Imaging and Surgery Core

影像和手术核心

• 批准号: 10674672
• 负责人: Gwendalyn J Randolph
• 金额: $ 45.09万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10674672
• 关键词: Antibodies; Behavior; Biology; Blood Vessels; Blood flow; Brain region; Cells; Collaborations; Color; Coupled; Data; Data Analyses; Data Set; Diameter; Erythrocytes; Grant; High Density Lipoproteins; Image; Image Analysis; Image-Guided Surgery; Immune; Individual; Laboratories; Lipoproteins; Lymphatic; Macrophage; Meningeal; Meninges; Methods; Modality; Molecular; Natural Immunity; Operative Surgical Procedures; Oxygen; Pathogenesis; Pathology; Penetration; Procedures; Reagent; Refractory; Research; Research Personnel; Resolution; Role; Sampling; Science; Services; Speed; Stains; Standardization; Surgeon; Technology; Thinness; Three-Dimensional Imaging; Tissue imaging; Tissues; Tracer; Transplantation; combinatorial; cranium; experience; improved; indexing; innovation; intravital imaging; light microscopy; lymphatic vasculature; mouse model; neuroimmunology; novel; oxygen transport; photoacoustic imaging; programs; protocol development; skills; tissue fixing; tool; trafficking; two photon microscopy; two-photon

ABSTRACT – Core B The imaging and surgical core will provide state-of-the-art imaging services to the program. Dr. Randolph’s experience in organizing collaborative teams and her laboratory's background in macrophage biology and lymphatic/vascular trafficking of immune cells and lipoproteins will facilitate applications in imaging that will be tailored to the science of each project. The core will incorporate major contributions from highly expert co- investigators. Dr. Bernd Zinselmeyer is an expert in multiple modalities of light microscopy, including its application in studies of the CNS. He will contribute several lines of expertise in the grant, from confocal and 2- photon microscopy to high-speed epifluorescence imaging. Dr. Song Hu is an innovator in photoacoustic imaging with demonstrated expertise in using this technology to study the vasculature of the CNS. Dr. Igor Smirnov is an expert microsurgeon who will provide and oversee highly skilled surgical manipulations for the projects, including procedures like skull transplant and skull thinning. These co-investigators bring more than their state-of-the-art expertise; they also all bring demonstrated collaborations with one or more project leaders. By working together, this core team of investigators and affiliated staff will offer to all projects the following range of expert surgical procedures and imaging applications. The aims of the imaging and surgical core are to (1) carry out deep-tissue imaging of meninges and outer regions of the brain, (2) apply combinatorial methods (2-photon, high-speed widefield, and photoacoustic imaging) for intravital imaging to investigate macrophage behavior and blood flow dynamics in the meninges, and (3) employ established and novel tools to quantify molecular outflow from the meningeal space.

摘要 – 核心 B 成像和手术核心将为伦道夫博士的项目提供最先进的成像服务。 组织协作团队的经验以及她的实验室在巨噬细胞生物学方面的背景 免疫细胞和脂蛋白的淋巴/血管运输将促进成像应用 根据每个项目的科学特点量身定制的核心将包含高度专家合作的主要贡献。 Bernd Zinselmeyer 博士是多种光学显微镜技术的专家，包括光学显微镜技术。 他将在资助中贡献多方面的专业知识，包括共聚焦和 2- 胡松博士是光声成像领域的创新者。 Igor Smirnov 博士在使用该技术研究中枢神经系统脉管系统方面拥有丰富的专业知识。 专家显微外科医生将为项目提供和监督高技能的手术操作，包括 这些合作研究人员带来的不仅仅是他们最先进的技术。 专业知识；他们还与一名或多名项目负责人进行了合作， 这个由研究人员和附属人员组成的核心团队将为所有项目提供以下范围的专家外科手术 成像和手术核心的目的是（1）进行深层组织。 脑膜和大脑外部区域的成像，(2) 应用组合方法（2 光子、高速 宽场和光声成像）用于活体成像以研究巨噬细胞行为和血流 脑膜中的动力学，以及（3）采用现有的和新颖的工具来量化脑膜中的分子流出 脑膜空间。