Contrasting environmental and genetic controls of alternative phenotypes

对比环境和遗传对替代表型的控制

基本信息

批准号：
7512002
负责人：
JENNIFER A BRISSON
金额：
$ 9万
依托单位：
UNIVERSITY OF SOUTHERN CALIFORNIA
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-29 至 2009-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7512002
关键词：
Address Adult Aphids Apis Area Award Biological Biological Assay Biological Models Biology California Chromosome Mapping Complement Complex Computational Molecular Biology Condition Crowding Cues DNA Development Disease susceptibility Environment Exhibits Female Gas Chromatography Gene Expression Gene Expression Profile Genes Genetic Genetic Polymorphism Genetic Recombination Genetic Variation Genome Genomics Genotype Goals Height Individual Institution Laboratories Link Maps Mediating Mentors Metabolic Molecular Genetics Morphology Names Nature Pathway interactions Phase Phenotype Pisum sativum Population Positioning Attribute Predisposition Production Research Research Personnel Science Signal Transduction Staging Stimulus Study models System Systems Biology Training Universities Variant Weight Wing asexual base design developmental genetics insight male metabolomics programs response retinal rods transcriptomics

项目摘要

DESCRIPTION (provided by applicant): The proposed research addresses a fundamental question in biology: how do the environment and genotype interact to produce the phenotype? The pea aphid, an emerging genomic model system, exhibits dramatically different adult phenotypes of winged or unwinged morphs that are induced by environmental conditions in asexual females (a polyphenism) and by a single unidentified genetic locus in males (a polymorphism). Further, genetic variation for the female wing polyphenism segregates with the male wing polymorphism suggesting that the developmental networks underlying the polyphenism and polymorphism are not independent. I propose to (1) map and identify the male polymorphism gene to determine the developmental genetic basis of the male polymorphism, (2) characterize the transcriptional and metabolomic response to wing-inducing stimuli in the asexual females to determine the developmental genetic basis of the female polyphenism and (3) use a systems biology approach to link the results from (1) and (2) to provide a cohesive picture of how environmental signals and genetic signals interact to produce fundamentally similar adult alternative morphologies. The year-long mentored stage of this award focuses on developing a holistic view of the biological systems involved in a training environment that excels in systems and network biology: the laboratory of Dr. Sergey Nuzhdin in the Molecular and Computational Biology section at the University of Southern California. Following the mentored period, I will transition to a tenure-track position at an academic research institution. Relevance: All phenotypes, from our height and weight to our disease susceptibility, result from a complex interplay between our genes and our environment. This project aims to illuminate basic rules acting to integrate environmental and genetic signals in the production of the phenotype.

描述（由申请人提供）：拟议的研究解决了生物学中的一个基本问题：环境和基因型如何相互作用以产生表型？ PEA蚜虫是一种新兴的基因组模型系统，在无性女性的环境条件（一种多态性）和男性（多态性）中单个未识别的遗传基因座（一种多态性的遗传基因座）（一种多态性）表现出巨大不同的成年成人表型。此外，雌性翼多态性的遗传变异与男性翼多态性分离，表明多形和多态性的发育网络并非独立。 I propose to (1) map and identify the male polymorphism gene to determine the developmental genetic basis of the male polymorphism, (2) characterize the transcriptional and metabolomic response to wing-inducing stimuli in the asexual females to determine the developmental genetic basis of the female polyphenism and (3) use a systems biology approach to link the results from (1) and (2) to provide a cohesive picture of how environmental信号和遗传信号相互作用以产生基本相似的成人替代形态。为期一年的指导阶段，该奖项的重点是对培训环境中涉及的生物系统的整体观点，在系统和网络生物学方面擅长：Sergey Nuzhdin博士在南加州大学分子和计算生物学部分的实验室。在指导期之后，我将过渡到学术研究机构的终身任期。相关性：从我们的身高和体重到我们的疾病敏感性，所有表型都是由于我们的基因与环境之间的复杂相互作用所致。该项目旨在阐明作用的基本规则，以整合表型生产中的环境和遗传信号。