Contrasting environmental and genetic controls of alternative phenotypes

对比环境和遗传对替代表型的控制

• 批准号: 8116638
• 负责人: JENNIFER A BRISSON
• 金额: $ 24.88万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-08 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8116638
• 关键词: Address; Adult; Aphids; Biological Assay; Biological Models; Biology; Complex; Crowding; Cues; Development; Disease susceptibility; Environment; Exhibits; Female; Gas Chromatography; Gene Expression; Gene Expression Profile; Genes; Genetic; Genetic Polymorphism; Genetic Recombination; Genetic Variation; Genomics; Genotype; Goals; Height; Individual; Link; Maps; Mediating; Metabolic; Molecular Genetics; Morphology; Names; Nature; Pathway interactions; Phenotype; Pisum sativum; Population; Predisposition; Production; RNA Sequences; Research; Science; Signal Transduction; Stimulus; Study models; Systems Biology; Variant; Weight; Wing; asexual; base; developmental genetics; insight; male; metabolomics; response

The proposed research addresses a fundamental question in biology: how do the environment and genotype interact to produce the phenotype? The pea aphid, an emerging genomic model system, exhibits dramatically different adult phenotypes of winged or unwinged morphs that are induced by environmental conditions in asexual females (a polyphenism) and by a single unidentified genetic locus in males (a polymorphism). Further, genetic variation for the female wing polyphenism segregates with the male wing polymorphism suggesting that the developmental networks underlying the polyphenism and polymorphism are not independent. I propose to (1) map and identify the male polymorphism gene to determine the developmental genetic basis of the male polymorphism, (2) characterize the transcriptional and metabolomic response to wing-inducing stimuli in the asexual females to determine the developmental genetic basis of the female polyphenism and (3) use a systems biology approach to link the results from (1) and (2) to provide a cohesive picture of how environmental signals and genetic signals interact to produce fundamentally similar adult alternative morphologies.

拟议的研究解决了生物学中的一个基本问题：环境和基因型如何相互作用以产生表型？ PEA蚜虫是一种新兴的基因组模型系统，在无性女性的环境条件（一种多态性）和男性（多态性）中单个未识别的遗传基因座（一种多态性的遗传基因座）（一种多态性）表现出巨大不同的成年成人表型。此外，雌性翼多态性的遗传变异与男性翼多态性分离，表明多形和多态性的发育网络并非独立。 I propose to (1) map and identify the male polymorphism gene to determine the developmental genetic basis of the male polymorphism, (2) characterize the transcriptional and metabolomic response to wing-inducing stimuli in the asexual females to determine the developmental genetic basis of the female polyphenism and (3) use a systems biology approach to link the results from (1) and (2) to provide a cohesive picture of how environmental信号和遗传信号相互作用以产生根本相似的 成人替代形态。

项目成果

Accelerated evolution of morph-biased genes in pea aphids.

• DOI: 10.1093/molbev/msu149
• 发表时间: 2014-08
• 期刊: Molecular biology and evolution
• 影响因子: 10.7
• 作者: Swapna R. Purandare;Ryan D. Bickel;J. Jaquiéry;C. Rispe;J. Brisson

Functional conservation of DNA methylation in the pea aphid and the honeybee.

• DOI: 10.1093/gbe/evq057
• 发表时间: 2010
• 期刊: Genome biology and evolution
• 影响因子: 3.3
• 作者: Hunt BG;Brisson JA;Yi SV;Goodisman MA
• 通讯作者: Goodisman MA

Comparison of the wing polyphenic response of pea aphids (Acyrthosiphon pisum) to crowding and predator cues.

• DOI: 10.1111/een.12080
• 发表时间: 2014-04-01
• 期刊: Ecological entomology
• 影响因子: 2.2
• 作者: Purandare SR;Tenhumberg B;Brisson JA
• 通讯作者: Brisson JA

The genomewide transcriptional response underlying the pea aphid wing polyphenism.

• DOI: 10.1111/mec.13749
• 发表时间: 2016-09
• 期刊: Molecular ecology
• 影响因子: 4.9
• 作者: Vellichirammal NN;Madayiputhiya N;Brisson JA
• 通讯作者: Brisson JA

Aphids: a model for polyphenism and epigenetics.

• DOI: 10.1155/2012/431531
• 发表时间: 2012
• 期刊: Genetics research international
• 作者: Srinivasan DG;Brisson JA
• 通讯作者: Brisson JA