A New Model for Elucidating the Genetic Mechanisms of Hybrid Male Sterility in House Mice

阐明家鼠杂交雄性不育遗传机制的新模型

基本信息

批准号：
10709906
负责人：
David Lawrence Aylor
金额：
$ 19万
依托单位：
NORTH CAROLINA STATE UNIVERSITY RALEIGH
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-23 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10709906
关键词：
Affect Alleles Animals Biological Breeding Cells Chromosomes Compensation Data European Exhibits Female Fertility Future Generations Genes Genetic Genetic Polymorphism Genetic Recombination Genetic Variation Genetic study Genomics Genotype Germ Cells Goals House mice Human Human Biology Hybrids Impairment Inbred Strain Inbred Strains Mice Infertility Male Infertility Male Sterility Male Sterilizations Maps Meiosis Microtus Modeling Molecular Mouse Strains Mus Nature Partner in relationship Phenotype Play Population Process Quantitative Trait Loci Reproductive Biology Research Research Project Grants Resolution Resources Role Spermatogenesis Spermatogenic Cell Sterility Synaptonemal Complex Variant cell type design experimental study genetic architecture genetic variant male novel offspring prevent public health relevance reproductive segregation trait transcriptome sequencing

项目摘要

PROJECT SUMMARY A major goal in evolutionary genetics is to identify the genetic variants that give rise to hybrid sterility, and to understand their mechanisms of action. Hybrid sterility is important because it maintains barriers between species in nature. The house mouse, Mus musculus, presents a unique opportunity to study the genetics of hybrid sterility due to the substantial but incomplete reproductive isolation between two subspecies (M.m.musculus and M.m.domesticus). Male offspring born to a musculus female and a domesticus male often exhibit hybrid male sterility (HMS). This phenomenon can be replicated experimentally using inbred mouse strains that are representative of each subspecies. Two major genetic factors have been shown to cause HMS, but many of the genes involved remain unknown. We recently identified a specific type of hybrid male that has the known HMS-associated QTL genotypes, yet is fertile and shows no reproductive impairment. The goals of this exploratory research project are to identify the genetic variants that rescue fertility in this hybrid, to identify the stage of spermatogenesis affected by those variants, and to identify the molecular differences between infertile and fertile hybrid males. These results will advance our understanding of evolutionary genetics, reproductive biology, and meiosis.

项目摘要进化遗传学的主要目标是确定引起杂种不育的遗传变异体，并了解他们的行动机制。混合不育很重要，因为它保持障碍在自然界中的物种之间。鼠标穆斯·穆斯库鲁斯（Mus Musculus）为学习提供了独特的机会由于两个之间的实质但不完全的生殖隔离而引起的混合无菌遗传学亚种（M.M.Musculus和M.M.Domistus）。男性的后代是肌肉女性和一个家乡男性经常表现出混合雄性不育（HMS）。这种现象可以复制实验使用代表每个亚种的近交小鼠菌株。已经证明两个主要的遗传因素会引起HM，但许多涉及的基因仍然存在未知。我们最近确定了具有已知HMS相关QTL的特定类型的杂化雄性基因型却是肥沃的，并且没有表现出生殖损害。这项探索性研究的目标项目旨在确定在这种杂种中挽救生育能力的遗传变异，以确定由这些变体影响的精子发生，并确定不育之间的分子差异和肥沃的杂种男性。这些结果将促进我们对进化遗传学的理解，生殖生物学和减数分裂。