Fluorescence Imaging Facility

荧光成像设备

• 批准号: 7969940
• 负责人: james g mcnally
• 金额: $ 25.53万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7969940
• 关键词: Arts; Binding; CCR; Cells; Fluorescence Microscopy; Fluorescence Recovery After Photobleaching; Fluorescence Resonance Energy Transfer; Life; Measures; Mission; Receptor Biology and Gene Expression; Research Personnel; Sorting - Cell Movement; Specimen; Techniques; Time; Work; fluorescence imaging; instrumentation; light microscopy; research study; tool

The facility has two objectives. First, its mission is to provide state-of-the-art light microscopy instrumentation for the Lab of Receptor Biology and Gene Expression. Second, the facility provides support to anyone in the CCR who requires assistance with fluorescence imaging. Present work in the facility revolves around four different general techniques: structural studies such as colocalization of different fluorescent markers, time-lapse analysis to follow how cellular components change over time, fluorescence recovery after photobleaching to measure mobilities and binding of cell molecues and fluorescence resonance energy transfer to measure molecular interactions in live or fixed specimens.

该设施有两个目标。首先，其任务是为受体生物学和基因表达实验室提供最新的光显微镜仪器。其次，该设施为CCR中需要荧光成像协助的任何人提供了支持。该设施中的现有工作围绕四种不同的一般技术旋转：结构研究，例如不同荧光标记的共定位，延时分析，以遵循细胞成分随时间的变化，光漂白后的荧光恢复以测量迁移率以及测量细胞分子的结合和荧光能量的结合，以测量现场或固定表明中的分子相互作用，以测量分子相互作用。