N6-methyladenosine (m6A) Interplays with RNA and DNA Damage to Regulate DNA Repair

N6-甲基腺苷 (m6A) 与 RNA 和 DNA 损伤相互作用以调节 DNA 修复

• 批准号: 10835455
• 负责人: Yuan Liu
• 金额: $ 2.43万
• 依托单位: FLORIDA INTERNATIONAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-24 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10835455
• 关键词: Biological Assay; DNA Damage; DNA Modification Process; DNA Polymerase beta; DNA Repair; Deposition; Equipment; Funding; Genes; Goals; Grant; Laboratories; Maps; Mediating; Modeling; National Institute of Environmental Health Sciences; OGG1 gene; RNA; Request for Applications; Research; Services; Time; Transcript; Validation; cancer cell; knock-down; knockout gene; repaired; success

Project Summary The equipment supplement application requests funds to purchase a Bio-Rad real-time PCR machine that is essential for the validation of OGG1 gene knockout and METTL3 gene knockdown and for mapping the profiles of m6A and RNA and DNA damage on the DNA polymerase β (Pol β) gene in cancer cells under the aims of the PI’s NIEHS active grant R03ES0350200. The strand break-mediated RNA and DNA modification profiling assays (SBRM and SBRM) used for mapping m6A and RNA and DNA damage on the Pol β gene are PCR-based and critical for the success of the proposed studies. The old real-time PCR machine in the PI’s laboratory was broken recently. It cannot be repaired because the old model and its repair service were discontinued by Bio-Rad Laboratories. A new real- time PCR machine will allow us to accomplish the goals of the proposed studies on understanding the effects of RNA and DNA damage on m6A deposition on the Pol β gene and how m6A profiling changes can alter DNA repair by modulating RNA damage on the Pol β transcript in cancer cells. Thus, there is an urgent need to purchase the new real-time PCR machine that is essential for us to accomplish the research goals of our project on time.

项目概要 设备补充申请要求资金购买 Bio-Rad 实时 PCR 机 这对于验证 OGG1 基因敲除和 METTL3 基因敲除以及 绘制 DNA 聚合酶 β (Pol β) 基因上 m6A 和 RNA 和 DNA 损伤的图谱 PI 的 NIEHS 主动资助 R03ES0350200 的目标下的癌细胞。 RNA 和 DNA 修饰分析测定（SBRM 和 SBRM）用于绘制 m6A 和 RNA 以及 Pol β 基因上的 DNA 损伤是基于 PCR 的，对于拟议研究的成功至关重要。 PI实验室的旧实时PCR机最近坏了，无法修复。 因为旧型号及其维修服务已被 Bio-Rad 实验室终止。 时间 PCR 机将使我们能够实现所提出的研究目标，即了解 RNA 和 DNA 损伤对 Pol β 基因上 m6A 沉积的影响以及 m6A 分析如何变化 可以通过调节癌细胞中 Pol β 转录物上的 RNA 损伤来改变 DNA 修复。 迫切需要购买新的实时 PCR 机器，这对我们完成任务至关重要 我们项目的研究目标按时完成。