Development and Production of Standardized Reference Diets for Zebrafish Research

斑马鱼研究标准化参考饲料的开发和生产

• 批准号: 10823702
• 负责人: Donald Corace
• 金额: $ 129.2万
• 依托单位: MERIDIAN BIOTECH, LLC
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-15 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10823702
• 关键词: Academia; Address; Adolescent; Adoption; Adult; Affect; Alabama; Amino Acids; Animal Model; Applied Research; Aquaculture; Biomass; Biomedical Research; Biotechnology; Businesses; Canada; Caseins; Collaborations; Coupled; Data; Development; Diet; Dietary Proteins; Disease; Dryness; Farm; Fatty Acids; Fatty acid glycerol esters; Fishes; Formulation; Gel; Genetic; Goals; Growth; Health; Human; Immunocompetence; Industry; Intake; Investments; Laboratories; Marketing; Meridians; Modeling; Modernization; Mus; Nutrient; Nutritional; Organism; Outcome; Penetration; Phase; Phenotype; Physiological Processes; Production; Progress Reports; Proteins; Reference Standards; Reproducibility; Research; Science; Shrimp; Small Business Innovation Research Grant; Source; Standardization; Technology; Testing; Toxicology; United States National Institutes of Health; Universities; Vertebrates; Work; Zebrafish; cohort; commercialization; feeding; fortification; improved; lipid metabolism; manufacturing capabilities; manufacturing scale-up; microbial; microbiome; offspring; pharmacologic; physical property; programs; reproductive; reproductive success; single cell proteins; transcriptome sequencing; trial design

Project Summary The over-arching goal of this Phase II SBIR proposal is to optimize and implement high quality standard reference diets for the Zebrafish (Danio rerio), an animal model of critical importance to the understanding of human health and development of vertebrate organisms. A key problem in the industry is that high quality standardized reference diets, with appropriate physical properties and adequate feed management strategies, have not been developed for D. rerio. Our solution for the industry, driven by a synergistic collaboration between Meridian Biotech and the laboratory of Dr. Stephen Watts at the University of Alabama at Birmingham, is to develop several open formulation diets for larval, juvenile and adult Zebrafish, which will achieve a desired level of control and whereby use of this modern and effective biomedical animal model is substantially improved and more standardized. This commercial-academic collaboration is a significant value add to this applied science program. Key ingredients in this formulated diet will include protein packages that contain defined and effective sources, including a microbial based Single Cell Protein (SCP), an enriched protein source obtained from bacterial biomass. Diets with these protein sources have been developed by Meridian Biotech, several of which have improved shrimp and fish farming feed technologies markedly; thus, we wish to apply this technology to D. rerio and, potentially, other relevant animal models. Our mutual Phase II Milestones and Specific Experimental Aims are many across both years of the program. Specific Aims for Milestone 1 (Year 1 work) include optimizing the protein content and amino acid profile of our most promising ingredient and testing it against the existing SCP-fortified diet used in pilot Phase I work, comparing the best diet to the competitor diets currently used in academic cores across the US biomedical research enterprise, and characterizing deeply at the phenotypic and genetic levels the benefits of our diet. Milestone 1 will seek to identify and progress the best diet and drive its market penetration, initially in academia. Specific Aims for Milestone 2 (Year 2 work) include further optimizing the amino acid, fatty acid, neutral fat, and energy of the SCP ingredient and scaling up manufacturing to meet demands at Meridian’s new US-based facilities, sharing the best diet formulation with academic Zebrafish cores to drive adoption of the diet, and centralizing the data gathering from the many academic cores for use by all and the NIH ORIP. Milestone 2 will seek to penetrate the academic Zebrafish husbandry marketplace by gaining Core adoption. In parallel with collaborative efforts within the academic marketplace, Meridian and commercial partners across both years will also pursue a Milestone 3 which seeks to expand the technologies and penetrate the industry fish aquaculture and farming marketplace, other production species, and potentially develop human applications.

项目概要 第二阶段 SBIR 提案的首要目标是优化和实施高质量标准 斑马鱼（Danio rerio）的参考饮食，这是一种对于理解斑马鱼至关重要的动物模型 人类健康和脊椎动物发展的一个关键问题是高质量。 标准化参考日粮，具有适当的物理特性和适当的饲料管理策略， 尚未针对斑马鱼开发我们的行业解决方案，由协同合作驱动。 Meridian Biotech 与阿拉巴马大学 Stephen Watts 博士实验室合作 伯明翰将为斑马鱼幼虫、幼鱼和成年斑马鱼开发几种开放式配方饲料，这将 达到理想的控制水平，从而使用这种现代有效的生物医学动物模型 这种商业学术合作具有重大价值。 添加到这个应用科学计划中的关键成分将包括蛋白质包。 含有明确且有效的来源，包括基于微生物的单细胞蛋白（SCP），一种丰富的 由细菌生物质获得的蛋白质来源已由以下公司开发。 Meridian Biotech，其中一些公司显着改进了虾和鱼养殖饲料技术； 我们希望将这项技术应用于斑马鱼，并可能应用于我们共同的第二阶段动物模型。 该计划的这两年的具体目标有很多里程碑和具体实验目标。 里程碑 1（第一年的工作）包括优化我们最有前途的蛋白质含量和氨基酸谱 成分并与第一阶段试点工作中使用的现有 SCP 强化饮食进行测试，比较最佳的 与目前美国生物医学研究企业学术核心中使用的竞争对手饮食相比， 并在表型和基因水平上深入描述我们饮食的好处。 确定并改进最佳饮食并推动其市场渗透，首先是在学术界的具体目标。 里程碑 2（第 2 年工作）包括进一步优化氨基酸、脂肪酸、中性脂肪和能量 SCP 成分并扩大生产规模以满足 Meridian 位于美国的新工厂的需求，分享 具有学术斑马鱼核心的最佳饮食配方，以推动饮食的采用，并集中数据 从许多学术核心中收集供所有人使用，NIH ORIP 将寻求渗透。 通过获得核心采用以及协作努力，学术斑马鱼饲养市场。 在学术市场中，Meridian 和商业合作伙伴在这两年也将追求 里程碑 3 寻求扩展技术并渗透到鱼类水产养殖和养殖行业 市场、其他生产物种，并有可能开发人类应用。