epigenetics facility to evaluate heritable variations and transcriptional changes in genes associated with diabetes. We have extensive expertise in gene expression studies to develop and deliver a wide-array of epigenomic services. We plan to enhance the epigenetics core by providing expertise and ground-breaking technology like MeDIP-, ChIP-, gDNA-, Ampli-, and RNA- sequencing and bioinformatics data analysis. This technology will be accessible to interested investigators. Specific Aim 3: Provide education and instruction in the resources and capabilities of the Core. Cores are critical to produce high quality data but also to the training and development of “quality” investigators. The advantage of a core is the consistency and quality of the work performed. Specifically, this core will include training and facilitation of use to assess cellular metabolism (Seahorse XFe96), animal metabolism (GTT, ITT, hyperglycemic and hyperinsulinemic-euglycemic), and genetic variation (Ion Personal Genome Machine®). We plan to integrate a fee for service/cost recovery/sharing plan to ensure that the core is self-sustaining while integrating new technologies in the field.

抽象的： 资源核心的总体目标是提供易于访问，现代和本地的一组 细胞和动物代谢以及与糖尿病相关的表观遗传学方面的专业知识，最终提供 生物医学研究界评估细胞和动物代谢的可持续资源 与临床诊断相关的遗传变化。此资源核心的中心假设 是核心将通过建立一套空前的专业知识，教育和培训集 研究糖尿病的机制，诊断，预防和/或治疗所需的基础设施 一种多方面的方法来改善夏威夷少数族裔人口的健康差异。 以下目标将支持资源核心的目标以实现总体目标。 特定目的1：为越来越多的研究者建立一个细胞和动物代谢设施 有兴趣研究糖尿病。 我们计划增强细胞代谢仪器并安装动物代谢基础设施进行研究 糖尿病。我们将在体内的体内代谢研究中提供服务，包括设计，开发， 根据要求的优化和交付，因为我们在细胞代谢和动物方面具有广泛的专业知识 型号。 特定目的2：建立一个表观遗传学设施来评估可遗传的变化和转录 与糖尿病相关的基因的变化。 我们在基因表达研究方面拥有广泛的专业知识，以发展和提供广泛的表观基因组学 服务。我们计划通过提供专业知识和开创性技术来增强表观遗传学核心 Medip-，chip-，gdna-，Ampli-和RNA测序和生物信息学数据分析。这项技术将 有趣的调查员可以使用。 特定目的3：提供核心资源和能力的教育和指示。 核心对于产生高质量数据至关重要，也对培训和开发“质量”至关重要 调查人员。核心的优点是执行工作的一致性和质量。具体来说， 该核心将包括训练和准备用于评估细胞代谢的用途（Seahorse XFE96）， 动物代谢（GTT，ITT，高血糖和高胰岛素血糖）和遗传变异 （离子个人基因组机®）。我们计划将服务/成本恢复/共享计划的费用整合到 确保核心在将新技术集成到现场时是自我维持的。