H–2K-, H–2I- and H–2D- restricted hybridoma contact sensitivity effector cells

Mice primed with 4-hydroxy-3-nitrophenylacetyl-O-succini-mide (NP-O-Su) generate hapten-specific T cell-mediated contact or cutaneous hypersensitivity (CS) responses1. Analysis of the effector T cells mediating these in vivo responses suggested that multiple T-cell subpopulations were involved2. It has not been possible previously to separate these cell populations physically by conventional means. Therefore, to separate these cells and to provide a uniform source of CS effector cells for future analyses, we hybridized the CS effector cell population with AKR-derived BW5147 thymoma cells. We now describe five H–2-restricted hybridoma T-cell clones isolated from this fusion. Each hybridoma possesses haptenspecific CS activity and four also crossreact with 4-hydroxy-3-nitrophenylacetyl (NP) derivatives. Two of the hybridoma clones are specific for NP associated with H–2I gene products; two others for NP associated with H–2D products; and one for NP associated with H–2K gene products.

用4 - 羟基 - 3 - 硝基苯乙酰 - O - 琥珀酰亚胺（NP - O - Su）致敏的小鼠会产生半抗原特异性T细胞介导的接触性或皮肤超敏反应（CS）。对介导这些体内反应的效应T细胞的分析表明，涉及多个T细胞亚群。以前不可能通过常规方法对这些细胞群进行物理分离。因此，为了分离这些细胞，并为未来的分析提供均一的CS效应细胞来源，我们将CS效应细胞群与源自AKR的BW5147胸腺瘤细胞进行杂交。我们在此描述了从这次融合中分离出的5个H - 2限制性杂交瘤T细胞克隆。每个杂交瘤都具有半抗原特异性CS活性，其中4个还与4 - 羟基 - 3 - 硝基苯乙酰（NP）衍生物发生交叉反应。两个杂交瘤克隆对与H - 2I基因产物相关的NP具有特异性；另外两个对与H - 2D产物相关的NP具有特异性；还有一个对与H - 2K基因产物相关的NP具有特异性。