Commercial translation of high-density carbon fiber electrode arrays for multi-modal analysis of neural microcircuits

用于神经微电路多模态分析的高密度碳纤维电极阵列的商业转化

基本信息

批准号：
10761217
负责人：
Rajmohan Bhandari
金额：
$ 148.76万
依托单位：
BLACKROCK MICROSYSTEMS
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-18 至 2025-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10761217
关键词：
3-Dimensional Acceleration Acute Address Adopted Aging Animal Experimentation Animal Model Animals BRAIN initiative Basic Science Biological Brain Businesses Carbon Catechols Characteristics Charge Chronic Cicatrix Collaborations Communities Development Devices Disease Documentation Electrodes Electron Microscopy Encapsulated Ethylenediamines Excision Fiber Functional disorder Future Geometry Goals Hazardous Substances Hemorrhage Human Implant In Vitro Industry Injections Laboratories Laboratory Research Legal patent Longevity Market Research Marketing Measurement Measures Mental disorders Methods Michigan Microelectrodes Microfabrication Modernization Motor Movement Neural Interconnection Neurons Neurosciences Neurotransmitters Noise Occupational Outcome Patients Penetration Performance Periodicity Phase Physiologic pulse Positioning Attribute Process Production Protocols documentation Quality Control Rattus Reproducibility Resolution Safety Sales Scanning Scheme Sensory Services Signal Transduction Silicon Site Small Business Innovation Research Grant Spectrum Analysis Surface System Techniques Technology Testing Time Tissues Translating Translations Traumatic injury Universities Utah Validation Visual biomaterial compatibility carbon fiber carcinogenicity clinical translation data acquisition density design electric impedance experience fabrication flexibility improved in vivo laboratory development loss of function manufacturability manufacture microsystems miniaturized device multi-electrode arrays multimodality nervous system disorder neural neural circuit neural implant neural prosthesis neurotechnology next generation novel operation pre-clinical research research and development statistics success systems research tool wireless

项目摘要

ABSTRACT The ability to measure and manipulate local brain circuit activity in living, behaving animals is essential to understanding the complexities of brain function and dysfunction. A novel, penetrating high-density carbon fiber electrode array composed of flexible, ultrathin conductive carbon microfibers was recently developed under the BRAIN Initiative to study neural microcircuit dynamics. In contrast to conventional microelectrode arrays, carbon fiber arrays are exceptionally biocompatible and produce minimal glial scarring resulting in exceptional proximity of the recording electrodes to neurons for unprecedented single-unit recording yield with improved electrode stability, high signal-to-noise recording, and high charge injection capacity (CIC) for superior stimulation. In addition, these arrays can be used to measure neurotransmitter or other biological compounds using fast-scan cyclic voltammetry (FSCV). Led by Blackrock Neurotech, a pioneer and industry leader in microelectrode array fabrication, the goal of this Direct-to-Phase II SBIR is to translate the novel laboratory fabricated high-density carbon fiber array (C-CFA) by the Chestek lab at the University of Michigan to a state-of-the-art commercial product (BRN-CFA) to enable broad dissemination of this powerful and versatile neurotechnology across the neuroscience community. Aim 1: Microfabrication and assembly of the BRN-CFA will dramatically improve the microfabrication process for the silicon support shuttles of the carbon fibers in the BRN-CFA by removing occupationally hazardous materials from the fabrication scheme and adopting a modern silicon-on-insulator wafer fabrication process to improve manufacturing safety, efficiency, and reliability. Array assembly and fabrication will occur under Blackrock’s Quality Management System. Aim 2: Performance validation and process transfer to Blackrock Neurotech will assess the optimized BRN-CFA electrical characteristics, robustness, stability, and longevity through in-vitro electrical, electrochemical testing, accelerated aging, and visual inspection. Analyses will be cross-validated against the C-CFA. The validated geometry, fabrication, and assembly processes will be transferred to Blackrock manufacturing to enable robust and reproducible production. Aim 3. In-vivo chronic performance validation of the BRN-CFA will cross-validate the BRN-CFA against the C- CFA in a 6-month in-vivo study in rat cortex to assess insertability and chronic performance of the devices. The successful outcome of this project will be the first commercially available BRN-CFA with exceptional performance for the study of local neural circuit dynamics. By addressing this critical, unmet need, the BRN-CFA product promises to accelerate basic scientific discovery of brain dynamics and the development of next-generation therapies.

抽象的测量和操纵活体、行为动物的局部脑回路活动的能力对于了解大脑功能和功能障碍的复杂性。由柔性超薄导电碳微纤维组成的电极阵列是最近在大脑计划研究神经微电路动力学与传统的微电极阵列相比，碳。纤维阵列具有出色的生物相容性，并产生最小的神经胶质疤痕，从而实现卓越的接近度将记录电极连接到神经元，通过改进的电极实现前所未有的单单元记录产量稳定性、高信噪比记录和高电荷注入能力 (CIC)，可实现卓越的刺激。此外，这些阵列可用于使用快速扫描来测量神经递质或其他生物化合物循环伏安法 (FSCV) 由微电极阵列领域的先驱和行业领导者 Blackrock Neurotech 领导。制造，这个直接进入第二阶段 SBIR 的目标是将新型实验室制造的高密度密歇根大学 Chestek 实验室将碳纤维阵列 (C-CFA) 应用于最先进的商业化产品（BRN-CFA），使这种强大且多功能的神经技术能够在整个世界范围内广泛传播目标 1：BRN-CFA 的微加工和组装将极大地改善神经科学界的神经科学界。 BRN-CFA 中碳纤维硅支撑梭的微加工工艺，通过去除制造方案中不含职业危险材料，并采用现代绝缘体上硅晶圆制造工艺，以提高制造安全性、效率和阵列组装和可靠性。制造将在贝莱德的质量管理体系下进行，目标 2：性能验证和流程。转移到 Blackrock Neurotech 将评估优化的 BRN-CFA 电气特性、稳健性、通过体外电气、电化学测试、加速老化和视觉测试来确定稳定性和寿命检查将根据 C-CFA 验证的几何形状、制造和交叉验证。装配工艺将转移至 Blackrock 制造，以实现稳健且可重复的生产。目标 3. BRN-CFA 的体内长期性能验证将交叉验证 BRN-CFA 与 C- CFA 在一项为期 6 个月的大鼠皮层体内研究中进行，以评估设备的可插入性和长期性能。该项目的成功成果将是第一个具有卓越性能的商用 BRN-CFA 为了研究局部神经回路动力学，BRN-CFA 产品解决了这一关键的、未满足的需求。承诺加速大脑动力学的基础科学发现和下一代的开发疗法。