Development of 3D electronic atlases of deveoping mouse brains

开发小鼠大脑发育的 3D 电子图谱

基本信息

批准号：
8248494
负责人：
hangyi jiang
金额：
$ 14.1万
依托单位：
ANATOMYWORKS, LLC
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-10 至 2014-03-09
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8248494
关键词：
Adult Agreement Anatomy Animals Atlases Brain Brain Pathology Cells Chemicals Communities Computer software Consult Copyright Data Databases Development Devices Electrodes Electronics Embryo Engineered Gene Ensure Feedback Goals Head Histology Image Individual Investigation Label Licensing Location Magnetic Resonance Imaging Manuals Marketing Measurement Measures Morus Mus Needles Neuraxis Neurosciences Phase Population Positioning Attribute Primates Process Reading Research Research Design Resources Role Schedule Slice Source Staging Structure System Technology Testing Transplantation Work Writing base blind cranium design in vivo interest mouse model mutant neonate operation phase 2 study product development programs sample fixation software development stem two-dimensional vector virtual

Adult Agreement Anatomy Animals Atlases Brain Brain Pathology Cells Chemicals Communities Computer software Consult Copyright Data Databases Development Devices Electrodes Electronics Embryo Engineered Gene Ensure Feedback Goals Head Histology Image Individual Investigation Label Licensing Location Magnetic Resonance Imaging Manuals Marketing Measurement Measures Morus Mus Needles Neuraxis Neurosciences Phase Population Positioning Attribute Primates Process Reading Research Research Design Resources Role Schedule Slice Source Staging Structure System Technology Testing Transplantation Work Writing base blind cranium design in vivo interest mouse model mutant neonate operation phase 2 study product development programs sample fixation software development stem two-dimensional vector virtual

展开

项目摘要

DESCRIPTION (provided by applicant): The overall goal of this application is to develop electronic mouse atlases for image-guided stereotaxic operations. In mouse-based neuroscience studies, stereotaxic operations are an essential technology, in which chemicals, vectors, cells, or electrodes are delivered to specific brain structures of interest. Because the delivery is essentially a blind operation, stereotaxic atlases are an essential resource of the studies. Currently, Paxinos atlas is almost exclusively used for this purpose. However, this atlas has several limitations. First of all, it covers only adult brains and at present there is no stereotaxic atlas available for neonate brains. Second, it is a histology-based two-dimensional atlas. It is almost impossible to design an oblique needle path to avoid damaging important brain structures based on the 2D information. Third, the fixation and sectioning process introduced inaccuracy in the brain coordinates. Forth, the atlas is based on one animal for each sectioning orientation. Therefore, anatomical variability among individual mouse brains is not considered. In this project, we will introduce fully three-dimensional electronic atlases of mouse brains at different developmental stages for image-guided stereotaxic operations. Because the coordinates of stereotaxic operations are based on anatomical landmarks on the skull, co-registered CT (for skull structures) and MRI (for brain structures) data ensure the accurate coordinate systems. The brain anatomy is based on in vivo MRI of multiple mice, providing the population-representative anatomy of the undistorted (not fixed and not sectioned) brain anatomy. Our software, AtlasGuide, provides various advanced functions such as "virtual needle path" for pre-operation planning and oblique-angle needle guidance. Currently, we are in the process of product development agreement with Stoelting. In this phase I application, we will perform following three aims. Aim 1: To write a manual and introduce the first product While the first product is already completed and rigorously tested, the manual making of this product with a wide array of features requires a considerable amount of effort. Currently, the manual is the only missing piece for the product introduction. In this aim, a comprehensive manual will be written and we will work with Stoelting for the product introduction. Aim 2: To develop user-interface for advanced image-guided operations In this Aim, we will implement a new feature, landmark-based positioning system, which will be a key function for Aim 3 and the Phase II study. Aim 3: To implement a function to read user-defined MR images This new function will allow users to load their own MRI data. This will be especially important for users using mouse models with altered brain anatomy (e.g. mutants) or brains of other species such as primates. PUBLIC HEALTH RELEVANCE: We will develop software called AtlasGuide. This enables accurate needle or electrode insertion to mouse brains at different developmental stages. This type of studies is important to investigate how brain works and understand mechanisms of brain pathology.

描述（由申请人提供）：此应用程序的总体目标是开发用于图像引导的立体定位操作的电子鼠标图书馆。在基于小鼠的神经科学研究中，立体定位操作是一项必不可少的技术，其中化学物质，载体，细胞或电极被输送到感兴趣的特定大脑结构中。由于交付本质上是盲目的操作，因此立体定量地图集是研究的重要资源。目前，Paxinos Atlas几乎仅用于此目的。但是，该地图集有几个限制。首先，它仅覆盖成人大脑，目前尚无立体定义地图集可用于新生儿大脑。其次，它是基于组织学的二维图集。设计斜针路几乎不可能避免根据2D信息损害重要的大脑结构。第三，固定和切片过程引入了大脑坐标的不准确性。第四，地图集是基于每种切片方向的一种动物。因此，不考虑各个小鼠大脑之间的解剖学变异性。在这个项目中，我们将介绍小鼠的全部三维电子图书馆在不同发育阶段进行图像引导的立体定位操作的大脑。因为立体静脉操作的坐标基于头骨上的解剖标志，因此共同注册的CT（用于头骨结构）和MRI（对于大脑结构）数据确保了准确的坐标系统。大脑解剖结构是基于多只小鼠的体内MRI，提供了人口代表性的解剖结构，该解剖结构是未经突出的（不是固定和未切除）脑解剖结构的。我们的软件AtlasGuide提供了各种高级功能，例如“虚拟针路”，用于操作前计划和倾斜角针指导。目前，我们正在签订产品开发协议，并进行了斯托特。在此I阶段应用程序中，我们将执行以下三个目标。 AIM 1：编写手册并介绍第一批产品，而第一个产品已经完成并经过严格测试，使用各种功能的该产品的手册制造需要大量的努力。目前，该手册是产品介绍的唯一缺少的作品。在此目标中，将制定一本全面的手册，我们将与产品介绍一起进行。目标2：为了开发用于此目标的高级图像指导操作的用户界面，我们将实施一个新功能，基于里程碑的定位系统，这将是AIM 3和II阶段研究的关键功能。 AIM 3：要实现一个函数来读取用户定义的MR图像，此新功能将允许用户加载自己的MRI数据。对于使用脑解剖结构改变的小鼠模型（例如突变体）或其他物种（例如灵长类动物）的大脑的用户，这将尤其重要。公共卫生相关性：我们将开发名为AtlasGuide的软件。这可以使针头或电极在不同发育阶段的小鼠大脑插入。这种类型的研究对于研究大脑的工作方式和理解脑病理的机制非常重要。