Mommyomics and Babyomics â Effect of Gestational Diabetes on the Developing Infant Microbiome

Mommyomics 和 Babyomics — 妊娠期糖尿病对发育中的婴儿微生物组的影响

• 批准号: 10848257
• 负责人: Kazune Catherine Pax
• 金额: $ 5.35万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10848257
• 关键词: Adolescent obesity; Adult; Affect; American; Antibiotics; Anxiety; Attention deficit hyperactivity disorder; Bacteria; Behavioral; Bioinformatics; Biometry; Birth trauma; Cesarean section; Child; Child Health; Child Welfare; Chronic; Clinical Research; Cognitive; Complex; Correlation Studies; Cross-Sectional Studies; Data; Dental caries; Development; Diabetes Mellitus; Disease; Disease susceptibility; Emotional; Famines; Future; Genes; Genetic; Gestational Diabetes; Glucose Intolerance; Glycosylated hemoglobin A; Habits; Health; Health Promotion; Heritability; Human; Human Genome; Human Microbiome; Hyperglycemia; Hypoglycemia; Infant; Infant Development; Intergenerational transfer; Intestines; Longitudinal Studies; Maps; Maternal Health; Mental Depression; Mental Retardation; Metabolic; Metabolic Diseases; Metagenomics; Modeling; Mothers; Mouth Diseases; Mus; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Oral cavity; Oral health; Pattern; Periodontitis; Pre-Eclampsia; Predisposition; Pregnancy; Pregnant Women; Prevalence; Process; Prospective Studies; Research; Research Design; Research Personnel; Risk; Risk Assessment; Risk Factors; Route; Sampling; Smoking; Source; Stressful Event; System; Testing; Time; Vertical Transmission; Whole-Genome Shotgun Sequencing; Woman; Women' s Health; adverse outcome; bacterial community; career; comparative; dynamic system; experience; feeding; gut microbiome; health determinants; impaired glucose tolerance; insulin signaling; interest; longitudinal, prospective study; malignant mouth neoplasm; maternal microbiome; microbiome; microbiome research; next generation sequencing; obesity in children; offspring; oral microbiome; oral pathogen; pathogen; pediatric trauma; prebiotics; pregnant; response; training opportunity; transmission process; traumatized children

Project Summary Maternal health is emerging as a critical long-term determinant of a child’s health. Therefore, promoting the health of women, especially pregnant women, promotes the well-being of children. The prevalence of gestational diabetes mellitus (GDM) has been steadily increasing, with current estimates at 13%. GDM is associated with adverse outcomes for both the mother and child. For example, there is an increased risk of pre-eclampsia and cesarean section for the mother and hypoglycemia, mental retardation, and birth trauma for the child. GDM has also been associated with an increased risk of future adolescent obesity for the child. Additionally, it has been found that GDM alters the maternal microbiomes, although the extent to which it impacts each ecological niche remains contested. Therefore, it is important to study the impact of GDM on the maternal microbiome to fully understand the impact of GDM at the metagenomic level. Furthermore, because there is intergenerational transfer of microbiomes and the microbiome is an important health determinant, studying the impact of GDM on the acquisition and development of the infant microbiome is critical to understanding risk for oral diseases. In the present proposal, it is hypothesized that the establishment of a stable, personalized infant oral microbiome is significantly influenced by maternal GDM and that the effects of GDM outweigh simple vertical transmission of the microbiome due to shared genetics between mothers and infants. This will be achieved by combining two independent yet integrated study designs with whole genome shotgun sequencing and strain-resolved comparative metagenomics. Both cross sectional and longitudinal study designs will be used to determine whether GDM impacts the oral microbiome, to determine the impact of maternal GDM on the developing infant oral microbiome, and to determine the relative attributions of vertical transmission and GDM to the developing infant oral microbiome. This will reveal the complexity and function of the microbiome at the gene level, stability over time and in response to perturbations, and to what extent the oral microbiome is a heritable feature, similar to the human genome. These findings will have major implications for disease and health including possible determinants of disease susceptibility, risk assessment and feasibility of strategies for prebiotics.

项目概要 孕产妇健康正在成为儿童健康的关键长期决定因素。 妇女特别是孕妇的健康可以促进儿童的福祉。 糖尿病 (GDM) 一直在稳步增加，目前估计 13% 与 GDM 相关。 对母亲和孩子都会产生不良后果，例如，先兆子痫和子痫的风险增加。 母亲剖腹产，孩子出现低血糖、智力低下和产伤。 也与儿童未来青少年肥胖的风险增加有关。 发现 GDM 改变了母体微生物组，尽管它影响每个生态位的程度 因此，充分研究 GDM 对母体微生物组的影响仍然存在争议。 了解 GDM 在宏基因组水平上的影响 此外，因为存在代际关系。 微生物组的转移以及微生物组是重要的健康决定因素，研究 GDM 对健康的影响 婴儿微生物组的获得和发育对于了解口腔疾病的风险至关重要。 在本提案中，开创性地建立了稳定的、个性化的婴儿口腔微生物组 受母亲 GDM 的影响显着，并且 GDM 的影响超过简单的垂直传播 由于母亲和婴儿之间的共同遗传，微生物组的变化将通过结合两者来实现。 独立但综合的研究设计，包括全基因组鸟枪测序和菌株解析 比较宏基因组学将使用横截面和纵向研究设计来确定。 GDM 是否影响口腔微生物组，以确定母亲 GDM 对发育中婴儿的影响 口腔微生物组，并确定垂直传播和 GDM 对发育中的相对归因 婴儿口腔微生物组。 这将揭示微生物组在基因水平上的复杂性和功能，以及随时间和时间的稳定性。 对扰动的反应，以及口腔微生物组在多大程度上是一种遗传特征，类似于人类 这些发现将对疾病和健康产生重大影响，包括可能的决定因素。 疾病易感性、风险评估和益生元策略的可行性。