The Exercise and Physical Activity Collaborative Team (ExPACT): a Proposed MoTrPAC Clinical Center

锻炼和身体活动协作团队 (ExPACT)：拟建 MoTrPAC 临床中心

• 批准号: 10893749
• 负责人: Thomas W Buford
• 金额: $ 9.78万
• 依托单位: BALL STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2023-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10893749
• 关键词: Acute; Aerobic; Alabama; Ancillary Study; Biological; Clinical; Clinical Trials; Collaborations; Collection; Coupled; Data; Deposition; Diabetes Mellitus; Disease; Enrollment; Ethnic Origin; Exercise; Florida; Future; Geography; Goals; Health; Health Benefit; Human; Information Dissemination; Knowledge; Laboratories; Leadership; Link; Manuscripts; Maps; Medicine; Metabolism; Methodology; Molecular; Outcome; Performance; Phase; Phenotype; Physical activity; Population; Procedures; Public Health; Quality Control; Race; Research Institute; Resistance; Rural; Seasons; Structure; Testing; Tissues; Training; Transducers; Translational Research; United States National Institutes of Health; Universities; Woman; Work; Writing; circadian; clinical center; clinical training; design; exercise training; experience; fitness; human tissue; improved; innovation; men; preservation; randomized trial; recruit; resistance exercise; response; sedentary; suburb

Project Summary The Molecular Transducers of Physical Activity Consortium (MoTrPAC) is designed to discover and characterize the range of molecular transducers (the ‘molecular map’) that underlie the effects of physical activity (PA) in humans. Our Exercise and Physical Activity Collaborative Team (ExPACT) –an alliance of the University of Alabama at Birmingham Center for Exercise Medicine (UAB), Translational Research Institute for Metabolism and Diabetes in Orlando, Florida (TRI-MD), and Ball State University Human Performance Laboratory (BSU) –has extensive experience performing highly-controlled aerobic (AT) and resistance (RT) exercise training clinical trials in healthy and diseased populations. The overarching goal of ExPACT is to contribute substantively to successful MoTrPAC planning, execution, and innovation. Phase 1 has already been completed and consisted of: Aim 1) Effectively collaborate with MoTrPAC leaders and NIH leadership to help guide and finalize the structure and standard operating procedures (SOPs). In the remaining years of the project, the ExPACT will implement and complete Phase II and Phase III, as follows: Phase II (Recruitment and Testing Years) Aim 1. We will enroll 420 sedentary but healthy men and women 18+ y into a rigorously controlled, 12-wk, randomized trial of AT (n=140), RT (n=140), or no-exercise control (CON, n=50). ExPACT geography will enrich MoTrPAC racial, ethnic, urban, suburban, and rural diversity. The design incorporates comprehensive phenotyping to assess fitness and clinical outcomes, and human tissue phenotyping to link the molecular transducers with key biological responses to exercise. Aim 2. Pre-and posttraining, we will obtain strategically timed biospecimens before and after acute exercise. To better understand the biological extremes, we will conduct the same acute response studies in highly-trained athletes (AT (n=25) or RT (n=25)). Serial biospecimen collection in CON will allow estimation of biological, seasonal, circadian and methodological variability. Aim 3. To enhance the overall scientific impact of MoTrPAC, we will continue to work with the consortium to conduct ancillary studies, which will markedly augment subject and tissue phenotyping and interpretation of the molecular maps. Phase III (Close-out) Aim 1. We will complete all ExPACT data entry, quality control, and central deposit procedures in collaboration with the MoTrPAC Coordinating Center, and we will actively contribute to the completion of biospecimen analyses, manuscript writing, and dissemination of results. We would be honored to collaborate with MoTrPAC to contribute future knowledge on the molecular mechanisms through which physical activity can improve or preserve health.

