Intercellular Signaling in Myxococcus xanthus

黄色粘球菌的细胞间信号转导

• 批准号: 7813783
• 负责人: Wenyuan Shi
• 金额: $ 34.04万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7813783
• 关键词: Adherence; Amino Acids; Antibodies; Bacteria; Behavior; Binding; Biochemical; Biochemistry; Bioinformatics; Biological; Biological Models; Candidate Disease Gene; Cell membrane; Cell physiology; Cell surface; Cells; Chemicals; Chemotaxis; Complex; Cytoplasm; Data; Development; Event; Flagella; Frequencies; Fruit; Future; Gene Expression; Genes; Genetic; Genome; Grant; Homologous Gene; Ion Channel; Knowledge; Label; Lead; Lectin; Life Cycle Stages; Location; Measures; Mechanics; Membrane; Methylation; Microbial Biofilms; Microscopic; Modeling; Modification; Molecular; Molecular Analysis; Monitor; Morphogenesis; Motion; Motor; Mutagenesis; Mutate; Myxococcus xanthus; Nature; Operon; Pathogenesis; Pattern; Phage Attachment Site; Phenotype; Physiological; Physiology; Pilum; Plasmids; Post-Translational Protein Processing; Posttranslational Amino Acid Modification; Production; Property; Proteins; Proteomics; Reagent; Regulation; Reporter; Research Personnel; Resistance; Role; Signal Transduction; Site; Social Behavior; Solid; Spatial Distribution; Surface; Swimming; System; Techniques; Testing; Time; Tissues; Touch sensation; Transcriptional Regulation; Virulent; base; cell motility; combat; epsin; extracellular; fascinate; follow-up; genetic analysis; genetic regulatory protein; improved; insight; microbial; mutant; neuronal cell body; pathogenic bacteria; research study; scaffold; sensor; sensory system; social; tool

DESCRIPTION (provided by applicant): Intercellular signaling is essential for complex microbial events such as development, pathogenesis and biofilm formation. Myxococcus xanthus is an excellent model bacterium for studying intercellular signaling because of its complex life cycle and coordinated social behaviors. This study focuses on M. xanthus social gliding motility and chemotaxis, a multi-cellular function involved in vegetative swarming and fruiting body formation. Previous studies show that social gliding motility/chemotaxis of M. xanthus involves the complex interactions of three major cellular components: type IV pili (TFP), exopolysacchrides (EPS), and a chemotaxis-like Frz sensory system (Frz). This study aims to understand the genetic and biochemical basis of M. xanthus social gliding motility/chemotaxis. Three specific aims are proposed in this application. Aim 1 is to analyze the genetic and biochemical properties of PilA to further understand the role of TFP in S-motility. Aim 2 is to study the molecular mechanisms associated with temporal and spatial control of EPS production and distribution to further understand the role of EPS in S-motility. Aim 3 is to explore the interaction between TFP & EPS and its associated biological events. The successful execution of these proposed studies will provide a molecular understanding of the TFP-EPS-Frz-associated social gliding motility and chemotaxis. The findings will reveal insightful biological knowledge about intercellular signaling events during the complex development of M. xanthus. Furthermore, these studies could discover the possible evolutionary connections between flagella based swimming motility and TFP dependent gliding motility. Since TFP, EPS and chemosensory proteins are also the key virulent factors for a variety of pathogenic bacteria during biofilm formation and tissue adherence and invasion, the knowledge gained from this study could also lead to new ways to combat microbial pathogenesis.

描述（由申请人提供）：细胞间信号传导对于复杂的微生物事件（例如发育、发病机制和生物膜形成）至关重要。黄色粘球菌因其复杂的生命周期和协调的社会行为而成为研究细胞间信号传导的优秀模型细菌。本研究重点关注 M. xanthus 的社会滑行运动和趋化性，这是一种参与营养集群和子实体形成的多细胞功能。先前的研究表明，M. xanthus 的社会滑翔运动/趋化性涉及三种主要细胞成分的复杂相互作用：IV 型菌毛 (TFP)、胞外多糖 (EPS) 和类趋化性 Frz 感觉系统 (Frz)。本研究旨在了解 M. xanthus 社会滑翔运动/趋化性的遗传和生化基础。本申请提出了三个具体目标。目标 1 是分析 PilA 的遗传和生化特性，以进一步了解 TFP 在 S 运动中的作用。目标 2 是研究 EPS 产生和分布的时间和空间控制相关的分子机制，以进一步了解 EPS 在 S 运动中的作用。目标 3 是探索 TFP 和 EPS 之间的相互作用及其相关的生物事件。这些拟议研究的成功执行将为 TFP-EPS-Frz 相关的社会滑翔运动和趋化性提供分子理解。这些发现将揭示有关 M. xanthus 复杂发育过程中细胞间信号传导事件的深刻生物学知识。此外，这些研究可以发现基于鞭毛的游泳运动和依赖 TFP 的滑行运动之间可能的进化联系。由于TFP、EPS和化学感应蛋白也是多种病原菌在生物膜形成、组织粘附和侵袭过程中的关键毒力因子，因此从这项研究中获得的知识也可能带来对抗微生物发病机制的新方法。

项目成果

Chemotaxis in pathogenic spirochetes: directed movement toward targeting tissues?

• 发表时间: 2000-10
• 期刊: Journal of molecular microbiology and biotechnology
• 影响因子: 1.2
• 作者: R. Lux;A. Moter;W. Shi

Killing of Escherichia coli by Myxococcus xanthus in aqueous environments requires exopolysaccharide-dependent physical contact.

• DOI: 10.1007/s00248-013-0252-x
• 发表时间: 2013-10
• 期刊: MICROBIAL ECOLOGY
• 影响因子: 3.6
• 作者: Pan, Hongwei;He, Xuesong;Lux, Renate;Luan, Jia;Shi, Wenyuan
• 通讯作者: Shi, Wenyuan

Three-dimensional macromolecular organization of cryofixed Myxococcus xanthus biofilms as revealed by electron microscopic tomography.

电子显微镜断层扫描显示冷冻固定黄色粘球菌生物膜的三维大分子组织。

• DOI: 10.1128/jb.01333-08
• 发表时间: 2009
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Palsdottir,Hildur;Remis,JonathanP;Schaudinn,Christoph;O'Toole,Eileen;Lux,Renate;Shi,Wenyuan;McDonald,KentL;Costerton,JWilliam;Auer,Manfred
• 通讯作者: Auer,Manfred

Analysing protein-protein interactions of the Myxococcus xanthus Dif signalling pathway using the yeast two-hybrid system.

• DOI: 10.1099/mic.0.27743-0
• 发表时间: 2005-05
• 期刊: Microbiology
• 影响因子: 1.5
• 作者: H. Lancero;Schryl Castaneda;N. Caberoy;Xiaoyuan Ma;A. Garza;W. Shi

Isolation and characterization of a suppressor mutation that restores Myxococcus xanthus exopolysaccharide production.

• DOI: 10.1099/mic.0.031070-0
• 发表时间: 2009-11
• 期刊: Microbiology (Reading, England)
• 作者: Black WP;Xu Q;Cadieux CL;Suh SJ;Shi W;Yang Z
• 通讯作者: Yang Z