Organ Specific Project - Heart

器官特定项目 - 心脏

• 批准号: 10530970
• 负责人: Liming Pei
• 金额: $ 156.91万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10530970
• 关键词: ATAC-seq; Address; Affect; Age; Aging; Algorithms; American; Anatomy; Archives; Arteries; Atlases; Basic Science; Biological Assay; Biology; Biomedical Research; Cardiac; Cause of Death; Cell Nucleus; Cells; Chromatin; Clinical; Communities; Computing Methodologies; Consent Forms; Data; Data Analyses; Databases; Detection; Development; Epigenetic Process; Ethnic group; Female; Fluorescent in Situ Hybridization; Follow-Up Studies; Freezing; Future; Gender; Genetic Transcription; Genomics; Goals; Growth; Health; Heart; Heart Diseases; Heterogeneity; Histologic; Human; Human BioMolecular Atlas Program; Image; Infrastructure; Knowledge; Limes; Longevity; Medical; Metadata; Methods; Molecular; Molecular Profiling; Multiomic Data; Organ; Phase; Physiological; Physiology; Positioning Attribute; Production; Proteomics; Race; Regulatory Pathway; Research; Resolution; Sampling; Series; Signal Pathway; Small Nuclear RNA; Spatial Distribution; Specimen; Three-Dimensional Imaging; Tissues; Transposase; United States; Veins; Visualization; Woman; age group; base; biobank; cell type; complex data; computational pipelines; computerized tools; cost; data sharing; epigenomics; experience; genomic data; heart cell; improved; indexing; male; men; microCT; molecular imaging; multiple omics; searchable database; serial imaging; single cell analysis; transcriptome sequencing; transcriptomics; user-friendly

PROJECT SUMMARY – HEART OSP The human heart is vital for our survival and health, and it presents remarkable anatomical, cellular and functional heterogeneity. The four chambers of the heart, together with specialized arteries, veins, valves and conduction cells, perform distinct yet essential physiological functions. A significant gap of knowledge is that different cell’s molecular signature, spatial distribution and interactions, and functional state remain little understood at the single-cell level. The goal of the heart Organ-Specific Project (OSP) is to address this knowledge gap and generate high quality, single-cell resolution, longitudinal imaging and multiomics data of normal human hearts across the entire human lifespan. To achieve this goal, we will generate: 1) Human heart atlases. Spatially resolved atlases will provide a highly user friendly, publicly available, searchable database of the most comprehensive multi-omic, single cell analysis of the normal human hearts. Molecular data will be richly annotated with additional clinical and demographic data. 2) Computational methods. In addition to the atlas data, the critical computational tools and pipelines developed in this project will be available to the research community. These include methods and pipelines for processing multi-omics and imaging data, inference of cell- specific regulatory and signaling pathways, correlation of mesoscale imaging and molecular imaging features, as well as database algorithms for the query, exploration and visualization of highly complex data. 3) Access to biospecimens for follow-up studies. Biospecimens collected in this project will be banked and made available to the biomedical research community. These include freshly frozen and fixed specimens and tissue sections. In summary, the heart OSP will broadly impact the entire research community and jumpstart basic-science and medical discoveries based on a sophisticated understanding of the key molecular circuits underlying the development and aging of human heart.

项目摘要 - 心脏OSP 人的心脏对我们的生存和健康至关重要，它表现出显着的解剖学，细胞和功能 异质性。心脏的四个腔室以及专门的动脉，静脉，瓣膜和传导 细胞，执行不同但必不可少的生理功能。一个很大的知识差距是不同的单元格 分子特征，空间分布和相互作用以及功能状态在 单细胞水平。心脏器官特定项目（OSP）的目标是解决此知识差距和 产生高质量的单细胞分辨率，正常人心脏的纵向成像和多组合数据 在整个人类的寿命中。为了实现这一目标，我们将产生：1）人类心脏地图集。空间 已解决的地图集将提供最友好的，公共可用的，可搜索的数据库 正常人心脏的综合多摩变，单细胞分析。分子数据将是丰富的 用其他临床和人口统计数据注释。 2）计算方法。除了地图集数据外， 该项目中开发的关键计算工具和管道将用于研究 社区。这些包括用于处理多词和成像数据的方法和管道，细胞的推断 特定的调节和信号通路，中尺度成像和分子成像特征的相关性， 以及用于查询，高度复杂数据的查询，探索和可视化的数据库算法。 3）访问 生物测量进行后续研究。该项目中收集的双粒子将被存放并提供给 生物医学研究界。这些包括新鲜冷冻和固定的标本和组织切片。在 总而言之，心脏OSP将广泛影响整个研究界和启动基本科学和 基于对关键分子电路的复杂理解的医学发现 人心的发展和衰老。