Deciphering dopaminergic circuits required for food anticipatory activity in mice

破译小鼠食物预期活动所需的多巴胺能回路

基本信息

批准号：
10629786
负责人：
Andrew David Steele
金额：
$ 14.7万
依托单位：
CALIFORNIA STATE POLY U POMONA
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-05-01 至 2027-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10629786
关键词：
Activity Cycles Adaptive Behaviors Address Anatomy Animals Back Behavior Behavioral Biological Process Brain Caring Circadian Rhythms Corpus striatum structure Data Dependovirus Disease Dopamine Dorsal Eating Enzymes Exhibits Food Fright Genetic Genetic Identity Health Hunger Hyperphagia Hypothalamic structure Injections Knockout Mice Light Link LoxP-flanked allele Maps Measures Medial Mediating Medical Modernization Mus Nature Neurobiology Neurons Physiological Play Population Production Rattus Recording of previous events Regulation Research Role Signal Transduction Site Societies Stimulus Substantia nigra structure System Techniques Temperature Testing Time Translational Research Tyrosine 3-Monooxygenase United States Viral Vision behavior observation calbindin circadian conditional knockout cost designer receptors exclusively activated by designer drugs dopamine transporter dopaminergic neuron experimental study feeding feeding schedule genetic manipulation grasp light entrainment nerve supply neural circuit neurotransmission response restoration retrograde transport suprachiasmatic nucleus tool translational impact

项目摘要

Project Summary/Abstract Given the over-abundance of food in modern society, it is easy to lose sight of the importance of feeding to animals in nature. For most animals, food is extremely scarce and being wise to opportunities to eat is essential for survival. As such, the circadian system has evolved to receive a number of different stimuli— i.e., light, temperature, food, and even fear—to keep biological processes coordinated and allow for adaptation to ever changing conditions. The ability to tell time relative to feeding has a long history of research, going back more than one hundred years hundred years with behavioral observations of honeybees and rats. However, the neural circuitry behind adaptation of behavior to timed feeding has continually escaped our grasp. In this project, I will utilize conditional genetics, viral restorations, and chemogenetic tools to identify the dopamine population (both anatomically and genetically) that is required for the behavioral expression of food anticipatory activity in lab mice. From here, we will build out a more comprehensive characterization of how a select population of dopamine neurons projecting to the striatum can inform the time keeping systems of the brain that food is available.

项目概要/摘要现代社会食物过剩，人们很容易忽视喂养的重要性。对于自然界中的大多数动物来说，食物是极其稀缺的，明智地利用进食机会是很重要的。因此，昼夜节律系统已经进化到可以接受许多不同的刺激—— 即光、温度、食物，甚至恐惧——以保持生物过程和协调一致适应不断变化的条件的能力有着悠久的历史。研究，可以追溯到一百多年前，通过行为观察然而，蜜蜂和老鼠的行为适应定时喂食背后的神经回路已经发生了变化。在这个项目中，我将利用条件遗传学、病毒修复和化学遗传学工具来识别所需的多巴胺群体（解剖学和遗传学）从这里开始，我们将建立一个更多的实验小鼠食物预期活动的行为表达。全面表征选定的多巴胺神经元群如何投射到纹状体可以通知大脑的计时系统有食物可用。