ShEEP Request for Nikon N-Storm Super-resolution microscope

ShEEP 请求尼康 N-Storm 超分辨率显微镜

• 批准号: 9795345
• 负责人: Ann C. McKee
• 金额: --
• 依托单位: VA BOSTON HEALTH CARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9795345
• 关键词: 3-Dimensional; Aging; Alzheimer' s Disease; Area; Biological Markers; Biological Models; Boston; Cell model; Cells; Dementia; Dimensions; Disease; Ensure; Epilepsy; Fluorescent in Situ Hybridization; Funding; Genetic Transcription; Healthcare; Healthcare Systems; Image; Imaging Techniques; In Situ Hybridization; Individual; Lateral; Lighting; Messenger RNA; Methods; Microscope; Microscopy; Molecular; Nanoscopy; Nerve Degeneration; Neurodegenerative Disorders; Optics; Pathologic; Pathology; Phase; Positioning Attribute; Process; Production; Proteins; Research; Research Personnel; Research Project Grants; Research Support; Resolution; Sheep; Specimen; Speed; Structure; System; Techniques; Time; Tissue Sample; Tissues; Transcript; Traumatic Brain Injury; age related; chronic traumatic encephalopathy; experimental study; fluorescence microscope; improved; in vivo; instrumentation; microscopic imaging; molecular scale; nanometer; nanoscale; neuronal circuitry; pre-clinical; programs; reconstruction; two-dimensional

The VA Boston Healthcare System (VABHS) is requesting the purchase of a state-of-the-art Nikon N-Storm super-resolution microscope. This microscope can resolve objects the size of individual molecules. The system can be used to create images of pathological proteins in neurodegenerative diseases like chronic traumatic encephalopathy (CTE), Alzheimer’s disease (AD), and other aging-related neurodegenerative disorders. These images can be used to better understand how pathological molecules spread from cell to cell. It can also be used to study the cellular dynamics of epilepsy and traumatic brain injury in cellular model systems. In addition, new advances have made it possible to use the incredible resolution of the N-Storm microscope to quantify transcriptional expression of individual cells, thereby understand how neurodegenerative diseases alter transcriptional expression. Understanding what molecules are involved in neurodegeneration will provide biomarkers to identify diseases in the preclinical phases and ultimately develop better therapies for neurodegenerative disease. The capabilities of this system are not available within VABHS and are critical to ongoing research projects

VA波士顿医疗保健系统（VABHS）要求购买最先进的尼康 N态超分辨率显微镜。该显微镜可以解析单个大小的对象 分子。该系统可用于创建病理蛋白的图像 神经退行性疾病，如慢性创伤性脑病（CTE），阿尔茨海默氏病 （AD）和其他与衰老有关的神经退行性疾病。这些图像可用于更好 了解病理分子如何从细胞传播到细胞。它也可以用来研究 细胞模型系统中癫痫和创伤性脑损伤的细胞动力学。此外， 新的进步使使用N-STORM显微镜的令人难以置信的分辨率成为可能 为了量化单个细胞的转录表达，从而了解 神经退行性疾病改变转录表达。了解什么是分子 参与神经变性将提供生物标志物以鉴定临床前的疾病 阶段并最终为神经退行性疾病提供更好的疗法。功能 该系统的vabhs中不可用，对于正在进行的研究项目至关重要