Adaptive Optics Instrumentation for Advanced Ophthalmic Imaging

用于高级眼科成像的自适应光学仪器

基本信息

批准号：
7931934
负责人：
DAVID R WILLIAMS
金额：
$ 101.25万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-03-01 至 2013-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7931934
关键词：
Affect Age related macular degeneration Axon Biological Markers Biomedical Engineering Blood capillaries Burn injury Cells Clinic Clinical Research Clinical Trials Computer software Computers and Advanced Instrumentation Dendrites Detection Development Devices Disease Disease Progression Early Diagnosis Engineering Environment Eye Eye Movements Fluorescence Fluorescent Dyes Fluorescent Probes Functional disorder Funding Future Generations Genetic Markers Genotype Glaucoma Grant Human Hybrids Image Imagery Indiana Individual Inherited International Label Laboratories Lasers Lead Leukocytes Life Light Macular degeneration Measures Methods Microscopic Monkeys Montana Motion Mus Ophthalmoscopes Ophthalmoscopy Optics Patients Phase Phenotype Photoreceptors Principal Investigator Rattus Research Research Personnel Resolution Retina Retinal Retinal Cone Retinal Degeneration Retinal Diseases Rodent Scanning Signal Transduction Structure System Techniques Technology Time Treatment Efficacy Universities adaptive optics austin capillary cellular imaging coping design disorder of macula of retina ergonomics fluorescence imaging ganglion cell image processing in vivo inherited retinal degeneration innovation instrument neuronal cell body next generation physical science public health relevance relating to nervous system response skills software development tool vision science

项目摘要

DESCRIPTION (provided by applicant): This Bioengineering Research Partnership is a consortium of 6 laboratories that are building adaptive optics scanning laser ophthalmoscopes (AOSLOs) and applying them to microscopic examination of the living normal and diseased retina. The principal investigator is David Williams (University of Rochester) who introduced the first successful adaptive optics instruments to vision science. Other lead investigators include: Steve Burns (Indiana University) an international leader in laser scanning ophthalmoscopy, John Flannery (UC, Berkeley) an expert in retinal degeneration and the development of retinal biomarkers, and Austin Roorda (UC, Berkeley) who designed the first adaptive optics scanning laser ophthalmoscopes, David Arathorn (Montana State University) who brings strong mathematical skills and software development tools for tracking the eye in AOSLOs, and R. Daniel Ferguson (Physical Sciences, Inc.) whose expertise is in the optical engineering of innovative eye tracking systems. During years 1-5 of the previous funding period, the partnership designed and built four AOSLO instruments and two more instruments are under construction. These devices have produced the first images ever of numerous microscopic structures in the living eye including the RPE cell mosaic, single leucocytes flowing in the smallest retinal capillaries, and fluorescently-labelled ganglion cell dendrites, axons and cell bodies. In addition, technical challenges for imaging eyes ranging in size from human to rodent have been overcome. The partnership is now proposing continued funding for years 6-10 to develop new capabilities for these instruments such as a combined hardware and software approach to reduce the effects of eye motion on high resolution retinal imagery. We also will develop a new generation of instruments with special capabilities, such as the ability to image ganglion cells in the living human eye without the use of fluorescent dyes, and the ability to optically record neural responses from specific retinal cells. PUBLIC HEALTH RELEVANCE: This application will develop a technology, adaptive optics scanning laser ophthalmoscopy, for taking extremely sharp pictures of the inside of the living eye, so sharp that individual cells can be seen. This technology will be used to study diseases such as age-related macular degeneration and glaucoma. It may allow the earlier detection of retinal disease, better tracking of disease progression, and the efficacy of therapies for retinal disease.

描述（由申请人提供）：该生物工程研究合作伙伴关系是一个由 6 个实验室组成的联盟，正在建造自适应光学扫描激光检眼镜 (AOSLO) 并将其应用于活体正常和患病视网膜的显微镜检查。首席研究员是大卫·威廉姆斯（罗切斯特大学），他将第一个成功的自适应光学仪器引入视觉科学。其他主要研究人员包括：史蒂夫·伯恩斯（印第安纳大学），激光扫描检眼镜领域的国际领导者；约翰·弗兰纳里（加州大学伯克利分校），视网膜变性和视网膜生物标记物开发方面的专家；以及奥斯汀·鲁达（加州大学伯克利分校），他设计了第一个自适应光学扫描激光检眼镜，David Arathorn（蒙大拿州立大学）带来了强大的数学技能和软件开发工具，用于在 AOSLO 中跟踪眼睛，R. Daniel Ferguson（物理科学公司）的专业知识是创新眼动追踪系统的光学工程。在上一个资助期的第 1-5 年，该合作伙伴设计并建造了四台 AOSLO 仪器，另外两台仪器正在建造中。这些设备首次生成了活体眼睛中众多微观结构的图像，包括 RPE 细胞镶嵌体、在最小的视网膜毛细血管中流动的单个白细胞，以及荧光标记的神经节细胞树突、轴突和细胞体。此外，对从人类到啮齿动物的眼睛进行成像的技术挑战也已被克服。该合作伙伴关系现提议在 6-10 年继续提供资金，为这些仪器开发新功能，例如结合硬件和软件方法来减少眼球运动对高分辨率视网膜图像的影响。我们还将开发具有特殊功能的新一代仪器，例如能够在不使用荧光染料的情况下对活体人眼中的神经节细胞进行成像，以及光学记录特定视网膜细胞的神经反应的能力。公共健康相关性：该应用程序将开发一种技术，即自适应光学扫描激光检眼镜，用于拍摄活体眼睛内部极其清晰的照片，清晰到可以看到单个细胞。该技术将用于研究年龄相关性黄斑变性和青光眼等疾病。它可以更早地检测视网膜疾病，更好地跟踪疾病进展以及视网膜疾病治疗的功效。