Neuropeptide modulation of biogenic amine function and aggression in a crustacean

甲壳动物生物胺功能和攻击性的神经肽调节

• 批准号: 8245718
• 负责人: MARIA A SOSA
• 金额: $ 11.14万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8245718
• 关键词: Adult; Affect; Aggressive behavior; Amines; Animals; Astacoidea; Attitude; Behavior; Binding Sites; Biogenic Amines; Body Size; Cardiovascular system; Cells; Characteristics; Claw; Color; Confocal Microscopy; Crabs; Crustacea; Dissection; Elements; Environment; Equipment and supply inventories; Exposure to; FMRFamide; Female; Food; Fresh Water; Future; Genes; Goals; Health Status; Hemolymph; Immunohistochemistry; Injection of therapeutic agent; Invertebrates; Knowledge; Lead; Light; Link; Lobster; Macrobrachium; Mass Spectrum Analysis; Mental Health; Modeling; Morphology; Neuraxis; Neurons; Neuropeptides; Octopamine; Organism; Peptides; Perception; Play; Positioning Attribute; Regulation; Relative (related person); Reproduction; Research; Role; Sampling; Sensory; Serotonin; Shelter facility; Signal Transduction; Social Dominance; Social Hierarchy; Source; Staging; System; Techniques; Territoriality; Testing; Vertebrates; behavior observation; design; fighting; interest; male; neural circuit; novel; proctolin; public health relevance; research study; response

DESCRIPTION (provided by applicant): Neuropeptide modulation of biogenic amine function and aggression in a crustacean. The long-term goal of this project is to understand the mechanisms that control aggressive behavior in an invertebrate model and how the underlying neural circuits are modulated in response to signals from the organism's internal and external environment. The specific goal is to understand how the effects serotonin (5-HT) and octopamine (OA) have on aggressive behavior of dominant and submissive freshwater prawns are modulated by neuroactive peptides. Adult male prawns develop through three morphotypes, corresponding with the animal's status in a dominance hierarchy. The typical behaviors of each morphotype vary quite markedly in aggression, territoriality and attitude towards females. While other crustacean models such as the crab, crayfish and lobster, have been used extensively to study interactive behaviors, the prawn offers the advantage of marked differences in characteristic behaviors of each morphotype and the fact that the position each animal assumes in the hierarchy of dominance is first determined by its morphotype rather than by its body size. There is ample evidence linking biogenic amines to behaviors associated with the establishment of social hierarchies in both vertebrates and invertebrates, but the specific mechanisms by which these amines act remain largely unknown. Serotonin and OA have been shown to play an important role in modulating aggression and fighting behavior in crustaceans, including the prawn, but the evidence suggests that they are not the sole determinant elements. Aggression and fighting behavior can vary depending on circumstances such as availability of food, shelter and partners for reproduction, level of exposure to contaminants, light/dark cycles, health status, etc. The primary mechanisms that produce aggressive behavior are likely to be susceptible to diverse sources of modulation that allow the organism to adjust its responses to the prevailing circumstances. We are interested in studying the role played by neuroactive peptides in modulating the actions of biogenic amines and how this modulation results in changes in aggressive behaviors in the prawn. The specific scientific aims of the proposed research are to: (1) Characterize the role played by proctolin, FMRFamide, SIFamide and newly identified prawn neuropeptides in modulating natural aggressive behaviors of the prawn male morphotypes and aggressive behaviors induced by injection of 5-HT and OA; (2) Characterize the central nervous system (CNS) distribution of these neuropeptides in the prawn morphotypes and determine how it relates with those of 5-HT and OA; and (3) Quantify the relative amounts of these neuropeptides in the circulating hemolymph of the prawn morphotypes. To achieve these aims, we will use techniques of behavioral observation and quantitation before and after injections of agents of interest, CNS dissection, immunohistochemistry, confocal microscopy, hemolymph sampling and quantitative mass spectrometry. PUBLIC HEALTH RELEVANCE: Neuropeptide modulation of biogenic amine function and aggression in a crustacean. A thorough understanding of the functions proctolin, FMRFamide, SIFamide and other newly identified prawn peptides are likely to be playing in modulating the actions of CNS biogenic amines will help to dramatically expand the inventory of potential target molecules, binding sites, messenger systems and genes for controlling or regulating various forms of interactive behaviors and will provide new details regarding the components of the neural circuitry involved in the mechanisms underlying the regulation of aggressive behavior and how they may interact with one another. This knowledge will ultimately be of use in the future design of new forms of treatment for mental health and other CNS ailments related with these transmitter and modulatory systems.

描述（由申请人提供）：甲壳类动物中生物胺功能和侵略性的神经肽调节。该项目的长期目标是了解控制无脊椎动物模型中侵略行为的机制，以及如何根据有机体内部和外部环境的信号调节潜在的神经回路。具体的目标是了解5-羟色胺（5-HT）和章鱼胺（OA）如何对主导和服从性淡水虾的侵略行为受到神经活性肽的调节。成年雄性大虾通过三种形态型出现，与动物在优势等级中的地位相对应。每种形态的典型行为在侵略性，领土和对女性的态度上差异很大。尽管其他甲壳类模型（例如螃蟹，小龙虾和龙虾）已被广泛用于研究互动行为，但虾提供了每种形态的特征性行为明显差异的优势，而每种动物在优势层次中假定的位置首先由其形态型而不是体外大小确定。有足够的证据将生物胺与与脊椎动物和无脊椎动物建立社会等级相关的行为联系起来，但是这些胺作用的具体机制在很大程度上是未知的。血清素和OA已被证明在调节包括大虾在内的甲壳类动物的侵略和战斗行为方面起着重要作用，但证据表明它们不是唯一的决定因素。侵略性和战斗行为可能会取决于诸如食物的可用性，庇护所和伴侣的可用性，暴露于污染物的水平，光/黑暗周期，健康状况等。产生侵略性行为的主要机制可能容易受到各种调制源的影响，从而使生物体对其对盛行的反应进行了反应。我们有兴趣研究神经活性肽在调节生物胺的作用以及这种调节如何导致虾中侵略性行为变化的作用。拟议的研究的具体科学目的是：（1）表征前血管林，fmrfamide，sifamide和新鉴定的虾神经肽在调节虾雄性形态的自然侵略行为方面所扮演的角色，并通过5-HT和OA造成了攻击性行为； （2）表征这些神经肽在虾形型中的中枢神经系统（CNS）分布，并确定其与5-HT和OA的关系； （3）量化这些神经肽在大虾形型的循环血淋巴中的相对量。为了实现这些目标，我们将在注射感兴趣的药物，CNS解剖，免疫组织化学，共聚焦显微镜，血淋巴样采样和定量质谱法之前和之后使用行为观察和定量技术。 公共卫生相关性：甲壳类动物生物胺功能和侵略性的神经肽调节。对蛋白周期，FMRFAMIDE，SIFAMIDE和其他新确定的大虾肽的功能的透彻理解可能会在调节CNS生物生物胺的作用时发挥作用调节侵略性行为以及它们如何相互作用的机制。这些知识最终将在未来设计的精神健康治疗形式以及与这些发射机和调节系统有关的其他CNS疾病的设计中使用。