National Biomedical Resource for Electron-Spin Resonance Spectroscopy (ACERT)

国家电子自旋共振光谱生物医学资源 (ACERT)

• 批准号: 10430665
• 负责人: BRIAN R CRANE
• 金额: $ 133.64万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10430665
• 关键词: 2019-nCoV; Acquired Immunodeficiency Syndrome; Address; Advertisements; Alzheimer' s Disease; Bacterial Infections; Biomedical Research; Collaborations; Collection; Communities; Complex; Computational Technique; Computer Analysis; Computer software; Custom; Data; Data Analyses; Data Collection; Depressive disorder; Development; Disease; Ebola virus; Education and Outreach; Educational workshop; Electron Nuclear Double Resonance; Electron Spin Resonance Spectroscopy; Equipment; Eye diseases; Frequencies; Funding; Future; Grant; Heart Diseases; Home; Human Resources; Hypersensitivity; Infertility; Inflammation; International; Journals; Label; Laboratories; Leadership; Learning; Malignant Neoplasms; Methodology; Methods; Mission; Modernization; Molecular; Molecular Biology; Molecular Structure; Monoclonal Antibody R24; Motion; Newsletter; Noise; Paper; Parkinson Disease; Physiologic pulse; Postdoctoral Fellow; Procedures; Process; Protein Dynamics; Proteins; Publishing; RNA; Recovery; Reporting; Research Personnel; Resources; Role; Running; Sampling; Sampling Studies; Schedule; Scheme; Schizophrenia; Scientist; Seasons; Series; Services; Signal Transduction; Site; Societies; Special Equipment; Strategic Planning; Structure; Students; System; Techniques; Technology; Temperature; Tertiary Protein Structure; Time; Training; United States National Institutes of Health; Virus Diseases; Visit; analysis pipeline; base; biological systems; biomedical resource; computerized data processing; computing resources; experience; experimental study; frontier; innovation; instrument; instrumentation; lectures; macromolecule; meetings; molecular dynamics; nanosecond; nervous system disorder; novel; online resource; operation; outreach; programs; protein complex; protein structure; signal processing; success; virtual; 2019-nCoV; Acquired Immunodeficiency Syndrome; Address; Advertisements; Alzheimer' s Disease; Bacterial Infections; Biomedical Research; Collaborations; Collection; Communities; Complex; Computational Technique; Computer Analysis; Computer software; Custom; Data; Data Analyses; Data Collection; Depressive disorder; Development; Disease; Ebola virus; Education and Outreach; Educational workshop; Electron Nuclear Double Resonance; Electron Spin Resonance Spectroscopy; Equipment; Eye diseases; Frequencies; Funding; Future; Grant; Heart Diseases; Home; Human Resources; Hypersensitivity; Infertility; Inflammation; International; Journals; Label; Laboratories; Leadership; Learning; Malignant Neoplasms; Methodology; Methods; Mission; Modernization; Molecular; Molecular Biology; Molecular Structure; Monoclonal Antibody R24; Motion; Newsletter; Noise; Paper; Parkinson Disease; Physiologic pulse; Postdoctoral Fellow; Procedures; Process; Protein Dynamics; Proteins; Publishing; RNA; Recovery; Reporting; Research Personnel; Resources; Role; Running; Sampling; Sampling Studies; Schedule; Scheme; Schizophrenia; Scientist; Seasons; Series; Services; Signal Transduction; Site; Societies; Special Equipment; Strategic Planning; Structure; Students; System; Techniques; Technology; Temperature; Tertiary Protein Structure; Time; Training; United States National Institutes of Health; Virus Diseases; Visit; analysis pipeline; base; biological systems; biomedical resource; computerized data processing; computing resources; experience; experimental study; frontier; innovation; instrument; instrumentation; lectures; macromolecule; meetings; molecular dynamics; nanosecond; nervous system disorder; novel; online resource; operation; outreach; programs; protein complex; protein structure; signal processing; success; virtual

