EXAMINATION OF CHOLESTEROL DISPOSITION IN VIVO IN HUMANS

人体体内胆固醇分布的检查

• 批准号: 6665927
• 负责人: RICHARD E. OSTLUND
• 金额: $ 15.75万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6665927
• 关键词: aging; biological products; biomedical resource; clinical research; drug /agent; endocrine gland /system; female; hormones; human subject; male; musculoskeletal system; physical fitness; proteins; spectrometry; women's health

An elevated plasma low density lipoprotein cholesterol level is an important risk factor for atherosclerotic cardiovascular disease, and excessive dietary cholesterol intake may contribute to such elevations. Dietary cholesterol must be absorbed from the intestine to reach the systemic circulation, but factors that govern cholesterol absorption in humans are poorly understood. Our studies examine absorption and metabolism of cholesterol, oxysterols, and phytosterols in human subjects using stable isotope tracers and negative ion electron capture (NIEC) mass spectrometry (MS). Such methods avoid radioactive exposure to subjects and achieve accurate measurement and certain identification of target analytes. GC/NIEC/MS provides an exquisitely sensitive and structurally specific tool for analyses of fluorinated derivatives of sterols employed in our studies. Development of these methods, including selection and preparation of appropriate derivatives and characterization of synth eticcholesterol tracers labeled with multiple stable isotope atoms, are important innovations that were supported by the Washington University Mass Spectrometry Resource. Descriptions of these methods are occuring at several national meetings each year.

血浆低密度脂蛋白胆固醇水平升高是 动脉粥样硬化性心血管疾病的重要危险因素，以及 膳食胆固醇摄入过多可能会导致这种情况 海拔。 膳食胆固醇必须从肠道吸收 到达体循环，但控制胆固醇的因素 对人类的吸收知之甚少。 我们的研究考察 胆固醇、氧甾醇和植物甾醇的吸收和代谢 在人类受试者中使用稳定同位素示踪剂和负离子 电子捕获 (NIEC) 质谱 (MS)。 此类方法避免 受试者的放射性暴露并实现精确测量和 目标分析物的某些识别。 GC/NIEC/MS 提供了 极其灵敏且结构特定的工具，用于分析 我们的研究中使用的甾醇的氟化衍生物。 这些方法的开发，包括选择和制备 适当的导数和表征 用多种稳定同位素标记的合成乙胆甾醇示踪剂 原子，是华盛顿支持的重要创新 大学质谱资源。 这些方法的说明 每年都会在几次全国会议上进行。