Genomic analysis of wing dimorphism in pea aphids

豌豆蚜翅二态性的基因组分析

• 批准号: 7682645
• 负责人: JENNIFER A BRISSON
• 金额: $ 0.2万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-15 至 2008-09-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7682645
• 关键词: Alleles; Aphids; Biological Models; Biology; Candidate Disease Gene; Cues; Data; Depth; Development; Female; Gene Expression; Genes; Genetic; Genomics; Insecta; Mothers; Numbers; Pathway interactions; Pisum; Pisum sativum; Plants; Research; Research Personnel; Staging; Stimulus; Testing; Wing; X Chromosome; base; cDNA Arrays; cDNA Library; developmental genetics; dimorphism; insight; male; research study; response; Alleles; Aphids; Biological Models; Biology; Candidate Disease Gene; Cues; Data; Depth; Development; Female; Gene Expression; Genes; Genetic; Genomics; Insecta; Mothers; Numbers; Pathway interactions; Pisum; Pisum sativum; Plants; Research; Research Personnel; Staging; Stimulus; Testing; Wing; X Chromosome; base; cDNA Arrays; cDNA Library; developmental genetics; dimorphism; insight; male; research study; response

DESCRIPTION (provided by applicant): The aim of the proposed research is to use a genomic approach to generate a deeper understanding of the biology of winged morph induction and development in an emerging genomic model system, the pea aphid (Acyrthosiphon pisum). In this species, normally wingless asexually reproducing females produce winged offspring in response to environmental stimuli such as overcrowding or poor host plant quality. In contrast, males are winged or unwinged depending on the allele they carry of a gene on the X chromosome. The specific aims are to use pea aphid cDNA microarrays to i) examine the changes in gene expression that result from exposure of aphid mothers to wing-inducing cues and ii) examine changes in gene expression that accompany the development of the winged and unwinged morphs of both females and males. These experiments will generate hypotheses about which genetic pathways are involved in the i) induction and ii) development and maintentance of winged and unwinged morphs. These hypotheses will then be tested by examination of candidate genes using traditional approaches. Overall, experiments in the proposed research will provide insight into the developmental genetic basis of extreme phenotypic plasticity.

描述（由申请人提供）：拟议研究的目的是使用基因组方法来更深入地了解新兴的基因组模型系统中的有翼变体诱导和发育的生物学，PEA蚜虫（Acyrthosiphon Pisum）。在该物种中，通常无翼的无性繁殖女性会响应环境刺激（例如人满为患或宿主植物质量差）产生有翼的后代。相比之下，雄性根据X染色体上的基因携带的等位基因而被翅膀或不愿意。具体的目的是使用豌豆蚜虫cDNA微阵列i）检查蚜虫母亲暴露于翅膀引起的提示引起的基因表达的变化，ii）检查基因表达的变化，这些基因表达的变化伴随着女性和男性的有翼和未知的形态的发展。这些实验将产生关于i）诱导和ii）有翼和不情愿的形态的遗传途径的假设。然后，将使用传统方法检查候选基因来测试这些假设。总体而言，拟议的研究中的实验将为极端表型可塑性的发育遗传基础提供深入的了解。