Controlling Naturally-Derived Polymer Enzymatic Degradation: A Plasma-Enhanced Chemical Vapor Deposition Approach

控制天然聚合物酶降解：等离子体增强化学气相沉积方法

• 批准号: 10654781
• 负责人: Morgan J Hawker
• 金额: $ 14万
• 依托单位: CALIFORNIA STATE UNIVERSITY FRESNO
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10654781
• 关键词: Address; Adsorption; Alcohols; Allylamine; American; Area; Award; Behavior; Biochemical; Biocompatible Materials; Biological; Biological Models; Biopolymers; Body Regions; Cellulose; Chemicals; Chemistry; Chitin; Collaborations; Collagen; Data; Deposition; Development; Device Removal; Devices; Dryness; Environment; Enzyme Interaction; Enzymes; Excision; Exhibits; Exposure to; Fibroins; Film; Funding; Future; Gases; Goals; Health; Human; Hydrolysis; Hydrophobic Surfaces; Hydrophobicity; Implant; In Vitro; Infection; Life; Liquid substance; Measures; Mechanics; Mediating; Medical Device; Mentors; Mentorship; Methodology; Methods; Modeling; Modification; Morphology; Organ; Pain; Pentanes; Pharmaceutical Preparations; Plasma; Plasma Enhancement; Play; Polymer Chemistry; Polymers; Positioning Attribute; Postdoctoral Fellow; Predisposition; Process; Pronase; Property; Proteins; Public Health; Reporting; Research; Research Personnel; Resources; Roentgen Rays; Role; Route; Scanning Electron Microscopy; Silk; Solid; Spectroscopy, Fourier Transform Infrared; Spectrum Analysis; Structure; Surface; Surface Properties; System; Techniques; Testing; Thinness; Time; Tissues; Tooth Extraction; United States National Institutes of Health; Wettability; Work; biodegradable polymer; career; chymotrypsin A; crosslink; experience; flexibility; functional group; graduate student; healthcare-associated infections; hip replacement arthroplasty; hydrophilicity; implant material; implantable device; improved; innovation; insight; ionization; mechanical properties; medical implant; pressure; programs; repaired; response; side effect; success; undergraduate student; vapor; virtual; wound healing

Project Summary Biomaterial implants are natural or synthetic materials that can be placed in the body to improve human health in multiple ways, including delivering drugs to targeted regions of the body, healing wounds, and maintaining organ function. It is important that implants do not break down until they have performed their function. For example, stitches after tooth extractions can dissolve in a few weeks, but a hip replacement implant should be able to stay intact for years. When these implants degrade too quickly or not quickly enough, complications such as pain and infection can occur. The goal of our research is to address the need to develop implants for different applications by making biomaterials with a range of degradation times. The materials proposed in this research are made of silk, a naturally-derived material that interacts with the body without negative side effects. When silk is placed in the body, it is degraded by enzymes. The goal of our research is to create a range of silk materials with different degradation rates. Our approach is to control how enzymes access the silk surface by changing the atoms and molecules in the silk film surface. The method that will be used to change the chemistry of the silk surface is plasma-enhanced chemical vapor deposition (PECVD), which is a technique used to apply a thin coating to the silk with specific types of atoms. Silk film surface chemistry, wettability, and morphology will be assessed before and after PECVD to characterize any changes in the material. Following PECVD, silk films and untreated control films will be weighed and exposed to enzyme-containing solutions. Films will be removed from solution, dried, and weighed again to measure how much of the material has degraded. The specific objective of the proposed work is to use PECVD to customize the silk surface chemistry, thus controlling how the enzymes interact with the silk materials. The central hypothesis for this proposal is twofold 1) introducing a hydrophobic coating to silk films will decrease the rate of enzymatic degradation, and 2) introducing a hydrophilic coating to silk films will increase the rate of enzymatic degradation. This hypothesis is based on the ability for enzymes to adsorb to the silk film surface, which ultimately controls the enzymatic degradation of the film. This proposal is expected to result in a PECVD method to customize the degradation rate of silk through controlling the film chemistry. Our strategy is expected to inform a range of implant applications. This work will help to address our long-term research goal: to understand how tuning naturally-derived material (e.g., collagen, chitin, cellulose) surface chemistry controls susceptibility to enzymatic degradation. The proposed research will position me to be competitive for future awards (e.g., SC1, R15, R01) so that I can pursue this long-term goal. As I am in the beginning stage of my career, this proposal will provide me with the resources to establish myself as an independent researcher so that I can provide high-quality research experience to undergraduate and graduate students for many years to come.

