Epigenetics, environmental exposure, and reproduction in the Collaborative Cross

协同杂交中的表观遗传学、环境暴露和繁殖

基本信息

批准号：
8831815
负责人：
David Lawrence Aylor
金额：
$ 24.9万
依托单位：
NORTH CAROLINA STATE UNIVERSITY RALEIGH
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-06-17 至 2017-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8831815
关键词：
Adverse effects Alleles Analysis of Variance Animal Model Biological Biological Assay Biological Models Chemical Exposure Chromosome Mapping Complex DNA Methylation Data Diethylstilbestrol Endocrine Disruptors Environment Environmental Exposure Epigenetic Process Equilibrium Estrogens Exposure to Gene Expression Gene Expression Regulation Genes Genetic Genetic Models Genetic Variation Genome Goals Health Human Inbred Mouse Inbred Strain Inbred Strains Mice Inbreeding Individual Knowledge Link Male Genital Organs Maps Measures Mentors Methods Modeling Molecular Molecular Biology Molecular Weight Mus Neonatal Pathway interactions Phenotype Physiological Physiology Play Population Population Control Population Heterogeneity Postdoctoral Fellow Predisposition Property Protocols documentation Quantitative Trait Loci Randomized Reproducibility Reproduction Reproductive Physiology Reproductive system Research Resources Role Series Sperm Count Procedure System Testing Testis Tissues Translating Variant bisulfite consumer product gene environment interaction insight mRNA Expression male reproductive response trait xenoestrogen

项目摘要

Title: Epigenetics, environmental exposure, and reproduction in the Collaborative Cross PI/PD: David L. Aylor The proposed research is a systems genetics approach to characterize the complex relationships between genetic variation, epigenetic regulation, gene expression, male reproductive physiology, and environmental exposure. To accomplish this goal, we will leverage the power of diverse inbred mouse strains and the Collaborative Cross, a mouse reference population that has ideal properties for systems genetics. To identify gene-by-environment (G¿E) interactions, we will perturb the reproductive system with the powerful estrogen diethylstilbestrol (DES) and phenotype identical exposed and control populations. We will measure DNA methylation, mRNA expression, and reproductive traits with the purpose of modeling genetic effects and G¿E across three levels of genome function. This research will produce new biological knowledge of the genetic networks underlying the reproductive system, DES function at the molecular level, and genes underlying DES sensitivity.

标题：协作中的表观遗传学，环境暴露和繁殖叉 PI/PD：David L. Aylor 拟议的研究是一种系统遗传学方法来表征复杂遗传变异，表观遗传调节，基因表达，男性之间的关系生殖生理和环境暴露。为了实现这一目标，我们将利用潜水员近交小鼠菌株和协作十字架的力量小鼠参考种群具有系统遗传学的理想特性。识别基因环境（g。）相互作用，我们将与复制系统扰动功能强大的雌激素二乙基贝贝氏（DES）和表型相同的暴露和控制人群。我们将测量DNA甲基化，mRNA表达和生殖特征的目的是建模遗传效应，并在三个基因组功能水平。这项研究将产生有关的新生物学知识复制系统的基因网络，DES在分子上的功能水平和基因敏感性。