Organ Specific Project - Heart

器官特定项目 - 心脏

基本信息

批准号：
10251352
负责人：
Liming Pei
金额：
$ 5.96万
依托单位：
CHILDREN'S HOSP OF PHILADELPHIA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2022-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10251352
关键词：
3-Dimensional Address Age Aging Anatomy Archives Arteries Atlases Basic Science Biological Assay Biology Bone Tissue Cardiac Cell Nucleus Cells Chromatin Clinical Collection Communities Consent Forms Data Data Analyses Detection Development Doctor of Medicine Doctor of Philosophy Epigenetic Process Ethnic Origin Female Fluorescent in Situ Hybridization Future Genetic Transcription Genomics Goals Growth Health Heart Heterogeneity Histologic Hour Human Human BioMolecular Atlas Program Image Infrastructure Knowledge Leadership Limes Location Longevity Medical Metadata Molecular Molecular Profiling Multiomic Data Organ Organ Procurements Persons Physiological Pilot Projects Proteomics Protocols documentation Research Research Personnel Resolution Sampling Series Small Nuclear RNA Spatial Distribution Specimen Three-Dimensional Imaging Tissue Banks Transposase Veins age group base biobank bone cell type data sharing design epigenome experience genomic data indexing inter-individual variation male member metabolomics microCT multiple omics serial imaging sex transcriptome transcriptome sequencing transcriptomics

项目摘要

PROJECT SUMMARY – HEART ORGAN SPECIFIC PROJECT The human heart is vital for our survival and health, and it presents remarkable anatomical, cellular and functional heterogeneity. The four chambers of the heart, together with specialized arteries, veins, valves and conduction cells, perform distinct yet essential physiological functions. A significant gap of knowledge is that different cell's molecular signature, spatial distribution and interactions, and functional state remain little understood at the single-cell level. The goal of the heart Organ-Specific Project (OSP) is to address this knowledge gap and generate high quality, single-cell resolution, longitudinal imaging and multiomics data of normal human hearts across the entire human lifespan. To achieve this goal, we propose the following three specific aims: 1) To refine protocols of biospecimen processing, multiomics and imaging assays and define inter-individual variability using our existing banked normal human hearts. 2) To procure, archive and annotate high-quality normal heart samples across the entire human life span. We have established a streamlined procurement and biorepository infrastructure to support our heart OSP. We will procure normal heart and bone from the same donor of 5 different age groups across the entire human life span. 3) To spatially and quantitatively profile normal heart specimens across the entire human lifespan using a set of robust and scalable imaging and single-cell omics assays. In summary, the heart OSP will broadly impact the entire research community and jumpstart basic-science and medical discoveries based on a sophisticated understanding of the key molecular circuits underlying the development and aging of human heart.

项目摘要 – 心脏器官特定项目人类心脏对于我们的生存和健康至关重要，它具有非凡的解剖学、细胞和功能。心脏的四个腔室，以及专门的动脉、静脉、瓣膜和功能异质性。传导细胞执行独特但重要的生理功能。不同细胞的分子特征、空间分布和相互作用以及功能状态仍然很少心脏器官特异性项目（OSP）的目标就是解决这个问题。知识差距并生成高质量、单细胞分辨率、纵向成像和多组学数据为了实现这一目标，我们提出以下三项建议。具体目标： 1) 完善生物样本处理、多组学和成像分析的方案并定义使用我们现有的正常人类心脏进行个体间变异 2) 获取、存档和注释。我们建立了一个简化的流程，可以在整个人类生命周期中提供高质量的正常心脏样本。支持我们的心脏 OSP 的采购和生物储存基础设施我们将采购正常的心脏和骨骼。来自整个人类生命周期中 5 个不同年龄组的同一捐献者 3) 在空间和空间上。使用一组稳健且可靠的方法对整个人类生命周期中的正常心脏样本进行定量分析总之，心脏 OSP 将广泛影响整个领域。研究社区并基于复杂的基础科学和医学发现了解人类心脏发育和衰老的关键分子回路。