INTERCELLULAR SIGNALING IN MYXOCOCCUS XANTHUS

黄色粘球菌的细胞间信号传导

• 批准号: 6181282
• 负责人: Wenyuan Shi
• 金额: $ 23.14万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6181282
• 关键词: Myxococcus; bacterial genetics; biological signal transduction; cell cell interaction; chemoattractants; chemotaxis; extracellular matrix; gene expression; gene mutation; microorganism growth; molecular cloning; nucleic acid sequence

DESCRIPTION: The long-term goal of this research is to understand the molecular mechanisms which bacteria use for cell-cell interactions. This proposal focuses on studying intercellular signaling of a model bacterium, Myxococcus xanthus. M. xanthus is a Gram-negative bacterium that exhibits a variety of multicellular behaviors. When deprived of nutrients, cells aggregate to form fruiting bodies, a process that involves chemotactic movement and intercellular signaling. Previous studies showed that developmental cells release chemoattractants during starvation and that the chemoattractants are sensed by chemosensory systems and relayed into the frz chemotaxis system. The objectives of the study are to find out the chemical natures of the developmental chemoattractants and to understand how the signal molecules are sensed by the chemosensory systems and relayed into the frz chemotaxis system. Described here are the genetic, molecular biological and biochemical methods to be used: 1) A set of mutants which fail to produce or sense the developmental chemoattractants will be isolated and characterized. 2) The interesting mutation genes will be cloned and sequenced; the regulation of gene expression will be studied. 3) The biochemical properties of the gene products will be studied with emphasis on the functions for producing or sensing developmental chemoattractants; extracellular matrix of developmental cells will be collected and fractionated for the developmental chemoattractants. Knowledge of intercellular signaling of M. xanthus could give insights into signaling mechanisms of other biological systems.

描述：这项研究的长期目标是了解 细菌用于细胞间相互作用的分子机制。 这 该提案的重点是研究模型细菌的细胞间信号传导， 黄色粘球菌。 M. xanthus 是一种革兰氏阴性细菌，表现出 多种多细胞行为。 当细胞缺乏营养时 聚集形成子实体，这是一个涉及趋化的过程 运动和细胞间信号传导。 先前的研究表明 发育细胞在饥饿期间释放化学引诱剂，并且 化学引诱剂被化学感应系统感知并传递到 frz 趋化系统。 该研究的目的是找出化学物质 发育化学引诱剂的性质并了解 信号分子被化学感应系统感知并传递到 frz趋化系统。 这里描述的是遗传、分子生物学和生化方法 使用：1）一组不能产生或感知 发育化学引诱剂将被分离和表征。 2) 的 有趣的突变基因将被克隆并测序；的监管 将研究基因表达。 3）基因的生化特性 将重点研究产品的生产或功能功能 感知发育化学引诱剂；细胞外基质 将收集并分离发育细胞以用于发育 化学引诱剂。 了解 M. xanthus 的细胞间信号传导可以 深入了解其他生物系统的信号传导机制。