Resource Core D - Bioinformatics and Statistical Analysis Core

资源核心 D - 生物信息学和统计分析核心

基本信息

批准号：
10676795
负责人：
Ernesto Guccione
金额：
$ 21.64万
依托单位：
ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-01 至 2026-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10676795
关键词：
ATAC-seq Acceleration Address Antisense Oligonucleotides Bioinformatics Biological Biology Biometry ChIP-seq Chromatin Collaborations Communication Communities Consultations Data Data Analyses Data Reporting Data Set Databases Development Disease Education Educational workshop Ensure Evolution Experimental Designs Fees Funding Future Genes Genomics Goals Hi-C Homeostasis Human Resources Image Individual Leadership Methodology Multiomic Data Multiplexed Ion Beam Imaging Output Proteomics RNA Splicing Randomized Reporting Reproducibility Research Research Design Research Methodology Research Personnel Research Project Grants Resources Sample Size Scholarship Services Skin Slide Stains Standardization Statistical Data Interpretation Statistical Models Technology Training Training and Education Visualization Work bioinformatics tool biological systems complex data computerized tools computing resources cost data integration data management data sharing data tools data visualization design diverse data exome sequencing experience experimental study improved innovation large datasets meetings novel prevent process repeatability single-cell RNA sequencing skin organogenesis statistics synergism tool transcriptomics web-based tool whole genome

项目摘要

SUMMARY – RESOURCE CORE D Recent advancements in the field of genomics, transcriptomics, proteomics and imaging have greatly enhanced our understanding of skin development, homeostasis and disease. Nonetheless access to state-of- the-art statistical and bioinformatic tools for the analysis and integration of these large datasets has a high barrier in terms of costs and expertise limiting reproducibility and innovation. The main objective of Core D, `Data Analysis and Integration' is to promote research in skin biology and disease by providing a central hub for statistical design, bioinformatic analysis and training across all projects. This will ensure a standardized and reproducible process to generate meaningful biological conclusions. Core D will provide a complete solution for experimental and statistical consultation, genomics, transcriptomics and immunohistochemical data analysis. It will provide data integration across all platforms and projects, data management, interpretation and reporting, through Skin-GLOW (Skin-Gene Level Omic Web Tool), a state-of-the-art uniformed and integrated database for data visualization and sharing. This interface will improve the reproducibility and validity of research findings and boost skin related discoveries. Core personnel will be embedded in skin research labs where they will become familiarized with skin as a biological system and the questions being asked; this will help ensure that they can most effectively advise lab personnel appropriate analyses of their data; conversely lab personnel will become more familiar with analysis tools. Core D leadership collectively have >30 years of experience in biostatistics and chromatin biology bioinformatic support. This strong team will work closely together to provide first-rate core service to SBDRC researchers. Core D will provide the following services to core users: (1) Coordinated and comprehensive management of computational resources and biostatistics and bioinformatics training at reduced cost to SBDRC users; (2) Statistical design and analysis services; (3) Standardized, state- of-the art bioinformatics expertise for genomics and transcriptomics-based approaches; (4) Standardized, state-of-the art bioinformatics expertise for spatial approaches, and (5) Develop pipelines and innovative tools for data integration across all platforms (i.e. Skin-GLOW). The core is highly innovative through its cutting-edge integrated bioinformatics analysis (e.g. spatial transcriptomics, sc-ATAC-seq and Iso-seq), which will enable SBDRC researchers to fully explore complex datasets, formulate novel hypotheses, as well as access to unique tools (i.e. Splice Switching Antisense Oligos -ASOs) for mechanistic studies and potential future therapies for skin related diseases. Significance: Collectively, Core D is constituted to provide state-of-the-art statistical and bioinformatics expertise, collaboration and training to all SBDRC investigators, while integrating multi-omic data in an interactive web-tool, to advance research in skin biology and disease. This in turn will facilitate discussion between investigators to increase the relevance, value and impact of their studies, and the development of additional bioinformatics tools tailored to the specific needs of the skin community.

摘要 – 资源核心 D 基因组学、转录组学、蛋白质组学和成像领域的最新进展极大地促进了增强了我们对皮肤发育、体内平衡和疾病的了解。用于分析和整合这些大型数据集的最先进的统计和生物信息学工具具有很高的成本和专业知识方面的障碍限制了可重复性和创新核心 D 的主要目标， “数据分析和整合”旨在通过提供一个中心枢纽来促进皮肤生物学和疾病的研究用于所有项目的统计设计、生物信息分析和培训，这将确保标准化和标准化。 Core D 将为产生有意义的生物学结论提供完整的解决方案。实验和统计咨询、基因组学、转录组学和免疫组织化学数据分析。将提供跨所有平台和项目的数据集成、数据管理、解释和报告，通过 Skin-GLOW（Skin-Gene Level Omic Web Tool），一个最先进的统一集成数据库该界面将提高研究结果的可重复性和有效性。并促进皮肤相关的发现。核心人员将被派往皮肤研究实验室。熟悉皮肤作为一个生物系统以及所提出的问题，这将有助于确保：他们可以最有效地建议实验室人员对其数据进行适当的分析；相反，实验室人员也会这样做；更加熟悉分析工具。Core D 领导层在这方面总共拥有超过 30 年的经验。这个强大的团队将紧密合作，提供生物统计学和染色质生物学的生物信息支持。为SBDRC研究人员提供一流的核心服务Core D将为核心用户提供以下服务：（1）计算资源与生物统计学和生物信息学的协调和综合管理为 SBDRC 用户提供降低成本的培训； (2) 统计设计和分析服务； (3) 标准化、国家化基于基因组学和转录组学方法的最先进的生物信息学专业知识；（4）标准化，最先进的空间方法生物信息学专业知识，以及 (5) 开发管道和创新工具用于跨所有平台的数据集成（即 Skin-GLOW）其核心通过其尖端技术具有高度创新性。综合生物信息学分析（例如空间转录组学、sc-ATAC-seq 和 Iso-seq），这将使 SBDRC 研究人员充分探索复杂的数据集，提出新颖的假设，并获取用于机制研究和潜在未来的独特工具（即剪接转换反义寡核苷酸 -ASO）意义：总的来说，Core D 旨在提供最先进的技术。为所有 SBDRC 研究人员提供统计和生物信息学专业知识、协作和培训，同时整合交互式网络工具中的多组学数据，以推进皮肤生物学和疾病的研究。促进研究者之间的讨论，以提高其研究的相关性、价值和影响力，以及开发适合皮肤界特定需求的其他生物信息学工具。