Multi-functional particles for stem cell isolation and expansion

用于干细胞分离和扩增的多功能颗粒

• 批准号: 8644605
• 负责人: Wenjing Hu
• 金额: $ 20.03万
• 依托单位: PROGENITEC, INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-11 至 2017-07-10
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8644605
• 关键词: 3-Dimensional; Allylamine; Antibodies; Applied Research; Architecture; Atherosclerosis; Basic Science; Binding; Blood; Blood Vessel Prosthesis; CD34 gene; Cardiovascular Diseases; Cell Culture Techniques; Cell Separation; Cell Therapy; Cell Transplantation; Cell-Matrix Junction; Cells; Centrifugation; Chemicals; Development; Disease; Effectiveness; Enzymes; Evaluation; Goals; Human; In Vitro; Knowledge; Magnetism; Mechanics; Medical; Medical Research; Methods; Modeling; Myocardial Infarction; Outcome; Phase; Polymers; Polystyrenes; Preclinical Drug Evaluation; Process; Property; Research; Small Business Technology Transfer Research; Source; Stem Cell Research; Stem cells; Structure; Surface; System; Temperature; Therapeutic; Tissues; Work; bone; cell type; cellular engineering; design; improved; innovation; magnetic cell separation; nanoparticle; novel; particle; peripheral blood; phase 1 study; poly-N-isopropylacrylamide; public health relevance; tool

DESCRIPTION (provided by applicant): The objective of this STTR Phase I proposal is to develop novel multi-layer microparticles and demonstrate their feasibility to isolate and expand stem cells for medical research and therapeutic applications. In recent years, stem cells have been used in a wide variety of applications, including cell- based therapies, drug screening, and models to understand the disease development. Unfortunately, many of these applications require large number of stem cells which cannot be produced by most of the current cell isolation and culture methods. The limitations of the current methods include the use of harmful chemicals and enzymes for cell isolation and expansion, and harsh shear forces from centrifugation during cell passaging process. To overcome these drawbacks, we will develop novel multi-layer microparticles that allow stem cell isolation via magnetic separation, cell attachment using a temperature-sensitive mechanism that binds cells at 370C, cell expansion via 3-D structural architecture, and cell detachment by a temperature-sensitive mechanism to release cells bound to the surface at room temperature. These microparticles will provide "all-in-one pot" for cell isolation, expansion, and detachment of stem cells for use in medical applications without the use of harmful chemicals and mechanical forces. For the phase I study, we will formulate and characterize these multilayer microparticles (Aim 1). The microparticles will then be evaluated for their effectiveness in isolation, expansion, and detachment of stem cells, including endothelial progenitor cells (EPCs) from human blood (Aim 2). The successful completion of this proposal may provide a means to effectively isolate and expand various cell types in addition to stem cells. The knowledge gained from this research should significantly improve our understanding of magnetic cell separation, stem cell expansion, and microparticles for cellular engineering applications.

描述（由申请人提供）：该STTR I期提案的目的是开发新型的多层微粒，并证明其可行性可以隔离和扩展干细胞以进行医学研究和治疗应用。近年来，干细胞已用于多种应用，包括基于细胞的疗法，药物筛查和模型以了解疾病的发展。不幸的是，其中许多应用都需要大量的干细胞，而这些干细胞无法由当前的细胞分离和培养方法产生。当前方法的局限性包括使用有害化学物质和酶进行细胞分离和膨胀，以及在细胞传代过程中离心的刺激性剪切力。为了克服这些缺点，我们将开发新型的多层微粒，这些微粒可以通过磁分离，使用温度敏感的机制通过磁分离，细胞附着，该机制在370C下结合细胞，通过3-D结构结构扩展细胞，并通过A通过A脱离。温度敏感的机制，以释放在室温下结合到表面的细胞。这些微粒将提供“多合一的锅”，用于在不使用有害化学物质和机械力的情况下用于医疗应用中使用干细胞的细胞分离，扩张和脱离。对于I期研究，我们将制定和表征这些多层微粒（AIM 1）。然后，将评估微粒的干细胞分离，扩张和脱离的有效性，包括人类血液中的内皮祖细胞（EPC）（AIM 2）。该提案的成功完成可能提供了一种手段，除了干细胞外，除了干细胞外，还可以有效地隔离和扩展各种细胞类型。从这项研究中获得的知识应显着提高我们对磁细胞分离，干细胞扩展和用于细胞工程应用的微粒的理解。