Organ Specific Project - Heart

器官特定项目 - 心脏

基本信息

批准号：
10251352
负责人：
Liming Pei
金额：
$ 5.96万
依托单位：
CHILDREN'S HOSP OF PHILADELPHIA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2022-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10251352
关键词：
3-Dimensional Address Age Aging Anatomy Archives Arteries Atlases Basic Science Biological Assay Biology Bone Tissue Cardiac Cell Nucleus Cells Chromatin Clinical Collection Communities Consent Forms Data Data Analyses Detection Development Doctor of Medicine Doctor of Philosophy Epigenetic Process Ethnic Origin Female Fluorescent in Situ Hybridization Future Genetic Transcription Genomics Goals Growth Health Heart Heterogeneity Histologic Hour Human Human BioMolecular Atlas Program Image Infrastructure Knowledge Leadership Limes Location Longevity Medical Metadata Molecular Molecular Profiling Multiomic Data Organ Organ Procurements Persons Physiological Pilot Projects Proteomics Protocols documentation Research Research Personnel Resolution Sampling Series Small Nuclear RNA Spatial Distribution Specimen Three-Dimensional Imaging Tissue Banks Transposase Veins age group base biobank bone cell type data sharing design epigenome experience genomic data indexing inter-individual variation male member metabolomics microCT multiple omics serial imaging sex transcriptome transcriptome sequencing transcriptomics

项目摘要

PROJECT SUMMARY – HEART ORGAN SPECIFIC PROJECT The human heart is vital for our survival and health, and it presents remarkable anatomical, cellular and functional heterogeneity. The four chambers of the heart, together with specialized arteries, veins, valves and conduction cells, perform distinct yet essential physiological functions. A significant gap of knowledge is that different cell's molecular signature, spatial distribution and interactions, and functional state remain little understood at the single-cell level. The goal of the heart Organ-Specific Project (OSP) is to address this knowledge gap and generate high quality, single-cell resolution, longitudinal imaging and multiomics data of normal human hearts across the entire human lifespan. To achieve this goal, we propose the following three specific aims: 1) To refine protocols of biospecimen processing, multiomics and imaging assays and define inter-individual variability using our existing banked normal human hearts. 2) To procure, archive and annotate high-quality normal heart samples across the entire human life span. We have established a streamlined procurement and biorepository infrastructure to support our heart OSP. We will procure normal heart and bone from the same donor of 5 different age groups across the entire human life span. 3) To spatially and quantitatively profile normal heart specimens across the entire human lifespan using a set of robust and scalable imaging and single-cell omics assays. In summary, the heart OSP will broadly impact the entire research community and jumpstart basic-science and medical discoveries based on a sophisticated understanding of the key molecular circuits underlying the development and aging of human heart.

项目摘要 - 心脏器官特定项目人心脏对我们的生存和健康至关重要，它表现出显着的解剖学，细胞和功能异质性。心脏的四个腔室以及专门的动脉，静脉，瓣膜和传导细胞，执行不同但必不可少的生理功能。一个很大的知识差距是不同细胞的分子特征，空间分布和相互作用以及功能状态几乎没有在单细胞级别上了解。心脏器官特定项目（OSP）的目标是解决这个问题知识差距并产生高质量，单细胞分辨率，纵向成像和多组学数据整个人类寿命的正常人心。为了实现这一目标，我们提出以下三个具体目的：1）完善生物测量处理，多组合和成像测定方案并定义使用我们现有的银行正常人心心脏的个体间变异性。 2）采购，存档和注释在整个人类生活范围内，高质量的正常心脏样本。我们已经建立了一个简化的采购和生物座席基础设施以支持我们的心OSP。我们将采取正常的心脏和骨头来自整个人类寿命的5个不同年龄段的同一捐助者。 3）在空间上和使用一组健壮的和可扩展成像和单细胞OMICS分析。总而言之，心脏OSP将广泛影响整个研究社区和基础科学和医学发现，基于复杂的了解人心发展和衰老的关键分子回路。