Prevalence and Clinical Correlates of Thirdhand Smoke Exposure in a Pediatric Patient Population

儿科患者群体中三手烟暴露的患病率和临床相关性

基本信息

批准号：
10673044
负责人：
E. Melinda Mahabee-Gittens
金额：
$ 52.3万
依托单位：
CINCINNATI CHILDRENS HOSP MED CTR
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-08-15 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10673044
关键词：
4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone 8-hydroxy-2&apos -deoxyguanosine Accident and Emergency department Accidents Affect Age Air Animals Asthma Biochemical Biological Biological Markers Carpet Chemicals Child Child Care Childhood Cigarette Clinical Cotinine DNA DNA Damage Disease Dust Environment Exposure to Foundations Frequencies Furniture Gases Hand Health Health Care Visit Home Home visitation Hospitalization Housing Human Impairment In Vitro Inflammation Inflammatory Isoprostanes Laboratories Life Link Lipids Longitudinal cohort study Mus Nicotine Nitrosamines Oxidants Oxidative Stress Oxides Parents Pattern Phase Pollution Prevalence Proteins Reaction Reporting Research Respiratory Signs and Symptoms Respiratory physiology Sampling Smoke Smoker Smoking Source Surface TNF gene Techniques Time Tobacco Tobacco Smoke Pollution Tobacco smoke Tobacco use Toddler Toy Validation carcinogenicity clinical effect clinical risk cytokine demographics design environmental tobacco smoke environmental tobacco smoke exposure innovation insight non-smoker oxidation patient population pediatric emergency pediatric patients pollutant prospective pulmonary function repository respiratory response response biomarker screening thirdhand smoke tobacco smoke exposure urinary

项目摘要

PROJECT SUMMARY Thirdhand smoke (THS) is the persistent toxic residue of tobacco smoke pollutants that attaches to surfaces, remains in dust, and becomes embedded in environments where secondhand smoke (SHS) has been released into the air. Even when parents are diligent in attempting to shield their children from tobacco smoke, exposure to SHS (SHSe) and THS (THSe) still occurs. Residue from previously smoked tobacco at home or in a car creates a pervasive reservoir of tobacco smoke pollutants that are within reach of a child's hand such as on carpets, furniture, clothes, and toys, and breathed in dust. Homes of smokers become reservoirs of these pollutants that persist for years, become more toxic with age, and are later reemitted into the gas phase, or react with common ambient oxidants and other environmental compounds to yield secondary pollutants such as carcinogenic tobacco-specific nitrosamines. The clinical effects of THSe are unknown because prior research has examined children exposed to both SHS and THS. Studies exclusively focusing on THSe are needed. Thus, we have conducted in-vitro, animal, and pilot pediatric studies that demonstrate that THSe is associated with high levels of inflammatory cytokines, respiratory illnesses, and oxidative stress including DNA damage, in mice. We have found even in the presence of smoking bans, children still have high levels of THS pollution on their hands and that nonsmokers' homes have high THS pollution levels. However, in the absence of large-scale, rigorous human studies, conclusions about the clinical effects of THSe cannot be made. Thus, we propose an innovative prospective longitudinal cohort study that will move the field forward and away from the laboratory to a pediatric clinical setting as the first pediatric study to use biochemical validation to identify children exposed to THS-only over time. We will screen 1000 children from the pediatric emergency department, a setting which cares for children with varying tobacco smoke exposure (TSE) levels and illnesses. Using strict screening and chemical analysis techniques, we will identify and compare children that comprise three distinct TSE groups: 1) THSe only group: live with nonsmokers, no reported SHSe, confirmed negative/low levels of SHSe, elevated levels of THSe; 2) Mixed SHSe+THSe group: live with smokers, reported SHSe, confirmed moderate/high levels of SHSe, high levels of THSe; and 3) No TSE group: live with nonsmokers, no reported SHSe, confirmed negative levels of SHSe and THSe. The prevalence and levels of THSe, demographics, and exposure and illness patterns of these groups will be compared over time. Next, we will disentangle which environmental sources contribute to THSe levels and the association between THSe and inflammatory (i.e., cytokines) and oxidative (i.e., DNA, lipid, protein oxidation) biomarkers and TSE- related clinical illnesses. Results will provide unique insights into how THSe and sources of tobacco smoke pollution affect the inflammatory, oxidative, and clinical responses in children. The potential impact of these findings is substantial as currently, the level of clinical risk attributable to THSe is unknown.

