SPIN TRAPPING FREE RADICAL IN BIOLOGY AND MEDICINE

生物学和医学中的自旋捕获自由基

• 批准号: 3104294
• 负责人: EDWARD G JANZEN
• 金额: $ 36.07万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-10 至 1996-01-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3104294
• 关键词: analytical chemistry; biomedical equipment resource; electron spin resonance spectroscopy; free radicals; mass spectrometry

This proposal is submitted in response to a need for establishing a resource center in spin trapping for biomedical research. The first step is to apply for a resource-related research project grant within the Biomedical Research Technology Program. Funds are requested for the development of the resource technology, namely spin trapping, over a period of 5 years with the expectation that an application for funds to establish a National Center in this resource technology will be submitted at the end of this period. Specifically, the objective of this proposal is to develop improved methods for the detection, identification and quantitation of free radicals in biological systems with special emphasis on spin trapping techniques involving both ESR and Mass Spectroscopy. New spin traps will be synthesized and new methods of analyzing spin trapping products will be developed. Methods for quantitating spin trapping events will be developed. An extensive hyperfine splitting constant data base will be created and completed for each commonly used spin trap. Access to this data base and/or available information from in-house personnel will be established through electronic communication links. Collaborators will have the immediate benefit of results obtained in this project.

该建议是为了建立一个需要 用于生物医学研究的自旋陷阱的资源中心。 第一步 是申请与资源相关的研究项目赠款 生物医学研究技术计划。 要求资金 资源技术的开发，即旋转陷阱，一段时间 5年的期望是建立资金的申请 该资源技术中的国家中心将在最后提交 这个时期。 具体而言，该提议的目的是发展 改进了自由检测，识别和定量的方法 生物系统中的激进分子特别强调自旋陷阱 涉及ESR和质谱的技术。 新的自旋陷阱将 合成，分析旋转诱捕产品的新方法将是 发达。 定量自旋陷阱事件的方法将是 发达。 广泛的超精细分裂恒定数据库将是 为每个常用的自旋陷阱创建并完成。 访问这个 来自内部人员的数据库和/或可用信息将是 通过电子通信链接建立。 合作者将 在该项目中获得的结果有直接的好处。