Large Scale Chromatin Structure

大规模染色质结构

• 批准号: 7061471
• 负责人: james g mcnally
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7061471
• 关键词: DNA repair; DNA replication; chemical structure function; chromatin; genetic transcription; imaging /visualization /scanning; light microscopy; nucleic acid structure; protein folding; protein structure function; structural biology

Large-scale chromatin structure is the higher-order folding of chromatin on the scale of several hundred kilobases. Little is known about this folding, but the structures must influence transcription, replication and repair of DNA. To learn more about these structures, we have examined transcriptionally active domains of 400 - 2000 kb by light microscopy. We find that several different domains exhibit similar structures in light microscope images, namely a series of adjacent puncta or "beads" approximately 0.5 microns in diameter. When these same domains are transcriptionally inactive, only one punctum or "bead" is detected. Thus transcription induces a decondensation from single beaded to multi-beaded structures. These observations impose constraints on the higher order folding of chromatin. Present work focuses on identifying molecules associated with these structures, and observing the structures by higher resolution imaging.

大规模染色质结构是几百千倍酶的色质的高阶折叠。关于这种折叠知之甚少，但是结构必须影响DNA的转录，复制和修复。为了了解有关这些结构的更多信息，我们通过光学显微镜检查了400-2000 kb的转录活性域。我们发现，几个不同的域在光学显微镜图像中表现出相似的结构，即直径约0.5微米的一系列相邻点或“珠”。当这些相同的域在转录上无效时，仅检测到一个点击量或“珠”。因此，转录引起了从单个串珠到多珠结构的反应。这些观察结果对染色质的高阶折叠施加了约束。目前的工作着重于识别与这些结构相关的分子，并通过更高的分辨率成像观察结构。