Microscopy and Flow Cytometry Core

显微镜和流式细胞术核心

基本信息

批准号：
9794383
负责人：
Chang-UK Lim
金额：
$ 22.35万
依托单位：
UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至 2024-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9794383
关键词：
3-Dimensional Affect Air Anatomy Animal Model Animal Organ Animals Antibodies Apoptosis Applications Grants Biological Carbon Dioxide Cell Aging Cell Count Cell Cycle Progression Cell Death Cell Separation Cells Centers of Research Excellence Chemicals Color Computer software Core Facility Cryoultramicrotomy Data Analyses Diagnosis Equipment Flow Cytometry Fluorescence Fluorescence Resonance Energy Transfer Genetic Histopathology Human Human Pathology Human Resources Image Image Analysis Immunofluorescence Immunologic Immunohistochemistry In Vitro Ions Laboratories Lasers Lesion Light Manuscripts Measurement Metabolism Microscope Microscopic Microscopy Modification Molecular Target Morphology Paraffin Pathologic Pathologist Pathology Pathology Report Pharmaceutical Preparations Phase Preparation Protocols documentation Reagent Research Design Research Personnel Research Project Grants Resources Running Scanning Services Sorting - Cell Movement South Carolina Specimen Handling Speed Stains Sterility Techniques Temperature Time Tissue Sample Tissues Training Universities Video Microscopy animal tissue cell growth chemical genetics design experience experimental study flexibility fluorescence imaging fluorescence microscope human tissue in vivo instrument instrumentation live cell imaging medical schools microsystems programs ratiometric reconstruction small molecule success targeted treatment tumor vibration

项目摘要

ABSTRACT The Microscopy and Flow Cytometry Core (MFCC) of the COBRE Center for Targeted Therapeutics (CTT) provides services to all four CTT research projects and other investigators supported by the CTT and other IDeA programs at the University of South Carolina (USC). These services include training in microscopy and flow cytometry, advice on study design, with an emphasis on the analysis of cellular drug effects, and assistance with the instrumentation usage, both at the Core and at other USC facilities (in particular at the USC School of Medicine), as well as expert assistance in anatomic pathology of human and animal tissues. The available equipment includes: Carl Zeiss LSM700 Laser scanning confocal microscope with 4 lasers functioning from 405 to 633 nm, equipped for live cell imaging (with environmental chamber), standard point scanning, 3D reconstruction, FRAP, FRET, time lapse and tile/stitching; a Leica Microsystems AS MDW fluorescence microscope optimized for live cell imaging with temperature and CO2 control; two Leica Microsystems DM IRE2 fluorescent microscopes, which are motorized for z-stack acquisition and one of which has an environmental chamber. Olympus IX81 fluorescence microscope with workstation running Metamorph software is used for fluorescence imaging and deconvolution of wide field fluorescence images; it also has a Sutter Instruments Smartshutter and rapid filter changing wheels for ratiometric measurement of intracellular ion changes. There are 3 Carl Zeiss Axiovert 200 microscopes and an Olympus upright BX41 microscope, each with a color camera. All microscopes are in dedicated rooms and on vibration isolation tables. Flow cytometric equipment includes the BD FACS ARIA III for cell sorting, BD LSR II Flow Cytometer with high-throughput option and a 96-well plate loader, and Beckman Coulter FC500 flow cytometer. Users may operate the microscopes and one of the flow cytometers independently, after training by the co-directors. Many standard protocols have been established for immunohistochemistry and immunofluorescence analysis of cells and tissues, and for the analysis of cell growth, cell death and cell cycle progression. The core also provides services of an experienced anatomic pathologist, with a broad experience in gross pathology and histopathology in human and animal studies. The pathology core services include advice on study design and specimen processing including paraffin and cryosectioning, routine H&E staining, histochemical stains, and immunohistochemical analysis. Pathology reports will include macroscopic and microscopic findings, diagnosis of lesions, discussion of significant findings, and biological significance of the pathological effects of genetic modifications and experimental compounds in different animal organs, as well as tumor morphology in animal models and in human tissue samples. The core personnel offer advice on the choice of instruments and experimental protocols, provide training and assist with preparation of manuscripts and grant proposals.

抽象的目标治疗中心（CTT）的显微镜和流式细胞仪核（MFCC）为CTT和其他想法支持的所有四个CTT研究项目和其他研究人员提供服务南卡罗来纳大学（USC）的计划。这些服务包括显微镜和流程的培训细胞仪，研究设计的建议，重点是分析细胞药物的作用，并有助于在核心和其他USC设施中，仪器使用量（尤其是在USC学校医学），以及人类和动物组织解剖病理学的专家辅助。可用设备包括：Carl Zeiss LSM700激光扫描共聚焦显微镜，405个激光器功能到633 nm，配备了活细胞成像（带有环境室），标准点扫描，3D 重建，FRAP，FRET，时间流失和瓷砖/缝合； Leica微型系统作为MDW荧光用于通过温度和二氧化碳对照的活细胞成像优化的显微镜；两个Leica Microsystems DM IRE2 荧光显微镜，该显微镜用于Z-stack采集，其中之一具有环境腔室。 Olympus IX81荧光显微镜，带有工作站运行Metamorph软件用于大田荧光图像的荧光成像和反卷积；它也有萨特乐器 SmartShutter和快速滤波器更换轮，用于对细胞内离子变化的比例测量。那里是3个Carl Zeiss Axiovert 200显微镜和一个Olympus直立BX41显微镜，每个显微镜都带有彩色摄像头。所有显微镜都在专用的房间和振动隔离表中。流式细胞仪设备包括 BD FACS ARIA III用于细胞分类，BD LSR II流式细胞仪具有高通量选项和96孔板装载机和Beckman Coulter FC500流式细胞仪。用户可以操作显微镜和流程之一由联合导演训练后，独立地细胞仪。已经建立了许多标准协议细胞和组织的免疫组织化学和免疫荧光分析，以及用于细胞生长的分析，细胞死亡和细胞周期进展。核心还提供经验丰富的解剖病理学家的服务，在人类和动物研究中具有广泛的病理学和组织病理学经验。病理核心服务包括有关研究设计和标本加工的建议，包括石蜡和冷冻，例行程序 H＆E染色，组织化学染色和免疫组织化学分析。病理报告将包括宏观和微观发现，病变的诊断，对重要发现的讨论以及生物学不同动物遗传修饰和实验化合物的病理作用的重要性器官以及动物模型和人体组织样品中的肿瘤形态。核心人员提供有关仪器选择和实验协议的建议，提供培训并协助准备手稿和授予建议。