This is an R24 proposal to establish a Regional and National Resource in modern Electron-Spin Resonance (ESR) Technology for use by the biomedical community. This resource will build upon 20 years of a very successful National Biomedical Center: ACERT (funded by an NIH P41 grant), in which the new ESR technologies that were developed, driven by biomedical collaborators, were then provided to extensive collaborators and service projects. In its new configuration as an R24 resource, it will focus on user services and collaborations utilizing the already established extensive and unique technologies that have already been developed. We have a well- established administration and operations scheme for this objective. Through outreach efforts we plan to significantly expand the user base both nationally and internationally. We will be providing extensive educational and training capabilities for the expanded user base. This will include a series of workshops, on-line resources, in house training and visits to other labs. The outreach will include tutorial papers in key journals, virtual meetings with the International ESR Society, video lectures on YouTube, and advertisements in leading Journals and Newsletters. The ESR resources we provide are in many ways unique in the world. In addition to our commercial spectrometers for cw and pulse ESR covering the frequency range of 9 to 35 GHz, we have extensive and unique custom-home built spectrometers covering the range of frequencies 9 to 240 GHz. These spectrometers are dedicated to a full range of ESR methods utilized by the biomedical community, especially including techniques for conducting pulse dipolar ESR (PDS), which provides distances between labeled protein and RNA sites from which protein tertiary structure may be inferred. It is applicable to protein complexes in their natural states. ACERT has developed methods that both significantly increase the sensitivity to low sub- micromolar protein concentrations and very small samples, and they greatly increase the throughput, enabling a greater rate of processing of the many samples studied in PDS. While PDS supplies protein structures, the additional techniques of multi-frequency ESR and pulsed 2D-ESR at ambient temperatures supply details of protein dynamics, important in learning about structure/function; this includes the unique ability to study in real time nanosecond to microsecond dynamics, which will address such issues as domain motions in proteins. Our innovative software enables the fitting of the complex 1- and 2-D spectra to extract the details of the complex protein motions, and our recent developments of signal processing software have led to unique packages for greatly increasing the signal-to-noise of weak ESR signals while providing great fidelity, as well as accurately transforming the results of PDS experiments into (complex) distance distributions. These software methods will also enhance the throughput of the Resource.

This is an R24 proposal to establish a Regional and National Resource in modern Electron-Spin Resonance (ESR) 生物医学界使用的技术。该资源将建立在20年的非常成功的基础上 National Biomedical Center: ACERT (funded by an NIH P41 grant), in which the new ESR technologies that were 然后由生物医学合作者驱动，然后向广泛的合作者和服务提供 项目。在其新的配置作为R24资源中，它将专注于使用用户服务和协作 已经开发的已经建立的广泛而独特的技术。我们有一个很好的 为此目标建立的管理和运营计划。通过宣传努力，我们计划 在国内和国际上显着扩大用户群。我们将提供广泛的教育 以及扩展的用户群的培训功能。这将包括一系列研讨会，在线资源， in house training and visits to other labs.外展将包括关键期刊中的教程论文，虚拟 与国际ESR协会的会议，YouTube上的视频演讲以及领先期刊的广告 和新闻通讯。我们提供的ESR资源在世界上在许多方面都是独一无二的。除了我们 CW和脉冲ESR的商业光谱仪覆盖9至35 GHz的频率范围，我们有广泛的 以及涵盖9至240 GHz的频率范围的独特定制式建筑光谱仪。这些 光谱仪专用于生物医学界使用的全部ESR方法，尤其是 包括用于传导脉冲偶极ESR（PDS）的技术，该技术在标记蛋白之间提供距离 以及可以推断蛋白质三级结构的RNA位点。它适用于蛋白质复合物 their natural states. Acert开发了方法，这些方法既显着提高了对低亚的敏感性 微摩尔蛋白浓度和非常小的样品，它们大大增加了吞吐量，使得 a greater rate of processing of the many samples studied in PDS. PDS提供蛋白质结构，而 在环境温度下，多频ESR和脉冲2D-ESR的其他技术供应细节 蛋白质动力学，对于学习结构/功能很重要；这包括学习的独特能力 实时纳秒至微秒动力学，该动力学将解决诸如蛋白质中的域运动等问题。 我们的创新软件使复杂的1-和2-D光谱的拟合能够提取复合物的细节 蛋白质运动以及我们最近对信号处理软件的发展已导致独特的包裹 大大增加了弱ESR信号的信号，同时提供了极大的保真度 将PDS实验的结果转换为（复杂）距离分布。这些软件方法将 还可以增强资源的吞吐量。