PLASMA GLUTAMINE & LEUCINE RATES OF APPEARANCE IN AIDS ASSOC MUSCLE WASTING

血浆谷氨酰胺

• 批准号: 6665905
• 负责人: KE YARASHESKI
• 金额: $ 15.75万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6665905
• 关键词: biomedical resource; carbohydrates; diabetes mellitus; endocrine gland /system; female; hormones; human subject; male; spectrometry; technology /technique

Muscle protein wasting occurs in human immunodeficiency virus (HIV)-infected individuals and is often the initial indication of acquired immunodeficiency syndrome (AIDS). The alterations in muscle protein metabolism that occur with HIV-infection are unknown. Seven subjects with AIDS-wasting (CD4<200/mm3), chronic stable opportunistic infections (OI) and >10% weight loss, 10 HIV-infected men and 1 woman (CD4>200/mm3) without wasting or OI (asymptomatic), and 6 HIV-negative lean men (control) received a constant intravenous infusion of 1-[13C]-leucine and 2-[15N]-glutamine. Plasma leucine and glutamine rate of appearance (Ra), whole-body leucine turnover, disposal and oxidation rates, and 13C-leucine incorporation rate into mixed muscle protein were assessed. Fasting whole-body proteolysis and synthesis rates were increased (P<0.05) above control (129 and 96 (mol/kg FFM/h) in the asymptomatic HIV-infected subjects (145 and 110 (mol/kg FFM/h), and further increased (P< 0.05) in A IDS-wasting subjects (153 and 123 (mol/kg FFM/h). Fasting mixed muscle protein synthesis rate was increased in the asymptomatic subjects (0.062 %/h; P<0.05), but similar in AIDS-wasting and control subjects (0.036 vs 0.037 %/h). Plasma glutamine Ra was increased (P<0.001) in AIDS-wasting subjects (528 (mol/kg FFM/h), but similar in control and asymptomatic subjects (408 and 390 (mol/kg FFM/h). These findings suggest that AIDS-wasting results from; (a) a preferential reduction in muscle protein, (b) a failure to sustain an elevated rate of mixed muscle protein synthesis while whole-body protein turnover is increased, and (c) a significant increase in glutamine release into the circulation, probably from muscle. Several interesting possibilities exist for the increased glutamine Ra in AIDS-wasting.

人类免疫缺陷病毒会导致肌肉蛋白浪费 (HIV) 感染者，通常是以下症状的最初迹象： 获得性免疫缺陷综合症（艾滋病）。 肌肉的变化 HIV 感染时发生的蛋白质代谢尚不清楚。 七 艾滋病消耗者（CD4<200/mm3），慢性稳定机会性 感染 (OI) 和体重减轻 >10%，10 名 HIV 感染男性和 1 名女性 (CD4>200/mm3)，无消瘦或成骨不全（无症状），且 6 名 HIV 阴性 瘦男性（对照）接受持续静脉输注 1-[13C]-亮氨酸和2-[15N]-谷氨酰胺。 血浆亮氨酸和谷氨酰胺 出现率 (Ra)、全身亮氨酸周转、处置和 氧化速率和 13C-亮氨酸掺入混合肌肉的速率 蛋白质进行了评估。 空腹全身蛋白水解和合成 比率比对照增加（P<0.05）（129 和 96（mol/kg FFM/h） 在无症状的 HIV 感染者中（145 和 110 (mol/kg FFM/h)， 并且在 A IDS 消耗受试者中进一步增加（P < 0.05）（153 和 123） （摩尔/千克 FFM/小时）。 空腹混合肌蛋白合成率为 无症状受试者中增加（0.062 %/h；P<0.05），但 艾滋病消耗者和对照受试者的情况相似（0.036 %/h vs 0.037 %/h）。 艾滋病消瘦受试者血浆谷氨酰胺 Ra 升高（P<0.001） （528（mol/kg FFM/h），但在对照和无症状受试者中相似 （408 和 390（mol/kg FFM/h）。这些发现表明艾滋病消耗 结果来自； (a) 优先减少肌肉蛋白，(b) 未能维持混合肌肉蛋白质合成率的升高 同时全身蛋白质周转率增加，并且（c）显着 谷氨酰胺释放到循环中的增加，可能来自 肌肉。 增加的数量存在一些有趣的可能性 艾滋病消耗中的谷氨酰胺Ra。