Ultra-performance liquid chromatography - triple quadrupole mass spectrometry system

超高效液相色谱-三重四极杆质谱系统

• 批准号: 10629514
• 负责人: H-H. Sherry CHOW
• 金额: $ 50.44万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-15 至 2024-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10629514
• 关键词: Aging; Analytical Chemistry; Arizona; Basic Science; Biological; Biological Assay; Biological Availability; Biological Markers; Biomedical Research; Cancer Center; Cancer Center Support Grant; Carcinogens; Clinical Research; Complex; Development; Disease; Drug or chemical Tissue Distribution; Environment; Equipment; Fostering; Funding; Grant Review; Institution; Liquid Chromatography; Manufacturer; Mass Chromatography; Mass Spectrum Analysis; Measurement; Metabolic; National Cancer Institute; Nutraceutical; Peptides; Performance; Pharmaceutical Preparations; Research Personnel; Resource Sharing; Risk; Running; Sampling; Scanning; Specimen; Speed; System; Time; Tissues; Toxicant exposure; Translational Research; United States National Institutes of Health; Universities; Validation; bioactive food component; cost effectiveness; improved; instrument; interest; lipidomics; liquid chromatography mass spectrometry; metabolomics; multidisciplinary; multiple reaction monitoring; novel therapeutic intervention; novel therapeutics; preventive intervention; quantum; small molecule; success; toxicant

PROJECT SUMMARY The University of Arizona is requesting funds for the acquisition of an ultra-performance liquid chromatography – triple quadrupole mass spectrometry system (triple quadrupole UPLC/MS). The instrument will be operated within the framework of the University of Arizona Cancer Center, Analytical Chemistry Shared Resource (UACC, ACSR) to support 12 NIH-funded researchers, including 10 UACC investigators, and new users. ACSR is a shared resource funded by the UACC Cancer Center Support Grant (CCSG) since 2001 to provide centralized support in performing chromatography-mass spectrometry based assays for quantitative measurements of drugs, novel therapeutics, nutraceuticals, bioactive food components, carcinogens, toxicants, and endogenous metabolites in various biological specimens, as well as qualitative studies in untargeted metabolomics and lipidomics. It has a proven track record of providing support to UACC investigators and campus-wide users and has received an “Outstanding” merit in the National Cancer Institute’s CCSG review. Users listed in this application have past and/or ongoing projects supported by the ACSR. The triple quadrupole LC/MS is considered the gold standard for rapid development, validation, and implementation of analytical chemistry assays to enable highly sensitive and specific quantitative analysis of small molecule analytes in complex biological samples. The primary need for the instrument is to replace an aging instrument (Quantum Ultra triple quad acquired in 2005) no longer supported by the manufacturer. ACSR supports projects from an average of 30 investigators annually and is still using this aging equipment today to support roughly 40% of projects. In addition to replacing the aging instrument, the requested instrument also offers an improved assay sensitivity into the ppt range and beyond and has a wider mass range expanding the analysis of peptides. With the ultrahigh scan speeds combined with dynamic multiple reaction monitoring, the requested instrument can also support comprehensive, high-throughput, targeted lipidomics analysis. Finally, the upgraded UPLC will enhance both capability and capacity, via shorten run time and superior separation compared to our current system. The instrument will support the assessment of the systemic bioavailability and tissue distribution of novel therapeutics and preventive interventions, the measurements of the extent of systemic and target tissue carcinogen/toxicant exposure, the measurement of endogenous metabolites in biofluids and tissues as surrogate risk and/or end- point biomarkers and for assessment of metabolic changes to define the mechanisms underlying the disease of interest and to develop new strategies for treatment. Incorporation of the requested instrument into a well- established shared resource will promote cost effectiveness, encourage optimal sharing among users, and foster a collaborative multidisciplinary environment. We strongly believe that the requested instrument will have a significant impact on NIH-funded basic, translational, and clinical research at the University of Arizona and contribute to the institution’s continued success in biomedical research.

项目概要 亚利桑那大学正在申请资金购买超高效液相色谱仪 – 三重四极杆质谱系统（三重四极杆 UPLC/MS）。 在亚利桑那大学癌症中心的框架内，分析化学共享资源（UACC， ACSR）支持 12 名 NIH 资助的研究人员，其中包括 10 名 UACC 研究人员，ACSR 是一个新用户。 自 2001 年起由 UACC 癌症中心支持补助金 (CCSG) 资助的共享资源，以提供集中 支持进行基于色谱-质谱分析的定量测量 治疗剂、新药、营养保健品、生物活性食品成分、致癌物、毒物和内源性物质 各种生物样本中的代谢物，以及非靶向代谢组学的定性研究和 它在为 UACC 研究人员和全校用户提供支持方面有着良好的记录。 已在美国国家癌症研究所的 CCSG 用户评审中获得“杰出”奖。 应用程序有由 ACSR 支持的过去和/或正在进行的项目。 被认为是分析化学快速开发、验证和实施的黄金标准 能够对复杂的小分子分析物进行高度灵敏和特异性的定量分析 该仪器的主要需求是更换老化仪器（Quantum Ultra Triple）。 2005 年收购的 Quad）不再受到制造商的支持，平均支持的项目为 每年有 30 名研究人员参与，至今仍在使用这种老化的设备来支持大约 40% 的项目。 除了更换老化仪器外，所需的仪器还提供了改进的分析灵敏度 进入 ppt 范围及以上，并具有更宽的质量范围，扩大了肽的分析范围。 扫描速度与动态多反应监测相结合，所要求的仪器还可以支持 最后，升级后的 UPLC 将增强这两者。 与我们当前的系统相比，通过缩短运行时间和出色的分离来实现能力和容量。 仪器将支持评估治疗性小说的全身生物利用度和组织分布 预防和干预，测量全身和靶组织致癌物/毒物的程度 暴露，测量生物体液和组织中的内源代谢物作为替代风险和/或最终风险 点生物标志物和代谢变化评估，以确定疾病的潜在机制 兴趣并制定新的治疗策略。 建立共享资源将提高成本效益，鼓励用户之间的最佳共享，并促进 我们坚信所需的工具将具有一个协作的多学科环境。 对 NIH 资助的亚利桑那大学基础、转化和临床研究产生重大影响 为该机构在生物医学研究方面的持续成功做出贡献。