Comparative Biology of Tissue Repair, Regeneration and Aging

组织修复、再生和衰老的比较生物学

• 批准号: 10849947
• 负责人: IAIN A. DRUMMOND
• 金额: $ 90.03万
• 依托单位: MOUNT DESERT ISLAND BIOLOGICAL LAB
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10849947
• 关键词: Aging; Ambystoma; Award; Biological Metamorphosis; Biology; Centers of Research Excellence; Comparative Biology; Comparative Study; Computational Biology; Data; Data Set; Faculty; Fibrosis; Functional disorder; Future; Genomics; Goals; Grant; Injury; Knowledge; Maps; Methodology; Modeling; Molecular; Mus; Natural regeneration; Parents; Pathway interactions; Regenerative capacity; Role; Scientist; Skin; Skin Tissue; Stress; System; Technology; Thyroid Gland; Thyroid Hormones; Tissues; Update; Work; comparative; experimental study; functional genomics; hormonal signals; insight; model organism; mouse model; multiple omics; next generation; tissue repair; transcriptome; wound healing

Project Summary The goal of the COBRE-II parent award at MDIBL is to study the intersection of stress, aging, and regeneration using comparative functional genomic studies of appropriate model organism systems. In the current proposal, Dr. Murawala (RPL within COBRE II at MDIBL) will team up with Dr. Arturo Hernandez (Faculty scientist III, MHIR) and Dr. Joel Graber (Director, MDIBL Computational Biology Core) to perform comparative studies the effect of thyroid hormone-induced metamorphosis in axolotl and mouse using next-generation methodologies. Dr. Murawala will focus on understanding the thyroid-hormone induced changes in axolotl skin using multiome and spatial transcriptome technology of 10X Genomics, Dr. Hernandez will perform complementary experiments in mouse models, and Dr. Graber will refine the axolotl-mouse orthology mapping and will use this updated information to analyze and compare multiome and spatial data from the Murawala lab and Hernandez labs. The work proposed here will allow us to generate valuable comparative datasets that will 1) provide crucial insights into pathophysiology of skin upon abnormal thyroid hormone signaling and 2) form the basis for future larger grants aimed at elucidating the role and underlying mechanisms by which thyroid hormones regulate tissue maturation and regenerative capacity.

项目概要 MDIBL 设立 COBRE-II 家长奖的目标是研究压力、衰老和 使用适当模型生物系统的比较功能基因组研究进行再生。在 目前的提案中，Murawala 博士（MDIBL 的 COBRE II 内的 RPL）将与 Arturo Hernandez 博士（教员）合作 科学家 III，MHIR）和 Joel Graber 博士（MDIBL 计算生物学核心主任）进行比较 使用下一代研究甲状腺激素诱导的蝾螈和小鼠变态的影响 方法论。 Murawala 博士将重点研究甲状腺激素引起的蝾螈皮肤变化 Hernandez 博士将利用 10X Genomics 的多组和空间转录组技术进行 小鼠模型中的补充实验，Graber 博士将完善蝾螈-小鼠直系图谱 并将使用这些更新的信息来分析和比较来自 Murawala 实验室的多组和空间数据 和埃尔南德斯实验室。 这里提出的工作将使我们能够生成有价值的比较数据集，这些数据集将 1) 提供 对异常甲状腺激素信号传导引起的皮肤病理生理学的重要见解，2）构成了基础 未来更多的赠款旨在阐明甲状腺激素调节的作用和潜在机制 组织成熟和再生能力。

项目成果

Wound-Induced Polyploidization: Regulation by Hippo and JNK Signaling and Conservation in Mammals.

伤口诱导的多倍化：哺乳动物中河马和 JNK 信号传导和保护的调节。

• 发表时间: 2016
• 影响因子: 3.7
• 作者: Losick, Vicki P;Jun, Albert S;Spradling, Allan C
• 通讯作者: Spradling, Allan C

On the meaning of chance in biology.

论生物学中机会的含义。

• 发表时间: 2014-12-01
• 影响因子: 1.6
• 作者: Coffman; James A
• 通讯作者: James A

Pharmacological targeting of actin-dependent dynamin oligomerization ameliorates chronic kidney disease in diverse animal models.

肌动蛋白依赖性动力寡聚化的药理学靶向可改善多种动物模型中的慢性肾病。

• 发表时间: 2015-06
• 影响因子: 82.9
• 作者: Schiffer, Mario;Teng, Beina;Gu, Changkyu;Shchedrina, Valentina A;Kasaikina, Marina;Pham, Vincent A;Hanke, Nils;Rong, Song;Gueler, Faikah;Schroder, Patricia;Tossidou, Irini;Park, Joon;Staggs, Lynne;Haller, Hermann;Erschow, Sergej;Hilfik
• 通讯作者: Hilfik

Transient laminin beta 1a Induction Defines the Wound Epidermis during Zebrafish Fin Regeneration.

瞬时层粘连蛋白 beta 1a 诱导定义了斑马鱼鳍再生过程中的伤口表皮。

• 发表时间: 2015-08
• 影响因子: 4.5
• 作者: Chen, Chen;Merriman, Alexander F;Savage, Jeremiah;Willer, Jason;Wahlig, Taylor;Katsanis, Nicholas;Yin, Viravuth P;Poss, Kenneth D
• 通讯作者: Poss, Kenneth D

Anabolic Function Downstream of TOR Controls Trade-offs Between Longevity and Reproduction at the Level of Specific Tissues in C. elegans.

TOR 下游的合成代谢功能控制线虫特定组织水平上的寿命和繁殖之间的权衡。

• 发表时间: 2021-09
• 作者: Howard, Amber C;Mir, Dilawar;Snow, Santina;Horrocks, Jordan;Sayed, Hussein;Ma, Zhengxin;Rogers, Aric N
• 通讯作者: Rogers, Aric N