项目概要 身体活动分子传感器联盟 (MoTrPAC) 旨在发现 并描述了构成其基础的分子传感器的范围（“分子图”） 体力活动 (PA) 对人类的影响。我们的锻炼和体力活动协作。 团队 (ExPACT) – 阿拉巴马大学伯明翰运动中心联盟 医学（UAB），奥兰多代谢和糖尿病转化研究所， 佛罗里达州 (TRI-MD) 和鲍尔州立大学人类表现实验室 (BSU) – 拥有 具有进行高度控制的有氧 (AT) 和阻力 (RT) 运动的丰富经验 在健康和患病人群中进行临床试验培训 ExPACT 的总体目标。 的目标是为 MoTrPAC 的成功规划、执行和创新做出实质性贡献。 第一阶段已经完成并开始： 目标 1) 与 MoTrPAC 领导人和 NIH 领导层帮助指导和最终确定结构和标准 在项目的剩余年份中，ExPACT 将实施操作程序 (SOP)。 并完成第二阶段和第三阶段，如下： 第二阶段（招募和测试年）目标 1. 我们将把 420 名 18 岁以上久坐但健康的男性和女性纳入一个严格控制的、 12 周，AT（n=140）、RT（n=140）或无运动对照（CON，n=50）的随机试验。 ExPACT 地理将丰富 MoTrPAC 种族、民族、城市、郊区和农村的多样性。 该设计结合了全面的表型分析来评估健康和临床结果， 和人体组织表型分析，将分子传感器与关键生物反应联系起来 目标2.训练前和训练后，我们将有策略地在训练前获取定时生物样本。 在剧烈运动后，为了更好地了解生物学极端情况，我们将进行 在训练有素的运动员中进行了相同的急性反应研究（AT（n = 25）或RT（n = 25）系列）。 CON 中的生物样本收集将能够估计生物、季节、昼夜节律和 目标 3. 为了增强 MoTrPAC 的整体科学影响，我们将 继续与该联盟合作进行辅助研究，这将显着 增强的受试者和组织表型分析以及分子图谱的解释第三阶段。 （结束）目标 1. 我们将完成所有 ExPACT 数据录入、质量控制和集中存款 与 MoTrPAC 协调中心合作制定程序，我们将积极 有助于完成生物样本分析、手稿撰写和传播 我们很荣幸与 MoTrPAC 合作，贡献未来的知识。 身体活动可以改善或保持健康的分子机制。

项目成果

Isolation of nuclei from frozen human subcutaneous adipose tissue for full-length single-nuclei transcriptional profiling.

从冷冻人皮下脂肪组织中分离细胞核，进行全长单核转录分析。

• 发表时间: 2023-03-17
• 作者: Whytock, Katie L;Divoux, Adeline;Sun, Yifei;Hopf, Meghan;Yeo, Reichelle X;Pino, Maria F;Yu, GongXin;Smith, Steven R;Walsh, Martin J;Sparks, Lauren M
• 通讯作者: Sparks, Lauren M

Influence of low-dose aspirin, resistance exercise, and sex on human skeletal muscle PGE2 /COX pathway activity.

小剂量阿司匹林、抗阻运动和性对人体骨骼肌 PGE2/COX 通路活性的影响。

• 发表时间: 2021-03
• 影响因子: 2.5
• 作者: Naruse, Masatoshi;Fountain, William A;Claiborne, Ale;Chambers, Toby L;Jones, Andrew M;Stroh, Andrew M;Montenegro, Cristhian F;Lynch, Colleen E;Minchev, Kiril;Trappe, Scott;Trappe, Todd A
• 通讯作者: Trappe, Todd A

Pyruvate Kinase M2 Supports Muscle Progenitor Cell Proliferation but Is Dispensable for Skeletal Muscle Regeneration after Injury.

丙酮酸激酶 M2 支持肌肉祖细胞增殖，但对于受伤后的骨骼肌再生来说是可有可无的。

• DOI: 10.1093/jn/nxab251
• 发表时间: 2021-08-12
• 期刊: The Journal of nutrition
• 作者: Jamie E Blum;Br;on J. Gheller;on;Abby Benvie;M. Field;E. Panizza;Nathaniel M. Vacanti;Daniel Berry;A. Thalacker
• 通讯作者: A. Thalacker

Muscle transcriptional networks linked to resistance exercise training hypertrophic response heterogeneity.

肌肉转录网络与阻力运动训练肥大反应异质性相关。

• 发表时间: 2021
• 影响因子: 4.6
• 作者: Lavin, Kaleen M;Bell, Margaret B;McAdam, Jeremy S;Peck, Bailey D;Walton, R Grace;Windham, Samuel T;Tuggle, S Craig;Long, Douglas E;Kern, Philip A;Peterson, Charlotte A;Bamman, Marcas M
• 通讯作者: Bamman, Marcas M

Human adipose and skeletal muscle tissue DNA, RNA, and protein content.

人体脂肪和骨骼肌组织 DNA、RNA 和蛋白质含量。

• 发表时间: 2021
• 作者: Stroh, Andrew M;Lynch, Colleen E;Lester, Bridget E;Minchev, Kiril;Chambers, Toby L;Montenegro, Cristhian F;Chavez Martinez, Clarisa;Fountain, William A;Trappe, Todd A;Trappe, Scott W
• 通讯作者: Trappe, Scott W