CORE D2: Confocal

CORE D2：共焦

• 批准号: 8318648
• 负责人: HUGO J BELLEN
• 金额: $ 17.17万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8318648
• 关键词: Cells; Complement; Computer software; Confocal Microscopy; Contract Services; Developmental Disabilities; Disease; Equipment; Fees; Genes; Hour; Human; Image; Individual; Intellectual functioning disability; Laboratories; Maintenance; Manufacturer Name; Mental Retardation and Developmental Disabilities Research Centers; Microscope; Pathologic; Performance; Potassium Channel; Proteins; Research; Research Personnel; Resolution; Services; Structure; System; Tissues; Training; Wages; Work; base; cost; cost effectiveness; experience; improved; meetings; member; molecular marker; mutant; protein distribution; protein structure function; quality assurance; repaired; synaptotagmin; syntaxin; vesicle-associated membrane protein

Confocal microscopy complements the work of most BCM-IDDRC investigators whose work entails analysis and description of protein distribution within cells or tissues. Among the specific strengths of this system are the capacity to use multiple markers concurrently and the ability to study the three-dimensional character of one or several related proteins with sub-cellular resolution. For members of the BCM-IDDRC, the three confocal systems have facilitated discovery and elucidation of genes involved in human disorders such as Rett and SCA1, functional analyses of genes including Mathi, Mecp2, Ataxin, Gfi-1, shar-pei, APP, VAP-33A, endophilin, crumbs, hrs, CSP, atonal, syntaxin, senseless, neurexin, ROP, discs lost, skittles, gutfeeling, synaptobrevin, synaptotagmin, apterous, Dpp and protein structure/function studies of potassium channel domains. These and similar studies have involved thirteen BCM-IDDRC laboratories on an ongoing basis and nine other BCM-IDDRC laboratories on an occasional basis. Cost Effectiveness: The original cost of the two Zeiss confocal microscope systems and related equipment was approximately $1.2 million. Operating costs per year including primarily service contracts, salary costs for user training and day to day maintenance and technical support of histological services are currrently approximately $128,000. Very few BCM-IDDRC laboratories could individually justify either the expense of purchasing and maintaining any one of the confocal systems or would fully utilize the full capacity of a single system. Quality Assurance: All of the microscopes in the Confocal core were purchase new, were under warranties during the first year and have since been continuously under service contracts with the manufacturers to assure all systems meet or exceed performance quality requirements. Preventative maintenance of the equipment includes regular cleaning of user inaccessible portions of the microscope systems and revision of microscope software as needed. Where feasible, we have in the past and will continue to improve the software and hardware associated with the microscopes to provide additional features, attain better equipment performance, and better meet new or changing user and regulatory requirements. The microscopes are observed on a daily basis and user serviceable portions of the systems are examined, aligned, cleaned and repaired on a regular basis by the core manager. Most of our users experience their initial use of Confocal microscopy using our equipment and training to obtain optimal images from the equipment is a significant component of quality. Each of our new users is provided with at least two hours of individual hands-on training with the core manager followed by required solo sessions and ongoing support as needed. F. UTILIZATION The confocal microscopy core will provide training, equipment and technical support to any BCM-IDDRC investigator who would benefit from use of the confocal microscope systems. We expect that BCM-IDDRC users will continue to benefit from the preferential access to the systems and because of the favorable fee structure in comparison with available alternatives

共聚焦显微镜补充了大多数BCM-IDDRC研究人员的工作，其工作需要分析 以及细胞或组织中蛋白质分布的描述。该系统的具体优势之一是 同时使用多个标记的能力以及研究的能力 具有亚细胞分辨率的一个或几个相关蛋白质。对于BCM-IDDRC的成员，三个 共聚焦系统有助于发现和阐明参与人类疾病（例如RETT）的基因 和SCA1，包括Mathi，Mecp2，ataxin，GFI-1，Shar-PEI，App，App，VAP-33A在内的基因的功能分析 内菲林，碎屑，人力资源，CSP，atonal，语法，无意义，神经毒素，rop，椎间盘丢失，spittles，gutfeeling，gutfeeling， 钾通道的Synaptobrevin，Synaptotagmin，Apterous，DPP和蛋白质结构/功能研究 域。这些和类似的研究涉及13个BCM-IDDRC实验室，并且 偶尔的其他九个BCM-IDDRC实验室。 成本效益： 两个蔡司共聚焦显微镜系统和相关设备的原始成本约为1.2美元 百万。每年运营成本，包括主要服务合同，用户培训的工资成本以及日期 组织学服务的一日维护和技术支持约为$ 128,000。非常 很少有BCM-IDDRC实验室可以单独证明购买和维护任何任何费用 共聚焦系统之一或将充分利用单个系统的全部容量。 质量保证： 共聚焦核心中的所有显微镜均购买了新的购买，第一年均受保修。 此后一直在与制造商签订的服务合同，以确保所有系统会议 或超出性能质量要求。设备的预防性维护包括常规 清洁显微镜系统的无法访问的部分以及将显微镜软件的修订为 需要。在可行的地方，我们有过去，并且将继续改善软件和硬件 与显微镜相关联，以提供其他功能，获得更好的设备性能以及 更好地满足新的或不断变化的用户和监管要求。每天都观察到显微镜 检查系统的基础和用户可维修部分，对定期进行对齐，对齐，清洁和维修 核心经理的基础。我们的大多数用户都会使用我们的最初使用共聚焦显微镜使用我们 设备和培训以从设备中获取最佳图像是质量的重要组成部分。 我们的每个新用户都至少与核心经理进行至少两个小时的个人动手培训 然后根据需要进行所需的个人会议和持续的支持。 F.利用率 共聚焦显微镜核心将为任何BCM-IDDRC提供培训，设备和技术支持 研究人员将受益于共聚焦显微镜系统的使用。我们希望BCM-IDDRC 用户将继续受益于对系统的优先访问，并且由于收费有利 与可用替代方案相比