Johns Hopkins Human Temporal Bone Resource

约翰霍普金斯大学人类颞骨资源

基本信息

批准号：
10705769
负责人：
John Patrick Carey
金额：
$ 79.24万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-16 至 2027-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10705769
关键词：
2019-nCoV 3-Dimensional Academic Medical Centers Acceleration Address Adopted Affect African American population Age Aging Animal Disease Models Arachnoid mater Auditory Autopsy Baltimore Biological Brain Brain Stem Catalogs Childhood Clinical Cochlea Collaborations Collection Communication Communities Consent Data Databases Decalcification Democracy Disease Ear Ear Diseases Education Education and Outreach Educational Materials Enrollment Ensure Equilibrium Equipment Facial nerve structure Faculty Funding Goals Harvest Health Hearing Hearing problem Hematoxylin and Eosin Staining Method Histology Human Image Improve Access Information Dissemination Infrastructure Intention Knowledge Laboratories Laboratory Finding Laboratory Research Labyrinth Longitudinal Studies Measures Medical Medical Illustration Melanins Meniere&apos s Disease Mentors Modernization Nature Neurosciences Online Systems Otology Participant Pathology Pattern Persons Population Heterogeneity Population Study Postdoctoral Fellow Presbycusis Prevalence Process Proliferating Protocols documentation Recording of previous events Research Research Personnel Resources Role Scientist Semicircular canal structure Sickle Cell Anemia Slide Specimen Stains Students Techniques Temporal bone structure Testing Time Tissues Training Uncertainty Underrepresented Populations Underrepresented Students Visualization Work analysis pipeline bilingualism cost crowdsourcing demographics digital education resources epidemiology study equilibration disorder imaging platform inner ear diseases macula meetings microCT nerve supply nonhuman primate novel otoconia outreach pre-doctoral preservation programs rapid technique reconstruction recruit response sex timeline tool training opportunity transcriptomics working group

项目摘要

Project Summary Temporal bone research is essential for refining our understanding of the mechanisms of otologic disease; however, the difficulty in accessing this tissue, the typically long processing times, and the expense have contributed to a dwindling number of labs engaged in this work. In the 1920s, the first human temporal bone laboratory in the U.S. was established at Johns Hopkins. The collection contains approximately ~1400 processed specimens from donors with a range of otologic diseases that were obtained primarily in the 1920s through 1970s, but only a handful of studies have made use of this collection in the past 20 years. We propose to make the collection more accessible to the scientific community and to re-establish the infrastructure for harvesting, imaging, and processing new specimens with the intention of recruiting new donors from population-based studies of aging in which participants have already consented to autopsies and brain donations being performed at Johns Hopkins, as well as other clinical otological studies ongoing in the Baltimore Metro Region. We will partner with faculty specializing in biological visualization, bilingual scientific communication, and mentoring diverse scientists to develop inclusive outreach tools. We will also partner with Baltimore-based transcriptomics resources to establish protocols for performing spatial transcriptomics analysis in nonhuman primate and human temporal bones. We will approach these objectives with three Specific Aims: 1) Catalog, prioritize, and digitize existing specimens from the Johns Hopkins Human Temporal Bone Collection; 2) Develop efficient harvesting, rapid histology, and spatial transcriptomics workflows for new specimens and establish partnerships for collecting specimens during autopsies performed on participants in epidemiological studies; 3) Education/outreach activities to encourage other investigators to engage in temporal bone research. The large temporal bone collection and the inner ear illustrations of Max Brödel at Johns Hopkins contributed greatly to our early understanding of many diseases of the ear, such as the discovery of glomus bodies, the histopathologic correlates of presbycusis, and innervation of the saccular maculae. The collection has also shown enduring value, contributing to our understanding of the prevalence of a dehiscent superior semicircular canal and arachnoid granulations as well as the role of melanin in cochlear and vestibular protection. Sharing this valuable collection with other researchers while applying novel techniques in digital processing and education will accelerate advances in and knowledge of otopathology. We look forward to reinvigorating this tradition of excellence by developing a new collection, applying modern techniques, and integrating otopathology with population-based studies that include comprehensive otologic testing and functional measures.

项目概要颞骨研究对于加深我们对耳科疾病机制的理解至关重要。然而，获取这种组织的困难、通常较长的处理时间以及费用 20 世纪 20 年代，第一块人类颞骨诞生，导致从事这项工作的实验室数量不断减少。美国约翰·霍普金斯大学实验室建立了大约 1400 个加工品。来自患有一系列耳科疾病的捐赠者的标本主要是在 20 年代通过 20 世纪 70 年代，但在过去 20 年里只有少数研究利用了这个集合。科学界更容易获得这些藏品并重建收集基础设施，成像和处理新标本，目的是从基于人群的人群中招募新的捐赠者已经同意进行尸检和大脑捐赠的衰老研究约翰·霍普金斯大学以及巴尔的摩都市区正在进行的其他临床耳科研究。与生物可视化、双语科学交流和指导方面的特殊教师合作我们还将与巴尔的摩的转录组学合作，开发包容性的外展工具。建立在非人类灵长类动物和人类中进行空间转录组学分析的协议的资源我们将通过三个具体目标来实现这些目标：1）编目、优先排序和数字化。约翰·霍普金斯大学人类颞骨收藏中的现有标本 2) 开发高效采集；新标本的快速组织学和空间转录组学工作流程，并建立收集合作伙伴关系对流行病学研究参与者进行尸检时的标本；3) 教育/推广；鼓励其他研究人员参与颞骨研究的活动。约翰·霍普金斯大学的马克斯·布罗德尔 (Max Brödel) 的大量颞骨收藏和内耳插图做出了贡献极大地促进了我们对许多耳朵疾病的早期认识，例如血管球体的发现、老年性耳聋和囊状黄斑神经支配的组织病理学相关性。显示出持久的价值，有助于我们了解上半圆形开裂的普遍性耳蜗和蛛网膜颗粒以及黑色素在耳蜗和前庭保护中的作用。这些有价值的收藏与其他研究人员同时应用数字处理和新技术教育将加速耳病理学的发展和知识的发展，我们期待着重振这一领域。通过开发新系列、应用现代技术并整合耳病理学来传承卓越传统基于人群的研究，包括全面的耳科测试和功能测量。