Abberior Infinity Line Upright 3D STED/Confocal Microscope

Abberior Infinity Line 正置 3D STED/共焦显微镜

• 批准号: 10632948
• 负责人: Anthony J Ricci
• 金额: $ 145.47万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-15 至 2024-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10632948
• 关键词: 3-Dimensional; Aging; Basic Science; Biological; Clinical; Collaborations; Core Facility; Data Analyses; Detection; Development; Devices; Diagnostic; Equipment; Experimental Designs; Fostering; Health; Image; Lasers; Light; Lighting; Maintenance; Malignant Neoplasms; Microscope; Mission; Noise; Optics; Otolaryngology; Pharmaceutical Preparations; Positioning Attribute; Postdoctoral Fellow; Research; Research Personnel; Resolution; Resources; Rotation; Running; Schedule; Scientist; Services; Signal Transduction; System; Technical Expertise; Technology; Training; United States National Institutes of Health; Work; adaptive optics; detector; experience; fluorophore; imaging system; improved; live cell imaging; nervous system disorder; next generation; sensory integration; student participation; superresolution imaging; technology development; ultra high resolution

Abstract: The Department of Otolaryngology has active research across all of its divisions. The department has run a core facility for over 12 years that is accessible to more than 100 researchers within our department. The core facility is a training facility that fosters collaboration across technical expertise and biological questions. We are requesting the purchase of a super- resolution imaging system that will be placed into this core facility where training, scheduling and maintenance can be managed by experienced staff. The Abberior STED system incorporates the most up-to-date technology to provide the most versatile super- resolution system available. The use of adaptive optics and adaptive illumination allows for high-resolution live cell imaging. The incorporation of avalanche diodes provides the most sensitive detectors available. Quad scanners maintain beam positioning and alignment while providing image rotation. Matrix array spectral detection offers the next generation of improved signal to noise by removing out focus light. STED FLIM allows for lower power laser to improve live-cell imaging. It further provides optical colocalization by using a single light path for multiple emissions. Multiple emissions are possible by separating fluorophores by lifetime rather than wavelength. There are no existing systems at Stanford so this new device will be critical for many scientists both within and outside of our department. Work within our department spans from basic science to translational to clinical. It ranges from cancer to aging, to neurological disorders, sensory integration and drug and diagnostic development. It is directly in line with the mission of NIH in terms of a primary focus on health-related research and training of the next-generation of scientists. Our core facility also serves as a training facility where we engage students, postdocs, residents, etc. and train them on the first principles of the technology development, experimental design and data analysis. Finally, the Department of Otolaryngology supports the core facility and will support this new addition by providing the space and the resources needed for staff as well as maintenance, service and oversight of the equipment.

抽象的： 耳鼻喉科的所有部门都在积极开展研究。这 该部门运营核心设施已超过 12 年，可供 100 多名人员使用 我们部门的研究人员。核心设施是培养人才的培训设施 跨技术专业知识和生物学问题的合作。我们要求 购买超分辨率成像系统并将其安装到该核心设施中 经验丰富的员工可以管理培训、日程安排和维护。这 Abberior STED 系统采用了最新的技术，提供最先进的 提供多功能超分辨率系统。使用自适应光学和自适应 照明可实现高分辨率活细胞成像。雪崩的合并 二极管提供最灵敏的探测器。四扫描仪保持光束 定位和对齐，同时提供图像旋转。矩阵阵列光谱检测 通过去除聚焦光，提供下一代改进的信噪比。斯泰德 FLIM 允许使用较低功率的激光来改善活细胞成像。它还提供光学 通过使用单个光路进行多次发射进行共定位。多重排放是 通过寿命而不是波长来分离荧光团是可能的。没有 斯坦福大学现有的系统因此这种新设备对于许多科学家来说都至关重要 我们部门内部和外部。我们部门的工作范围涵盖基础工作 科学到转化到临床。它的范围从癌症到衰老，再到神经系统疾病 疾病、感觉统合以及药物和诊断开发。是直接排队的 NIH 的使命是主要关注与健康相关的研究和培训 下一代科学家的。我们的核心设施还充当培训设施，其中 我们让学生、博士后、住院医师等参与进来，并对他们进行基本原则的培训 技术开发、实验设计和数据分析。最后，部门 耳鼻喉科支持核心设施，并将通过提供以下服务来支持这一新增设施： 工作人员所需的空间和资源以及维护、服务和 设备的监督。