项目概要三手烟 (THS) 是烟草烟雾污染物的持久有毒残留物，附着在表面，残留在灰尘中，并嵌入二手烟 (SHS) 存在的环境中被释放到空气中。即使父母努力保护孩子免受烟草侵害烟雾，暴露于 SHS (SHSe) 和 THS (THSe) 仍然会发生。以前吸过的烟草残留物家中或汽车内会造成烟草烟雾污染物的普遍储存，而这些污染物都在儿童的接触范围内。手接触地毯、家具、衣服和玩具等，并吸入灰尘。吸烟者的家变得这些污染物的储存库会持续多年，随着时间的推移，毒性会变得更大，随后会重新排放到气相，或与常见的环境氧化剂和其他环境化合物反应，产生二次污染物，例如致癌的烟草特有的亚硝胺。 THSe 的临床效果尚不清楚因为之前的研究已经对接触过二手烟和二手烟的儿童进行了调查。研究专门集中于需要这些。因此，我们进行了体外、动物和试点儿科研究，证明 THSe 与高水平的炎症细胞因子、呼吸道疾病和氧化应激有关包括小鼠体内的 DNA 损伤。我们发现，即使存在禁烟令，儿童的吸烟率仍然很高他们手上的 THS 污染水平以及非吸烟者家中的 THS 污染水平很高。然而，在缺乏大规模、严格的人体研究的情况下，无法得出有关 THSe 临床效果的结论制成。因此，我们提出了一项创新的前瞻性纵向队列研究，该研究将推动该领域向前发展并从实验室转移到儿科临床环境，这是第一个使用生化的儿科研究验证以确定随着时间的推移仅接触 THS 的儿童。我们将从儿科筛查1000名儿童急诊科，一个照顾不同烟草烟雾暴露 (TSE) 水平的儿童的机构和疾病。使用严格的筛选和化学分析技术，我们将识别和比较儿童包括三个不同的 TSE 组：1) 仅 THSe 组：与不吸烟者住在一起，没有报告 SHSe，确认 SHSe 水平为负/低水平，THSe 水平升高； 2) 混合 SHSe+THSe 组：一起生活据报道，吸烟者的 SHSe 水平中等/高，THSe 水平高； 3) 无 TSE 组：与不吸烟者住在一起，没有报告 SHSe，确认 SHSe 和 THSe 水平为阴性。患病率和随着时间的推移，这些群体的 THSe 水平、人口统计、暴露和疾病模式将进行比较。接下来，我们将阐明哪些环境来源会影响 THSe 水平以及两者之间的关联 THSe 和炎症（即细胞因子）和氧化（即 DNA、脂质、蛋白质氧化）生物标志物和 TSE- 相关临床疾病。结果将为 THSe 和烟草烟雾来源提供独特的见解污染影响儿童的炎症、氧化和临床反应。这些的潜在影响目前的研究结果很重要，THSe 引起的临床风险水平尚不清楚。

项目成果

期刊论文数量（2）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Smoking Cessation Level of Contemplation in the Pediatric Emergency Department.

儿科急诊室的戒烟水平。

DOI：
发表时间：
2018
期刊：
影响因子：
0
作者：
Michelle, K Arzubi Hughes;Melinda, Mahabee;Denise, Dowd M;Joan, Giovanni
通讯作者：
Joan, Giovanni

Neonatal AVPR1a Methylation and In-Utero Exposure to Maternal Smoking.

新生儿 AVPR1a 甲基化和子宫内母亲吸烟暴露。

DOI：
发表时间：
2023-10-13
期刊：
影响因子：
4.6
作者：
Nidey, Nichole;Bowers, Katherine;Ding, Lili;Ji, Hong;Ammerman, Robert T;Yolton, Kimberly;Mahabee;Folger, Alonzo T
通讯作者：
Folger, Alonzo T