项目摘要 生物材料植入物是天然或合成材料，可以放置在体内以改善人类健康 以多种方式，包括向人体的靶向区域输送药物，治愈伤口和维护 器官功能。重要的是，植入物在执行功能之前不会分解。为了 例如，拔牙后的针迹可以在几周内溶解，但是髋关节置换植入物应为 能够保持完整多年。当这些植入物过快或不够快速降解时，并发症就是这样 可能会发生疼痛和感染。我们研究的目的是满足开发不同的植入物的需求 通过制造具有一系列降解时间的生物材料来应用。这项研究提出的材料 是由丝绸制成的，丝绸是一种自然衍生的材料，与身体相互作用而没有负面影响。当丝绸时 被放置在体内，被酶降解。我们研究的目的是创建一系列丝绸材料 有不同的降解率。我们的方法是控制酶如何通过改变丝表面 丝膜表面的原子和分子。将用于改变化学的方法 丝表面是血浆增强化学蒸气沉积（PECVD），这是一种用于施用薄的技术 用特定类型的原子涂上丝绸。丝绸膜表面化学，润湿性和形态将是 在PECVD之前和之后进行评估，以表征材料中的任何变化。遵循PECVD，丝绸电影和 未处理的对照膜将被称重并暴露于含酶的溶液中。电影将从 溶液，干燥并再次称重，以测量多少材料降解。具体目标 拟议的工作是使用PECVD自定义丝表面化学，从而控制酶如何 与丝绸材料相互作用。该提议的中心假设是双重的1） 疏水涂层到丝绸膜将降低酶促降解的速率，2）引入A 亲水性涂层到丝绸膜将增加酶促降解的速率。该假设是基于 关于酶吸附到丝绸膜表面的能力，该表面最终控制酶促降解 电影。预计该建议将导致一种PECVD方法来自定义丝绸的降解率 通过控制膜化学。我们的策略有望为一系列植入应用提供信息。 这项工作将有助于解决我们的长期研究目标：了解如何调整自然衍生的材料 （例如，胶原蛋白，几丁质，纤维素）表面化学控制着酶促降解的敏感性。这 拟议的研究将使我将未来奖项具有竞争力（例如SC1，R15，R01），以便我可以追求 这个长期目标。当我处于职业生涯的开始时，该建议将为我提供资源 确立自己为独立研究人员，以便我可以提供高质量的研究经验 未来很多年的本科生和研究生。

项目成果

Using 1,8-cineole plasma with both pulsed and continuous depositions to modify commercially available wound dressing materials.

使用 1,8-桉树脑等离子体进行脉冲和连续沉积来改性市售伤口敷料材料。

• DOI: 10.1116/6.0003009
• 发表时间: 2023
• 期刊: Biointerphases
• 影响因子: 2.1
• 作者: Kayaian,Mia-Rose;Hawker,MorganJ
• 通讯作者: Hawker,MorganJ

Evaluating hydrophobic recovery of N2 and H2O(g) plasma modified silk fibroin films aged at ambient and elevated temperatures.

评估在环境温度和高温下老化的 N2 和 H2O(g) 等离子体改性丝素蛋白膜的疏水性恢复。

• DOI: 10.1116/6.0002803
• 发表时间: 2023
• 期刊: Journal of vacuum science & technology. A, Vacuum, surfaces, and films : an official journal of the American Vacuum Society
• 作者: Keobounnam,AshleyN;Lenert-Mondou,Chase;Kubik,Alexzandria;Hawker,MorganJ
• 通讯作者: Hawker,MorganJ