Spinning Disk Super Resolution Confocal Microscope for the Vanderbilt Brain Institute

范德比尔特脑研究所的转盘超分辨率共焦显微镜

基本信息

批准号：
10721222
负责人：
Jenny Schafer
金额：
$ 59.78万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-01 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10721222
关键词：
3-Dimensional Academic Medical Centers Aggressive behavior Area Attention deficit hyperactivity disorder Award Basic Science Brain Cells Communication Computers Core Facility Dedications Equipment Faculty Fees Financial Support Funding Image Impulsivity Incubated Institution Interdisciplinary Study Investigation Lasers Learning Medical Research Memory Mental Health Microscope Microscopy Minor Molecular National Institute of Mental Health Neurosciences Organoids Population Recording of previous events Research Research Institute Research Personnel Resolution Resource Sharing Rett Syndrome Sampling Signal Transduction Social Interaction Specialist Speed Synapses System Tissues Training United States National Institutes of Health Universities Wages cellular imaging confocal imaging instrument lens live cell imaging microscopic imaging new technology operation superresolution imaging synaptogenesis ultra high resolution

项目摘要

PROJECT SUMMARY / ABSTRACT Funds are requested to purchase a Nikon Spinning Disk Super Resolution (CSU-W1/SoRa) Confocal microscope for imaging projects focused around mental health research within the Vanderbilt Brain Institute (VBI), a multi-disciplinary research institute comprised of faculty at Vanderbilt University (VU) and Vanderbilt University Medical Center (VUMC). The requested instrument will be integrated into and managed by the Vanderbilt Cell Imaging Shared Resource (CISR), a microscopy core with a 20+ year history of successful microscopy management, training, and acquisition of new technology. This instrument capable of confocal imaging and super resolution imaging in live samples is ideal for 6 Major and 7 Minor Users within the VBI with projects surrounding synaptogenesis, synapse assembly and plasticity, subsynaptic signaling, and communication between cell and tissue populations. These molecular and cellular investigations will advance already funded NIMH and NIH projects totaling over $6.8M in FY22 in the areas of social interaction, impulsive aggression, learning and memory, Rett syndrome (RTT), ADHD, and more. The proposed microscope configuration is based on the needs of the VBI and Major Users for live imaging at cellular and subcellular resolution. The Nikon CSU-W1 SoRa confocal microscope includes: 1) CSU-W1 spinning disk plus Yokogawa SoRa super resolution spinning disk for super resolution near 120 nm in XY; 2) stage-top incubation for imaging live cells and organoids; 3) Z-Piezo stage for increased speed in Z for 3D volumes; 4) Hamamatsu Orca-Fusion sCMOS camera with 30-100 frames per second; 5) excitation lasers of 405, 488, 561, and 633 nm; and 6) objective lenses for live cell imaging at high resolution ranging from 10x, 20x, 40x, 60x, and 100x. This new system would be housed in existing CISR space in Medical Research Building III (MRBIII) where the VBI is based and the majority of the Major User labs are located. Vanderbilt University is providing financial support to promote the long-term operation of the instrument. If awarded, the Office of the Dean of Basic Sciences is committed to providing $75,000 for CISR staff salary, computer purchases and upgrades, and reduction in user fees over the first 5 years. Technical support and training of new users will be facilitated by the exceptional staff within the CISR, which has a greater than 20-year history of assisting over 300 labs on campus. Moreover, this equipment will become part of the Vanderbilt Nikon Center of Excellence (COE) that is housed within the CISR. This CISR/Nikon COE provides a dedicated local team of specialists to support the microscopy needs of investigators on campus. Collectively, the proposed confocal system, together with outstanding institutional and core facility support, will enable the long-term objective of supporting and advancing neuroscience and mental health research Vanderbilt.

项目概要/摘要需要资金购买尼康转盘超分辨率 (CSU-W1/SoRa) 共焦范德比尔特大脑研究所内专注于心理健康研究的成像项目显微镜 (VBI)，一个由范德比尔特大学 (VU) 和范德比尔特大学教师组成的多学科研究机构大学医学中心（VUMC）。所请求的工具将被集成到并由其管理范德比尔特细胞成像共享资源 (CISR)，具有 20 多年成功历史的显微镜核心显微镜管理、培训和新技术的获取。该仪器能够进行共焦活体样本成像和超分辨率成像非常适合 VBI 内的 6 个主要用户和 7 个次要用户围绕突触发生、突触组装和可塑性、突触亚信号传导的项目，以及细胞和组织群体之间的通讯。这些分子和细胞研究将推进 2022 财年，NIMH 和 NIH 项目在社交互动、冲动等领域的资助总额已超过 680 万美元攻击性、学习和记忆、雷特综合征 (RTT)、多动症等。提议的显微镜配置基于 VBI 和主要用户对实时成像的需求细胞和亚细胞分辨率。 Nikon CSU-W1 SoRa 共焦显微镜包括：1) CSU-W1 旋转盘加上横河 SoRa 超分辨率旋转盘，可实现 XY 近 120 nm 的超分辨率； 2）用于活细胞和类器官成像的台顶孵化； 3) Z-压电平台可提高 3D Z 轴的速度卷； 4）滨松Orca-Fusion sCMOS相机，每秒30-100帧； 5) 激发激光器 405、488、561 和 633 纳米； 6) 用于高分辨率活细胞成像的物镜，范围为 10x、20x、 40 倍、60 倍和 100 倍。该新系统将安置在医学研究三号楼现有的 CISR 空间内 (MRBIII) VBI 所在地和大多数主要用户实验室所在地。范德比尔特大学正在提供财政支持，以促进该仪器的长期运行。如果基础科学学院院长办公室承诺为 CISR 员工提供 75,000 美元的工资，计算机购买和升级，以及前 5 年内使用费的减少。技术支持和 CISR 内的杰出工作人员将协助新用户的培训，该机构拥有超过 20 年的经验协助校园内 300 多个实验室的历史。而且，该设备将成为范德比尔特尼康的一部分卓越中心 (COE) 位于 CISR 内。该 CISR/尼康 COE 提供了专用的本地专家团队支持校园研究人员的显微镜需求。总的来说，拟议的共聚焦系统，加上出色的机构和核心设施支持，将使长期支持和推进神经科学和心理健康研究范德比尔特的目标。