Development of Specializations Required for Temporal Coding in Octopus Cells

章鱼细胞时间编码所需专业化的发展

• 批准号: 10686066
• 负责人: Lauren J Kreeger
• 金额: $ 7.61万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10686066
• 关键词: Acoustic Nerve; Acoustics; Action Potentials; Acute; Adult; Anatomy; Animals; Auditory; Auditory Brain Stem Implants; Auditory system; Awareness; Binding; Biological Models; Biophysics; Brain; Brain Stem; Cell Extracts; Cell physiology; Cells; Characteristics; Child; Cochlea; Cochlear Implants; Cochlear nucleus; Code; Communication; Comprehension; Continuing Education; Cues; Data; Dendrites; Detection; Development; Distal; Ear; Electrophysiology (science); Endowment; Environment; Fire - disasters; Frequencies; Glean; Goals; Hearing; Image Analysis; Ion Channel; Ion Channel Gating; Leadership; Mammalian Cell; Maps; Measures; Mentorship; Methods; Modeling; Molecular Analysis; Molecular Genetics; Morphology; Mus; Nervous System; Neurobiology; Neurons; Octopus; Pathway interactions; Patients; Pattern; Perception; Phase; Physiological; Play; Population; Preparation; Property; Reporting; Research; Research Training; Role; Sensory; Slice; Speech; Speech Perception; Stimulus; Synapses; System; Time; Training; auditory pathway; biophysical analysis; biophysical properties; career; detection platform; developmental neurobiology; experience; experimental study; feature extraction; improved; insight; medical schools; nerve supply; neuron development; neuronal cell body; neuronal circuitry; normal hearing; parallel processing; sensory mechanism; sensory system; skills; sound; voltage

Project Summary The auditory system is an ideal place to study how neurons and circuits develop to encode sensory features with temporal precision. In the cochlear nucleus, specialized cells extract information about the acoustic world and initiate parallel processing pathways, each of which analyzes and encodes continuous auditory cues such as frequency, phase, and amplitude. The octopus cell of the mammalian cochlear nucleus stands out for its unique sensitivity to both the temporal and frequency components of sound stimuli. However, the mechanisms underlying development of tonotopically organized somatic and dendritic synapses in single neurons is not understood. Additionally, there is little understanding of the role of auditory experience in the refinement of morphological and physiological properties that contribute to temporal coding. I propose to carry out an integrated analysis of the molecular and biophysical properties of octopus cells and to track how features develop during the onset of auditory experience. Using the mouse as a model system, I will define the refinement of tonotopically organized inputs to octopus cell somas and dendrites before and after hearing onset while parallel changes in the size and complexity of the dendritic arbor occur. This data will provide insights into the role of auditory experience on the development of brainstem auditory circuits. I will also measure correlated changes in ion channel expression with changes in biophysical properties at developmental timepoints and measure electrophysiological changes as auditory experience first begins. These experiments will bridge the sequence of anatomical refinements with physiological refinements during hearing onset. The research training plan will provide extensive training in developmental neurobiology, methods in mouse molecular genetics, quantitative approaches, and computational image analysis. Training will occur in the rich scientific environment at Harvard Medical School in the Department of Neurobiology. Additionally, the training plan includes continuing education in mentorship, communication, management, diversity, and leadership. The training provided under this plan will provide essential skills for a successful independent research career.

项目概要 听觉系统是研究神经元和电路如何发育以编码感觉特征的理想场所 时间精度。在耳蜗核中，专门的细胞提取有关声音世界的信息并 启动并行处理路径，每个路径都分析和编码连续的听觉线索，例如 频率、相位和幅度。哺乳动物耳蜗核的章鱼细胞因其独特的功能而脱颖而出 对声音刺激的时间和频率分量的敏感性。然而，这些机制 单个神经元中按音调组织的体细胞和树突突触的潜在发育并不 明白了。此外，人们对听觉体验在完善听觉体验中的作用知之甚少。 有助于时间编码的形态和生理特性。 我建议对章鱼细胞的分子和生物物理特性进行综合分析，并 跟踪听觉体验开始期间特征如何发展。使用鼠标作为模型系统，我将 定义前后对章鱼细胞体和树突的音调组织输入的细化 当树突乔木的大小和复杂性发生平行变化时，听力开始发生。该数据将提供 深入了解听觉体验对脑干听觉回路发育的作用。我也会测量 离子通道表达的变化与发育时间点生物物理特性的变化相关 并测量听觉体验开始时的电生理变化。这些实验将弥补 听力出现期间的解剖学改进和生理学改进的顺序。 研究培训计划将提供发育神经生物学、小鼠方法方面的广泛培训 分子遗传学、定量方法和计算图像分析。培训将在富人中进行 哈佛医学院神经生物学系的科学环境。此外，培训 计划包括指导、沟通、管理、多样性和领导力方面的继续教育。这 该计划提供的培训将为成功的独立研究生涯提供基本技能。

项目成果

An Anatomical and Physiological Basis for Coincidence Detection Across Time Scales in the Auditory System.

听觉系统中跨时间尺度的重合检测的解剖学和生理学基础。

• 发表时间: 2024-02-29
• 作者: Kreeger, Lauren J;Honnuraiah, Suraj;Maeker, Sydney;Shea, Siobhan;Fishell, Gord;Goodrich, Lisa V
• 通讯作者: Goodrich, Lisa V